Cell structure 2.1.1 Flashcards
What does magnification mean?
How many times larger an image appears relative to the size of the objects actual size
What does resolution mean?
The ability of an optical system to distinguish between two adjacent points
What is the formula for magnification?
What is the maximum effective magnification of a light microscope?
x1,500
What is the maximum effective magnification of a transmission electron microscope?
x500,000
What is the maximum resolution of a light microscope?
200nm
What is the maximum resolution of a transmission electron microscope?
0.1nm
What is the decreasing order of units used to measure cells?
What is the maximum effective magnification of a scanning electron microscope?
x200,000
What is the maximum resolution of a scanning electron microscope?
between 3 and 10nm
What are the advantages of a light microscope?
- relatively cheap
- easy to use
- portable
- ability to use on whole living specimens as well as sectioned
What are the advantages of a transmission scanning microscope?
- High magnification and resolution which produces detailed images of cells/organelles
- Allows internal structures of cells/organelles to be seen
What are the advantages of a scanning electron microscope?
- Produces a 3D image
- Reveals details of contours and cell/tissue arrangements
What are the limitations of a light microscope?
- Low magnification and resolution which means that many organelles cannot be seen
- Samples often need staining to help distinguish different features
What are the limitations of a transmission scanning electron?
- The specimen has to be chemically fixed by dehydration and staining which is why it cannot be used on living samples
- need a great deal of skill and training to use
- are large and very expensive
- Image cant be viewed directly