Cell structure 2.1.1 Flashcards

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1
Q

What does magnification mean?

A

How many times larger an image appears relative to the size of the objects actual size

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2
Q

What does resolution mean?

A

The ability of an optical system to distinguish between two adjacent points

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3
Q

What is the formula for magnification?

A
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4
Q

What is the maximum effective magnification of a light microscope?

A

x1,500

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5
Q

What is the maximum effective magnification of a transmission electron microscope?

A

x500,000

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6
Q

What is the maximum resolution of a light microscope?

A

200nm

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7
Q

What is the maximum resolution of a transmission electron microscope?

A

0.1nm

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8
Q

What is the decreasing order of units used to measure cells?

A
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9
Q

What is the maximum effective magnification of a scanning electron microscope?

A

x200,000

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10
Q

What is the maximum resolution of a scanning electron microscope?

A

between 3 and 10nm

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11
Q

What are the advantages of a light microscope?

A
  • relatively cheap
  • easy to use
  • portable
  • ability to use on whole living specimens as well as sectioned
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12
Q

What are the advantages of a transmission scanning microscope?

A
  • High magnification and resolution which produces detailed images of cells/organelles
  • Allows internal structures of cells/organelles to be seen
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13
Q

What are the advantages of a scanning electron microscope?

A
  • Produces a 3D image
  • Reveals details of contours and cell/tissue arrangements
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14
Q

What are the limitations of a light microscope?

A
  • Low magnification and resolution which means that many organelles cannot be seen
  • Samples often need staining to help distinguish different features
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15
Q

What are the limitations of a transmission scanning electron?

A
  • The specimen has to be chemically fixed by dehydration and staining which is why it cannot be used on living samples
  • need a great deal of skill and training to use
  • are large and very expensive
  • Image cant be viewed directly
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16
Q

What are the limitations of a scanning electron microscope?

A
  • Cannot be used on living samples
  • are large and very expensive
  • need a great deal of skill and training to use
  • only reveal the surface detail or section of a specimen
  • Images cant be viewed directly
17
Q

Label the microscope.

A

Labels:

  1. Eyepiece
  2. Body tube
  3. High power objective lens
  4. Low power objective lens
  5. Stage
  6. Condenser
  7. Mirror
  8. Base
  9. Coarse adjusment screw
  10. Fine adjustment screw
  11. Arm
18
Q

Label the light diagram of a light microscope.

A

Labels:

  1. Eye
  2. Objective lens
  3. Specimen
  4. Condenser lens
  5. Eyepiece
19
Q
A