Cell structure Flashcards
define the term magnification
magnification describes how much bigger an image appears compared with the original object.
define the term resolution
resolution is the ability of an optical instrument to see or produce an image that shows fine detail clearly .
what are the advantages of using an optical microscope
-cheap
-easy to use
-portable and to be used in fields as well as labs
Able to study whole large specimens
what is the maximum magnification of an optical microscope
x1500, or in some cases up to x2000
what is the calculation of magnification
total magnification= magnifying power of the objective lens x magnifying power of the eyepiece lens
what is the wavelength of visible visible light and what does it means in terms of the resolution maximum
the wavelength of visible light ranges from 400 to 700nm so structures closer than 200nm will appear as one object (resolution is above 200nm)
what do you call a photograph of an optical microscope view
photomicrograph
how does the laser scanning microscope work
laser scanning microscopes are also called confocal microscopes
- they use laser light to scan an object point by point and assemble, by computer, the pixel information into one image displayed on a computer screen.
-the images are high resolution and show high contrast.
-these microscopes have depth selectivity and can focus on structures at different depths within a specimen. such microscopy can therefore be used to clearly observe whole living specimens, as well as cells.
-they are used in the medical profession, for example, to observe fungal filaments within the cornea of the eye of a patient with a fungal corneal infection, in order to give a swift diagnosis.
how do electron microscopes work
electron microscopes use a beam of fast-traveling electrons with a wavelength of about 0.004nm. this means they have much greater magnification and resolution.
the electrons are fired from a cathode and focused by magnets rather than glass magnets, onto a screen or photographic plate. fast traveling electrons have a wavelength of around 125 000 times smaller than that of the central part of the visible light spectrum. this accounts for the better resolution and magnification.
describe the differences between the two types of electron microscopes
transmission electron microscopes
- the specimen has to be chemically fixed, dehydrated, and stained. the beam of electrons passes through the specimen, which is stained with metal salts. some electrons pass through and are focused onto the screen or photographic plate. the electrons form a 2D black-and-white image. when photographed this is called an electron micrograph. TEM microscopes can produce a magnification of up to 2 million x, and a new generation is being developed that can magnify up to 50 million times.
scanning electron microscopes
these were developed during the 1960s. electrons do not pass through the specimen, which is whole, but cause secondary electrons to bounce off the specimen surface and be focused onto a screen. this gives this gives a 3D image with a magnification from up to 15x up to 200,000x. the image is black and white, but computer software programs can add false colour, however, the specimen still has to be placed in a vacuum and is often coated with a fine film of metal.
how do you observe unstained specimens
Many biological structures, including single celled organisms such as paramecium, are colorless and transparent. Some microscopes use light inference, rather than light absorption, in order to produce a clear image without staining. Some use a dark background against which the illuminated specimen shows up. These microscopes are particularly useful for studying living specimens.
give 3 examples of stain types used for microscopy and what they do
Stains are colored chemicals that bind to molecules in or on the specimen, making the specimen easy to se. Methylene blue is an all purpose stain. Some stains bind to specific cell structures, staining each structure differently so the structure can be easily identified within a single preperation. This is called differential staining. 4 examples of this are
-acetic orcein binds to DNA and stains chromosomes a dark red
-eosin stains the cytoplasm
-Sudan red stains lipids
-iodine in potassium iodide solution stains the cellulose in plant cell walls yellow, and starch granules blue/black (these will look violet under a microscope)
How do experts prepare permanently fixed slide
-dehydration of the specimen
-embedding them in wax to prevent distortion when slicing
-using a special instrument to make very thin slices called sections- these are stained and mounted in a special chemical to preserve them
What is the magnification equation
Magnification = image size/ actual size
Outline the properties (organelles) of eukaryotic cells
All animal, protoctist, plant and fungal cells are eukaryotic. This means they all have:
-a nucleus surrounded by a nuclear envelope and containing DNA organized and wound into linear chromosomes
-an area inside the nucleus called the nucleolus, containing RNA, where chromosomes unwind; the nucleolus is involved in production of ribosomes.
-jelly like cytoplasm in which organelles are suspended
-a cytoskeleton- a network of protein filaments (actin or microtubules) within the cytoplasm that move organelles from place to place inside cells.
-a plasma membrane (cell surface membrane)
-membrane bound organelles, other than the nucleus, such as mitochondria, the Golgi apparatus and endoplasmic reticulum
-small vesicles
-ribosomes, which are organelles without membranes, where proteins are assembled.