Cell Staining terminology Flashcards
what does H and E stand for?
hematoxylin and eosin
describe H and E stain
most commonly used dye combination; hametoxylin is a nuclear dye and eosin is a cytoplasmic dye
describe trichrome stain
a 3 color stain, the red and blue components are acidic dyes while the nuclear dye is hematoxylin, a basic dye
what is meant by acidophilic cell staining?
loves acid; is a substance that is usually basic in nature that is easily stainable with acidic (anionic) dyes; an example is cell cytoplasm, which is readily stainable with the acidic (anionic) dye eosin
what is an anion?
a negatively charged dye or tissue component; referred to as acidic and attract basic substances
what is meant by basophilic cell staining?
a substance that is acidic in nature that is easily stainable with basic (cationic) dyes; example is the cell nucleus, which is easily stainable with the basic (cationic) dye hematoxylin
what is a cation?
a positively charged dye or tissue component; referred to as basic because they attract acidic substances and dyes
what is meant by euchromatic/euchromatin?
the parts of the chromosomes that, during interphase, as uncoiled dispersed threads and therefore not stained by ordinary dyes (can’t really see the chromatin, can see nucleolus)
what is meant by heterochromatic/heterochromatin?
the part of the chromosome that remains tightly coiled and condensed during interphase and stains readily; can see lots of little clumps
describe nuclear pyknosis
pyknosis is condensation and reduction in the size of the cell or its nucleus; nuclear pyknosis is a pathologic stages of necrosis
