cell organization? forget what lecture is called Flashcards

1
Q

protein in membrane

A

N side extracellular, C side intracellular, alpha helix inside membrane

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2
Q

functions of membrane proteins (6)

A

transport, enzymatic activity, signal transduction, cell-cell recognition, intercellular joining/adhesing, attachment to cytoskeleton and extracellular matrix

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3
Q

peripheral proteins

A

, sit on other proteins attached to plasma membrane

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4
Q

solution

A

solvent the liquid, in which the solute is dissolved

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5
Q

solute

A

could be molecule

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6
Q

isotonic solutions

A

when comparing two solutions, same concentrations

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7
Q

hypotonic solution

A

when comparing two solutions, one with lower concentration

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8
Q

hypertonic solution

A

when comparing two solutions, one with higher concentration

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9
Q

H-bonds in water

A

electronegativity gives attraction for H-bonds, dissolve things (ex. NaCl); hydration shells

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10
Q

passive transport

A

diffusion, osmosis

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11
Q

diffusion

A

the movement of solutes down a concentration gradient, a permeable membrane does not impede diffusion, diffuse until equilibrium

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12
Q

osmosis

A

the movement of WATER along a concentration gradient through a semi or selectively permeable membrane to one or more solutes

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13
Q

osmosis in red blood cells

A

cell will burst/lyse in hypotonic solution, cell will shrivel in hypertonic solution, *normal in isotonic

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14
Q

osmosis in plant cells

A

cell turgid *(normal) in hypotonic solution because pressure in cell wall gives turgidy, cell flaccid in isotonic solution, cell plasmolyzed in hypertonic solution (shrinks)

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15
Q

unicellular organisms and osmoregulation

A

hypotonic outside, hypertonic inside, collects water in contractile vacuole (when full, will expel water)

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16
Q

simple/passive diffusion

A

passes through membrane (semi, selectively, or permeable)

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17
Q

passive facilitated diffusion

A

through channel/transport protein in membrane

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18
Q

active transport

A

require ATP and energy to bring in solute against concentration gradient

19
Q

polar molecules permeability

A

amino acids, sugars, and charges substances such as ions, cannot easily diffuse across lipid bilayers even if concentration gradient exists

20
Q

high permeable molecules

A

small, non-polar molecules: ex. O2, CO2, N2

21
Q

permeable molecules

A

small uncharged polar molecules: ex. H2O, glycerol

22
Q

semi-permeable molecules

A

large uncharged polar molecules: ex. glucose, sucrose

23
Q

low permeable molecules

A

ions, Na+, Cl-, K+. Ca2+

24
Q

how polar molecules enter cells

A

facilitated diffusion, involving channel proteins and carrier/transport proteins (in plasma membrane)

25
Q

channel protein structure

A

membrane proteins that form channels through the plasma membrane, lined with polar (hydrophilic) amino acids, non-polar (hydrophobic) amino acids face the outside of the channel, towards fatty acid tails of lipid molecules

26
Q

Aquaporins

A

water channel proteins, main ways water moves through membrane

27
Q

ion channels

A

ion channels are GATED, gates open or close to facilitate the movement of ions, when opened, ions flow down concentration gradient

28
Q

potassium channel

A

ion channel that allows passage of potassium,

29
Q

transport/carrier proteins

A

form channels but they are also specifically bind the transported substance, have binding sites, allows transport to be selective

30
Q

transport/channel proteins and selectivity

A

transport of sugars and amino acids, allow diffusion in both directions, concentration gradient can be maintained by metabolizing the transported substance once it enters the cell ex. glucose metabolized when enters the cell so glucose levels remain low and movement continues

31
Q

active transport

A

requires the expenditure of energy, moves AGAINST concentrations (from low to high), energy direct or indirectly from ATP hydrolysis

32
Q

direction of active transport

A

uniport, symport, antiport

33
Q

uniport transporters

A

move a single type of solute (ex. calcium ions) in one direction against its concentration gradient

34
Q

symport transporters

A

move two solutes in the same direction, only ONE of which is against its concentration gradient (ex. amino cid transport coupled with sodium ion transport, sodium moves down, amino acids move against)

35
Q

antiport transporters

A

move two solutes in opposite directions, one into the cell, one out of the cell, both of which are AGAINST their concentration gradients (ex. sodium potassium pump, sodium out, potassium in, each 1 ATP 3 Na+ out and 2 K+ in)

36
Q

sodium transport across membrane

A

cells use loss of energy from sodium movement through protein to push in K+; if sodium channel is open, sodium goes into cell until equilibrium is reached; inside of cell reaches 15mM then pushes extra sodium out with ATP, while K+ is pushed into cell (opposite direction, against concentration gradient)

37
Q

sodium-potassium pump process

A

3 sodium ions bind to pump, Na+ binding stimulates phosphorylation (phosphate and ADP) by ATP, this causes protein to change shape and Na+ is expelled, then + binds on extracellular side and triggers release of phosphate group, protein goes back to original shape and 2 K+ are pumped in (cycle repeats!)

38
Q

electrogenic pump (proton pump)

A

moves ions against an ionic gradient aand generate a voltage difference across the membrane; proton pump causes + or - difference; ACTIVE

39
Q

cotransport

A

transport mechanism for one ion or molecule can help out another transporter to move and different ion or molecule

40
Q

coupled transport processes

A

cooperate to facilitate the movement of ions or molecules across membranes, requires energy (ACTIVE), cotransport

41
Q

active transport by concentration gradient

A

electrogenic pumps can be used to help drive symporters such as the sucrose transporter by maintaining an ionic gradient

42
Q

active transport by concentration gradient examples

A

sucrose-proton cotransporter; glucose-sodium/potassium pump cotransporter

43
Q

bulk transport across membranes

A

food or other particle is engulfed by membrane and is put in food vacuole, food particle + extracellular fluid/solutes enter via endocytosis

44
Q

receptor endocytosis

A

a coated pit and a coated vesicle formed during receptor-mediated endocytosis