Cell Organelles Lab slides

Question 1

Answer 1

EM of Cilla

You can see the basal body at 2, which are microtubules arranged as triplets

basal body NUCLEATES the cillia

can tell cillia because it has 9+2 arrangement

Question 2

Answer 2

These arrows are showing intercellular boarders

Question 3

Answer 3

Arrow pointing to the nuclus but look athedots you can see the NUCLEOULUS THE DOTS

Question 4

Answer 4

Lipofucsion

tertiary lyzosomes

its the like pink outside of the nuclus

Question 5

What is this pointing to and what is the stain used?

Answer 5

This is a muscle cell but pointing to the nucleus

HE- purple blue color

Question 6

Answer 6

cannot see the nucleous, but you can see HCin center or peripheral clumped and EC unwound

Question 7

Answer 7

IMPORTANT look at the PA= pars amorpha aka amorphos area which is the DNA which gives rise to the RNA

Question 8

Identify

Answer 8

Mitochndria

Nucleus

Question 9

Identify

Answer 9

Mitochondria

nuceus circles inside

Question 10

Identify

what microscope is used ?

Answer 10

Golgi

know this because with silver always stains BLACK

Light microscope

Question 11

Identify

what microscope is used ?

Answer 11

Golgi

Electron Microscope

You can tell because look at the vesicles numbered 123 they are from the golgi which gives rise to them from its trans face

Question 12

Identify

what microscope is used ?

Answer 12

RER to the right and smooth er to the lefts

EM

Question 13

WHAT STAIN WHAT IS THIS , IS THERE A NUCLEUS ? WHERE IS THIS

Answer 13

ROUNDED GLYCOGEN GRANULES LOCATED IN THE LIVER, HAVE NO MEMBRANE !Non membrane bound inclusion

NUCLEUS VISABLE IN THE CENTER SURROUNDED BY GLYCOGEN GRANULES stained red with Best’s CAMINE STAIN

Question 14

WHAT IS THIS

WHERE IS IT

WHAT TYPE OF STAIN

Answer 14

GLYCOGEN GRANULES SURROUNDED BY NUCLEUS IN THE LIVER

PAS STAIN -STAINS GYCOGEN BRIGHT PURPLE/PINK

Question 15

IDENTIFY

Answer 15

LIPID

POINTING TO NUCLEUS

ADIPOCYTES

NOT MEMBRANE BOUND

Question 16

IDENTIFY A AND B

Answer 16

ACTIN FILAMENTS

BOTH Imunnoflurorescence Microgrph of Cultured FIBROBLAST

A. Stationary fibroblast

B- Migratory Fibroblast - cell is moving so stains darker brighter as it is moving Le, by cell wall condensed

stained with anti-actin antibodies

Question 17

what phase

Answer 17

METAPHASE

Question 18

Answer 18

Nuclear membrane has disappeard

prohase

Question 19

Identify

what type of Microscope?

Answer 19

Light Microscope

Methaphase

Question 20

Identify

what type of Microscope?

Answer 20

Anaphase

LM

Question 21

Identify

what type of Microscope?

Answer 21

TeloPhase

Lm

Question 22

Identify

what type of Microscope?

what is that streak in the center

Answer 22

Anaphase

EM

Microtubules!!!

Question 23

Identify

what type of Microscope?

Answer 23

Lipids - you can see the cirlces witht he dark perimeter in NOT A MEMBRANE , lipids have no membrade

see RER