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HIST BIOM > Cell Organelles Lab slides > Flashcards

Cell Organelles Lab slides Flashcards

1
Q
A

EM of Cilla

You can see the basal body at 2, which are microtubules arranged as triplets

basal body NUCLEATES the cillia

can tell cillia because it has 9+2 arrangement

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2
Q
A

These arrows are showing intercellular boarders

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3
Q
A

Arrow pointing to the nuclus but look athedots you can see the NUCLEOULUS THE DOTS

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4
Q
A

Lipofucsion

tertiary lyzosomes

its the like pink outside of the nuclus

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5
Q

What is this pointing to and what is the stain used?

A

This is a muscle cell but pointing to the nucleus

HE- purple blue color

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6
Q
A

cannot see the nucleous, but you can see HCin center or peripheral clumped and EC unwound

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7
Q
A

IMPORTANT look at the PA= pars amorpha aka amorphos area which is the DNA which gives rise to the RNA

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8
Q

Identify

A

Mitochndria

Nucleus

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9
Q

Identify

A

Mitochondria

nuceus circles inside

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10
Q

Identify

what microscope is used ?

A

Golgi

know this because with silver always stains BLACK

Light microscope

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11
Q

Identify

what microscope is used ?

A

Golgi

Electron Microscope

You can tell because look at the vesicles numbered 123 they are from the golgi which gives rise to them from its trans face

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12
Q

Identify

what microscope is used ?

A

RER to the right and smooth er to the lefts

EM

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13
Q

WHAT STAIN WHAT IS THIS , IS THERE A NUCLEUS ? WHERE IS THIS

A

ROUNDED GLYCOGEN GRANULES LOCATED IN THE LIVER, HAVE NO MEMBRANE !Non membrane bound inclusion

NUCLEUS VISABLE IN THE CENTER SURROUNDED BY GLYCOGEN GRANULES stained red with Best’s CAMINE STAIN

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14
Q

WHAT IS THIS

WHERE IS IT

WHAT TYPE OF STAIN

A

GLYCOGEN GRANULES SURROUNDED BY NUCLEUS IN THE LIVER

PAS STAIN -STAINS GYCOGEN BRIGHT PURPLE/PINK

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15
Q

IDENTIFY

A

LIPID

POINTING TO NUCLEUS

ADIPOCYTES

NOT MEMBRANE BOUND

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16
Q

IDENTIFY A AND B

A

ACTIN FILAMENTS

BOTH Imunnoflurorescence Microgrph of Cultured FIBROBLAST

A. Stationary fibroblast

B- Migratory Fibroblast - cell is moving so stains darker brighter as it is moving Le, by cell wall condensed

stained with anti-actin antibodies

17
Q

what phase

A

METAPHASE

18
Q
A

Nuclear membrane has disappeard

prohase

19
Q

Identify

what type of Microscope?

A

Light Microscope

Methaphase

20
Q

Identify

what type of Microscope?

A

Anaphase

LM

21
Q

Identify

what type of Microscope?

A

TeloPhase

Lm

22
Q

Identify

what type of Microscope?

what is that streak in the center

A

Anaphase

EM

Microtubules!!!

23
Q

Identify

what type of Microscope?

A

Lipids - you can see the cirlces witht he dark perimeter in NOT A MEMBRANE , lipids have no membrade

see RER

24
Q
A

HIST BIOM (2 decks)

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