Cell Morphology

Question 1

Flocculent

Answer 1

Floating chunks or pieces

Question 2

Friable

Answer 2

Crusty

Question 3

Spreading edge

Answer 3

Effuse: motile organisms seeming to spread out from the center

Question 4

Pigmented/Translucent/Opaque

Answer 4

Color and light observations of CFU

Question 5

Pellicle

Answer 5

Growth floats on top

Question 6

Sediment

Answer 6

growth sinks to bottom

Question 7

Turbid: uniform fine turbidity

Answer 7

cloudiness throughout broth

Question 8

CFU stands for…

Answer 8

Colony Forming Units (Colony = visible group of cells)

Question 9

Ring

Answer 9

Growth on top around the sides of the test tube

Question 10

Filiform

Answer 10

Dense and Opaque with smooth edge

Question 11

Agar Slants

Answer 11

Used for cultivation, and storage of stock cultures for a long period of time

-anaerobic bacteria can be grown by stabbing culture
-aerobic bacteria grows on top

Question 12

Shape: (overarching shape)

Answer 12

Round, Irregular, Punctiform (pinpoint dots)

Question 13

Margin

Answer 13

Edge of colony

Question 14

smooth edge, no irregularities

Answer 14

Entire

Question 15

wavy edge

Answer 15

Undulate

Question 16

Lobed edge

Answer 16

Lobate

Question 17

unbranched strands

Answer 17

Filamentous

Question 18

branched like roots CFU formation

Answer 18

Rhizoid

Question 19

Elevation

Answer 19

flat, raised, convex, pulvinate, umbonate

Question 20

pulvinate

Answer 20

convex

Question 21

umbonate

Answer 21

raised just in the center of colony

Question 22

Texture

Answer 22

moist, mucoid, butyrous, dry, shiny, dull

Question 23

butyrous

Answer 23

butter-like

Question 24

Spread Plate Method

Answer 24

Serial dilutions used to dilute broth culture into growing individual colonies - can create a pure culture

Dense culture inoculated plate to form bacteria lawn (Confluent). Then used to test antibacterial stuff and meds.

Question 25

Bacteria Lawn

Answer 25

Confluent

Question 26

Serial Dilution

Answer 26

CFU/mL = #colonies counted / (Volume of dilution plated x dilution factor)

Question 27

How many colonies on plate?

Answer 27

TMTC - too many to count > 300
TFTC - too few to count < 30
30 - 300 - ideal number

Question 28

Liquid or broth culture

Answer 28

• Used to increase number of cells. Cells grow exponentially.
  X * 2^N
  x = initial cells
  N = generation
• transformation (addition of DNA to organism)

Question 29

Agar Plate

Answer 29

-Streaking a plate: growing isolated colonies for pure culture creation
- characterization of colony

Question 30

Aseptic Technique 3 levels

Answer 30

without contamination
1- Decontamination -(minimal PPE, wash with soap and water, handle without gloves)
2 - Disinfection - low, med, high levels within. Typically liquid chemical but can be solid, gas, heat & UV.
3 - Sterilization - complete elimination of viable organisms + spores (hard to destroy)

Question 31

Autoclave

Answer 31

Steam-sterilization

Question 32

Pure Culture

Answer 32

One organism - can be obtained by streaking

Question 33

Mixed Culture

Answer 33

Culture with 2 or more different organisms.
- always assumed before isolation