Cell integrity Flashcards
What is the redox reaction for NADH and how much energy is released?
NADH + H+ + 0.5O2 > NAD+ + H2O
-223KJ/MOL
What is the redox reaction for FADH2 and how much energy is released?
FADH2 + H+ + 0.5O2 > FAD+ + H2O
-170 KJ/MOL
What are the four membrane proteins in the ETC?
> complex I - NADH-Q-Reductase complex
complex II - Succinate dehydrogenase
(mobile carrier) ubiquinone - coenzyme Q
Complex III - Cytochrome c reductase complex
(mobile carrier) cytochrome C
Complex IV - cytochrome C oxidase complex
What does cyanide do?
bind with high affinity to the ferric Fe3+ form of the haem group in the cytochrome oxidase complex blocking the final step of the ETC
What does azide do (N3-)?
bind with high affinity to the ferric Fe3+ form of the haem group in the cytochrome oxidase complex blocking the final step of the ETC
What does malonate do?
Resembles succinate
competitive inhibitor for succinate dehydrogenase
- stops flow of electrons to ubiquinone by inhibiting oxidation to fumarate
What does rotenone do?
Inhibits transfer of electrons from complex I to ubiquinone.
Isoflavone in roots and seeds of some plants
What does oligomycin do?
Antibiotic produced by streptomyces that inhibits oxidative phosphorylation by binding to stalk of ATP synthase and blocking the flow of protons through it
What is the role of dinitrophenol (DNP)?
Proton ionophore which shuttles protons across the inner mitochondrial membranes, reducing the proton gradient
uncouples oxidative phosphorylation from ATP production and increases metabolic rate and body temp
What are the advantages/disadvantages of DNP?
A - Used in pesticydes and food dye
D - as it increases metabolic rate it is in weight loss medication for obesity, however diff between poisoning or killing is slight so it is now illegal
