Cell Fractionation Flashcards
1
Q
What is the first stage of fractionation
A
Break cells open
2
Q
What is the temperature cells need to be at during fractionation
A
Ice cold
3
Q
Why do cells need to be kept cold during fractionation
A
Prevents enzyme activity
4
Q
Why do cells need to be isotonic when broken open
A
The same concentration
Prevents osmosis
Organelles don’t change size
5
Q
What is the second step to fractionation
A
Solution is filtered
6
Q
Why does the solution need to be filtered
A
Remove cell debris and whole cells
7
Q
What is the third step of fractionation
A
Ultracentrifugation
8
Q
What does ultracentrifugation do
A
Heaviest organelles compressed into pellet at bottom
9
Q
What is the heaviest organelle
A
Nucleus
10
Q
What is the order of organelles
A
(Heaviest) Nuclei Chloroplasts Mitochondria Lysosomes Endoplasmic reticulum Ribosomes (Lightest)
11
Q
How is a buffer used
A
Keep pH the same
