cell fractionation

Question 1

cell fractionation is…..

Answer 1

is the combination of various methods to separate cell organelle and components based upon size and density

Question 2

importance of cell fractionation

Answer 2

cell fractionation is useful for electron microscopy of cell components

Question 3

the principal of cell fractionation consists of two steps that are…

Answer 3

1. homogenization

2. centrifugation

Question 4

homogenization is…..

Answer 4

it is the formation of a homogeneous mass of cells

Question 5

in plants what is added to digest middle lamella?

Answer 5

enzyme pectinase

Question 6

centrifugation is the….

Answer 6

is the process of separating substances on the basis of their sizes and densities under the influence of centrifugal force

Question 7

centrifuge?

Answer 7

it is the machine by which centrifugation is done…

Question 8

two major ways of centrifugation….

Answer 8

1. density gradient centrifugation

2. differential centrifugation

Question 9

pellet

Answer 9

in differential centrifugation ,at each step the content which settles at the bottom are called pellet

Question 10

those contents that remain suspended above in the form of liquid are called….

Answer 10

SUPERNATANT