Cell Division, Cell Diversity and Cellular Organisation Flashcards
Cell Cycle: What is the cell cycle?
The cell cycle is the process that all body cells from multicellular organisms use to grow and divide. It starts when a cell has been produced by cell division and ends with the cell dividing to produce two identical cells. The cell cycle consists of a period of cell growth and DNA replication, called interphase, a period of cell division called mitosis.
Cell Cycle: What are the stages of the cell cycle?
- Mitosis
- Interphase
- Gap phase 1
- Synthesis
- Gap phase 2
Cell Cycle: What is the mitosis stage?
The cycle starts and ends here and is when the nucleus divides and chromatids separate, after this is cytokinesis, when the cytoplasm divides or cleaves.
-Mitosis only occupies a small percentage of the cell cycle and the remaining percentage includes the copying and checking of genetic information. as well as processes associated with growth.
Cell Cycle: What is interphase?
During interphase, the cell carries out normal functions, but also prepares to divide. The cell’s DNA is unravelled and replicated, to double its genetic content. The organelles are also replicated so it has spare ones and its ATP content is increased.
Cell Cycle: What is the G1 phase?
Gap phase 1: Biosynthesis - cell grows, organelles replicate and new organelles and proteins are made
Cell Cycle: What is the S phase?
Synthesis: cell replicates its DNA and checks the DNA for errors
Cell Cycle: What is the G2 phase?
Gap phase 2: cell keeps growing and proteins needed for cell division are made
Cell Cycle: How long does the cell cycle take?
The length of time for a parent cell to divide into two daughter cells, and for each to grow to full size, varies between species and cell type. It is also affected by the availability of nutrients for the cells.
-Some prokaryotic cells can go through the whole process in around 30 minutes, whereas yeast cells (single-celled eukaryotes) take about 4 hours.
Where is DNA held?
Chromosomes are in the nucleus of eukaryotic cells. Each chromosome contains one molecule of DNA, which includes specific lengths of DNA called genes. So the chromosomes that hold the instructions are sometimes called the blueprint, for making new cells. The daughter cells produced during the cell cycle mist contain a copy of all of these instructions s they must contain a full set of chromosomes, copied exactly from the chromosomes in the parent cell.
How many chromosomes do humans have?
In humans we have 23 different types of chromosomes. There are two copies in each cell so we have 46 chromosomes in the nucleus of each cell.
-Different chromosomes have different genes on them, there are 2 copies of each gene.
What is the structure of chromosomes?
The DNA double helix is wrapped around histone proteins, this makes nucleosomes. As this continues wrapping up, it produces chromatin which eventually makes chromosomes.
What is a histone protein?
Histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes.
What is a nucleosome?
Nucleosomes are the basic unit of DNA packaging in eukaryotes (cells with a nucleus), consisting of a segment of DNA wound around a histone protein core.
What is chromatin?
The material of which the chromosomes of eukaryotic organisms are composed, consisting of protein, RNA, and DNA.
-Material staining dark red in the nucleus during interphase of mitosis and meisois. It consists if nucleic acids and proteins. Chromatin condenses into chromosomes when supercoiled during prophase of cell division.
What is the definition of a chromosome?
A linear DNA molecule wrapped around histone proteins found in the nucleus.
What must happen before a cell can divide?
In eukaryotes, the molecules of DNA that make up each chromosome are wrapped around proteins called histones. The DNA and the histone proteins together are called chromatin. Before a cell can divide to produce two new daughter cells, the DNA of each chromosomes must be replicated. Two replicas are produced. Each is an exact copy of the original, and they remain held together at a point called the centromere. This plays an important role an the process of nuclear division.
What is produced when a cell’s DNA is replicated?
At this stage you can’t see the chromosomes under a light microscope. Each chromosome now consists of two replica DNA strands. These replicas are called a pair of sister chromatids. When they are separated from each other, each one will end up in a different new daughter cell.
What must happen to chromatin before it can be divided?
Chromatin must be coiled up (supercoiled) to form visible chromosomes. Each one is then short and sturdy enough the be moved around more easily. Supercoiled chromosomes can’t perform their normal functions in the cell, so the length of time they spend coiled up needs to be as short as possible.
How thick is chromatin and chromosomes?
Chromatin threads are about 30nm thick.
-After supercoiling a chromosome is about 500nm thick.
Why are chromosomes often shown as sister chromatids?
Single chromosomes would be too small to be seen with a light microscope.When copied chromatin supercoils and condenses, it becomes denser and more easier to stain and so can be seen easier with a microscope.
What is the advantage of supercoiling?
- Easier to move
- More compact
What happens as the chromosomes are being replicated?
As chromosomes are being replicated, proof-reading enzymes move along the new DNA strands and check that the copying has been done properly. If the genes are not copied precisely, the resulting mutations may mean the cells fail to function, and could become cancerous.
What other than a full set of chromosomes do daughter cells need?
Each new cell, like its parent, must carry out a number of metabolic function. In order to survive, it needs its own membranes, cytoplasm, organelles, enzymes and other proteins.
Why do all organisms need to produce genetically identical daughter cells?
- Asexual reproduction: single-celled organisms, such as Paramecium, divide to produce two daughter cells that are separate organisms. Some multicellular organisms, such as Hydra, produce offspring from parts of the parent.
- Growth: multicellular organisms grow by producing new extra cells. Each new cell is genetically identical to the parent cells and so can perform the same function.
- Repair: damages cells need to be replaced by new ones that perform the same function and so need to be identical.
- Replacement: red blood cells and skin cells are replaced by new ones.
Mitosis: What is mitosis?
Mitosis refers to the process of nuclear division where two genetically identical nuclei are formed from one parent cell nucleus, eventually forming diploid cells. It is a continuous process but is divided into four named stages.
Mitosis: What is the structure of a chromosome in mitosis?
As mitosis begins, the chromosomes are made of two strands joined in the middle by a centromere. The separate strands are called chromatids. Two strands on the same centrimere are called sister chromatids. There are two strands because each chromosome has already mad an identical copy of itself during interphase.
-When mitosis is over, the chromatids end up as one-strand chromosomes in the new daughter cells.
Mitosis: What are the four stages of mitosis?
-Prophase
-Metaphase
-Anaphase
-Telophase
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Mitosis: What happens in prophase?
- Each chromosome has already replicated in interphase. In prophase, the chromosomes condense (due to supercoiling) and become shorter and fatter. It can be seen that they consist of a pair of sister chromatids when using a light microscope at this point.
- At this time, the nuclear envelope breaks down and disappears, allowing the chromatids to move around the cell.
- An organelle called the centriole (a tiny bundle of proteins) divides in two, and each daughter centriole moves to opposite ends (poles) of the cell to form the spindle, a structure made of protein fibres.
Mitosis: What happens in metaphase?
The chromosomes (each with two chromatids) move to the middle of the cell, the central region of the spindle (the equator). Each becomes attached to a spindle thread by its centromere.
Mitosis: What happens in anaphase?
- The replicated sister chromatids that make up the chromosome are separated from each other when the centromere that holds them together splits. At this point, each of the ‘sisters’ effectively becomes an individual chromosome. Each one is identical to the original chromosome in the parent cell from which it was copied.
- The spindle fibres shorten, pulling the sister chromatids further and further away from each other towards the poles. They assume a V-shape because the centromeres, attached to the spindle fibres lead.
Mitosis: What happens in telophase?
- As the separated sister chromatids reach the poles, the spindle breaks down and disappears. The chromatids uncoil and become long and thin again, back into chromosomes.
- A nuclear envelope forms around each groups of chromosomes, so there are now two nuclei.
Mitosis: What happens in cytokinesis?
Cytokinesis is the division of the cytoplasm. The cell membrane constricts, pinching the cell into the two daughter cells. Each contains a full set of chromosomes identical to that found in the original parent cell.
-Having identical genetic information, in the form of identical chromosomes, means each daughter cell is capable of doing everything the parent cell could do.
Mitosis: Why is cytokinesis different in animal and plant cells?
- In plants, only meristem cells can carry out mitosis and cytokinesis. Plant cells lack centrioles to produce the tubulin spindles, these just form in the cytoplasm instead.
- In animal cells, cytokinesis starts sat the edge of as cell where the cell membrane constricts. This is not what happens in plant cells (due to the cell wall).
Mitosis: How does cytokinesis occur in plant cells?
Because plants have a cell wall, cytokinesis is different in plant cells to animal cells. It begins in the centre of a cell wall with a cell plate. This is a double membrane which secretes materials needed to make two cell walls (one for each daughter cells). The cell walls begin to form in the centre and move outwards until they meet the perimeter.
Mitosis: How does cytokinesis occur in yeast?
Cytokinesis in yeast involves budding of new cells of the original.