Cell Death

Question 1

Whats characteristic of apoptosis

Answer 1

active, shrinkage, intact membrane, dna fragments, organized death

Question 2

how do you get a DNA fragment for apoptosis? which enzymes and inhibitors are involved

Answer 2

caspase destroys ICAD inhibitor>CAD (caspase activated DNA-ase) can cleave DNA

Question 3

what are the 2 ways to activate initiatior caspases (bring them into complexes)

Answer 3

extrinsinc pathway-death receptor pathway intrinsic pathway-mitochondrial pathway

Question 4

Whats Extrinsic pathway

Answer 4

Fas ligand binds to fas death receptor that trimerize forming complexes >apoptosis

Question 5

What is the intrinsic pathway/mitochondria?

Answer 5

(stim by BH3, inhibited by bcl2)

> cytochrome C>apf1>caspases!

Question 6

What does IAP do? whats an antagonist that in turn promotes cell death?

Answer 6

inhibits apoptisis (IAP duh). SMAC is an an anatagoist so it will promote cell death and cyctochome C release

Question 7

What does p53 induce to induce apoptosis?

Answer 7

Bh3

Question 8

What does TNF-alpha do?

Answer 8

TNF-alpha activates a necrosis pathway mediated by RIP1, RIP3 kinase, and MLKL, which forms pores on cellular membranes.