Cell Culture Flashcards
What does KCl do
Swells cells using osmotic potential.
Allows cells to spread
What does PHA do
It’s a mitogen that stimulates the lymphocytes to undergo cell division
Phytohaemagglutinin
What does thymidine excess do
It inhibits the uptake of deoxycytidine causing DNA synthesis to slow down.
It ‘holds’ cells in s phase
What’s a clastogen
Material that causes chromosomal breaks
What do you expose cultures to if you’re investigating fanconi anaemia
DEB, MMC
What do you expose cultures to if you’re investigating ataxia telangiectasia
X-irradiation or bleomycin
What do you expose cultures to if you’re investigating Bloom syndrome
BrdU followed by UV exposure
If investigating fragile sites what do you add
Antifolate, BrdU, distamycin A
What does Colemid do
Breaks down spindle fibers
