Cell Biology - Genetic Engineering Flashcards
genetic engineering
a technology which allows the artificial transfer of genes from one organism, such as a human, into the cells of another organism, such as bacteria
stage 1 genetic engineering
identify section of DNA that contains required gene from source chromosome
stage 2 genetic engineering
extract required gene using enzymes
stage 3 genetic engineering
extract plasmid from bacterial cell (the plasmid will act as a vector - it will transfer the gene from one organism to another)
stage 4 genetic engineering
insert required gene into bacterial plasmid using a different enzyme
stage 5 genetic engineering
insert plasmid into host cell
stage 6 genetic engineering
grow modified cells to produce a genetically modified (GM) organism