cc1- LABORATORY L1 Flashcards
Used to improve the quality of the sample and accelerate sample processing.
ADDITIVES
Order of draw:
blood culture (if ordered, will always come first)
yellow
light blue
red (glass)
red (plastic)
SST or gold
green
PST or light green
lavender
pink
gray
Fibrinogen and other clotting factors are present since it is not used up because of the presence of an anticoagulant.
PLASMA
AKA serum separator tube
GOLD TOP
no anticoagulant but contains silica particles as clot activator.
RED TOP (PLASTIC)
no anticoagulant and additive because the glass itself serves as a clot activator.
RED TOP (glass)
allows bacteria to grow so that they can be cultured.
Sodium Polyanethol Sulfonate (SPS)
separator gel that is present in GOLD TOP
- principle: upon centrifugation, the different specific gravity will
separate the components
thrixotropic gel
LIGHT BLUE 2 CONCENTRATIONS:
3.2% (0.105M) (preferred) and 3.8% (0.129M)
No anticoagulant but it has a separator gel
GOLD TOP
LIGHT BLUE TOP
- means that it binds to
calcium or forms calcium salts to remove calcium.
CHELATES CALCIUM
LIGHT BLUE
- Blood to anticoagulant ratio:
9:1
Preferred anticoagulant for CC; best for blood gas studies, electrolyte studies
HEPARIN
3 FORMS OF HEPARIN:
LITHIUM, SODIUM, AMMONIUM
is the anticoagulant naturally found in the body, thus, it has the
least effect on chemistry tests.
HEPARIN
AKA plasma separator
tube
LIGHT GREEN TOP
LAVENDER TOP: concentrations
- K2 EDTA or dipotassium EDTA (recommended because it preserves the cell morphology and provides stable microhematocrit result)
PLASTIC (SPRAY-DRIED)
LAVENDER TOP: concentrations
- K3 EDTA or tripotassium EDTA (commercial name: sequestrene)
GLASS (LIQUID)
What is the commercial name of K3 EDTA
SEQUESTRENE
What is the commercial name of NA2 EDTA or DISODIUM EDTA?
VERSENE
is the most potent clot activator; used in STAT chemistries because
the optimum clotting time is 5-10 mins.
THROMBIN
Serum/plasma that contains large amounts of bilirubin
ICTERIC SAMPLE
Serum/plasma contains large amounts of fats and lipids
LIPEMIC SAMPLE
3 MAJOR TYPES OF THERMOMETER
- LIQUID-IN-GLASS
- ELECTRONIC THERMOMETER
- DIGITAL THERMOMETER
- Most COMMON type for volumetric measurement
BOROSILICATE GLASS
is calibrated to hold
one exact volume of liquid (TC). The flask has a round, lower portion with flat bottom and a long, thin neck with an
VOLUMETRIC FLASK
– designed to hold
different volumes rather than one exact amount
ERLENMEYEK FLASK
is determined in the same manner regardless of whether w/w, v/v, or w/v units are used.
PERCENT SOLUTION
defined as the number of gram equivalent weights per 1 L of solution.
NORMALITY
Expressed as the number of moles per 1 L of solution.
MOLARITY
MODE OF ACTION : OXALATE
Oxalate prevents cutting by binding
calcium
MODE OF ACTION :SODIUM FLUORIDE
Sodium fluoride preserves glucose and inhibits bacterial growth
MODE OF ACTION: 3.2 % buffered sodium citrate
Prevent coagulation by chelating calcium
represents the ratio of concentrated or stock material to the volume or weight of the concentrate plus the volume of
the diluent, with the units remaining the
same.
DILUTION
MODE OF ACTION: Silicon in RED TOPPED TUBE
Promotes Blood Clot
MODE OF ACTION: SPS
Prevent coagulation by binding calcium
MODE OF ACTION: Lithium Heparin
It inhibits thrombin formation to prevent clotting
