CC Flashcards
Total CO2 content in arterial blood.
19 to 25 mmol/L
In which condition is persistent hypoglycemia seen?
Insulinoma & Galactosemia
Sodium in serum is normally with in:
136 to 142 mmol/L (135 to 150 mmol/L)
Used as substrate for lipase activity
Either olive oil or trioline
Respiratory acidosis includes the following conditions:
Hypercapnia
Decrease in pH
Increase in pCO2
Increase of HCO3 as compensatory mechanism
RA - ⬇️ pH; ⬆️ CO2, HCO3
What is the normal partial pressure of CO2 in arterial blood?
38 to 42 mmHg
What effect is known as the affinity of hemoglobin for oxygen as affected by pH
Bohr’s effect
Tungsten blue product from reduction of phosphotungstic acid by uric acid is measured spectrophotometrically at wave length of:
650 to 700 nm
What is considered as the reference method for lipoprotein assay?
Ultracenfugation
A hypothetical true value of glucose in a specimen is taken at 5.0 mmol/L. Which analytical value shows accuracy of the test assay?
5.0 mmol/L
Using the +/- SD, a technologist is able to decide whether to reject or accept the results of a particular run being in or out of control, with a certainty factors:
95%
In which part of the nephron does reabsorption of water, important electrolytes and metabolites primarily occur?
Proximal tubules
Dry chemical extinguisher can be used to put off fire caused by the following, EXCEPT:
❎ Combustible chemical and metals (D)
✅ Flammable solvents, alcohol, gas (B)
✅ Electrical malfunction as in workings or in equipment (C)
✅ Paper and wood (A)
A - Water
ABC - DC
BC - CO2, Halon
Cretinism in infants is caused by:
Hypothyroidism
What is the function of pyridoxal-5-phosphate in the reactions catalyze by the aminotransferases?
Prosthetic group
When centrifuging test tubes with serum separator gel, which of the following instruments must be used?
Swinging bucket type centrifuge
Which of the following for LDL cholesterol is taken as desirable?
125 mg/dL
Condensation reaction of glucose with orthodo-toluidine in hot acidic condition results in final _______________ colored product.
Green 💚
In what method is p-nitrophenylphosphate used as a substrate for ALP assay?
Bessy-Lowry-Brock and Bowers McComb
The process of separating serum proteins into components by virtue of their net charges in an electric field is known as:
Electrophoresis
Bromcresol purple is used in the dye-binding method for:
Albumin
Repeated and vigorous fist exercise during venipuncture falsely increases the concentration of:
Potassium (K+)
Which of these are necessary for a patient to undergo oral glucose tolerance test?
✅ Diet of at least 150g CHO/day for 3 days
✅ Overnight fasting prior to the test
✅ No strenuous physical activity, smoking nor drinking during the test
What is the major precursor substance for the production of bilirubin?
Hemoglobin released from aged blood cells
Which of the following parameters are decreased in primary hypothyroidism?
T4, T3, Resin T3 uptake (Decreased)
*Increased TSH
A serum sample shows a chloride concentration of 475 mg per 100 mL, which is the same as:
MW of Cl: 35.5
133.8 mEq
(475/35.5)/0.1L
In the event of myocardial infarction, which of these enzymes becomes the FIRST elevated in serum?
Creative Kinase
- CK
- AST
- LDH
What is the molarity of a 10 N sulfuric acid solution?
5
N = VM 10 = 2M M = 5
The following are analytical values for Calcium in mg/dL; determine the mean.
- 5
- 5
- 5
- 4
- 8
- 7
- 0
- 6
- 3
- 0
10.1
In adult, hypoglycemia is defined by a plasma glucose level of:
< 50 mg/dL
Measurement of blood pH and gasses is routinely performed at what temperature?
37 deg Celsiues
What is the substrate used in the Bessey-Lowry-Brock method for ALP assay?
P-nitrophenyl phosphate
Majority of the thyroid hormones in blood are bound with:
Thyroxine binding globulin (TBG)
In order for iron absorbed from the small intestines to be carried in the plasma, it has to be bond by a carrier substance. Which of the following serves this function?
Transferrin
In HDN, what type of bilirubin is expected to be elevated?
Indirect bilirubin
Abnormal levels of this electrolyte show very characteristic changes in ECG tracings. Which one is it?
Calcium
Ca, K, Mg
What reaction is used in the Wroblewski-La Due method?
Pyruvate to lactate catalyzed by LDH
WLD - Reverse method for LDH
The reagent caffeine sodium benzoate in the Jendrassim Grof method serves for:
Accelerator reagent
Majority of plasma thyroxine (T4) is:
Bound to globulin
Tophi that deposit on soft tissue and joints consists of:
Sodium urate
Which of these antibodies are assoc. with autoimmune thyroiditis?
Both microsomal and thyroglobulin antibodies
Total bilirubin of an adult female is 12 umol/L and direct bilirubin of about 4 umol/L. The indirect bilirubin is expected to be:
Within normal range
An enzymatic method used to analyze ethanol measures NADPH from the oxidation of ethanol + NADP. What enzyme catalyzed this reaction?
Alcohol dehydrogenase
Amylase isormzyme fractionation assay makes used of wheat germ-derived enzyme, which serves to inhibit:
Salivary amylase
One of the following is not secreted by the anterior pituitary gland (APG). Which one is it?
❎ VMA
✅ LH
✅ HGH
✅ ACTH
Which of the following forms of exposure places a MT at the highest risk of infection with HIV?
Needle stick from AID contaminated needle
What enzyme catalyzed the hydrolysis of urea to ammonium ions and bicarbonate?
Urease
Given the ff. data, determine the carbonic acid level: pH = 7.07 HCO3 = 8.3 mmol/L pCO2 = 29.7 mmHg Delta content = -22.6
0.9 mmol/L
H2CO3 = DCO2
DCO2 = pCO2 x 0.031
DCO2 = 29.7 x 0.031
DCO2
Hemolysis of serum causes false increase in creatine kinase activity because:
Adenylate kinase profuse more ATP or ADP
This characterizes the pattern of secretion of creatine kinase in acute myocardial infarction
It starts to rise in 6-8hrs after onset, reaches peak in 6-18hrs and returns to normal in 3 days
Alkaline phosphatase is increased in both hepatobiliary and bone diseases. (This statement would help make a differential dx)
LAP, GGT, and 5’N are increased in hepatobiliary disease ONLY
What biochemical substance characterizes as being insoluble in water and in most biological fluid
Triglycerides