CBG 33

Question 1

nuclear lamina , role? interactions? connectd to?

Answer 1

meshwork of lamin proteins
gives nucleus shape and stability
interacts with a)proteins of inne membrane
b)chromatin

note- it is dissassembld during mitosis

it is connected to the inner nuclear membran chromatin and chromatin proteins

Question 2

Nuclear pore complex, size?structure?role?

Answer 2

ver big 25Mda, 100/200nm in diameter
30 different nucleoporins proteins and many copies. mplex!
structure = cytoplasmic fibrils,nuclear basket,central framework (contains FG reeat cores)

role=Permeability barier

Question 3

how to visualise NPC?

Answer 3

Cryo-electron tomography

Question 4

how was the architecture of the NPC derived?

Answer 4

1) crosslinking (show was anchored to nuclear envlope)
2) mass spec/proteomics (to identify dif proteins)
3) immuno/EM localisation (where?)
4) analytical ultracentrifugation-size + shae

Question 5

RER

Answer 5

closest to nucleus,makes membrane proteins

Question 6

SER roles

Answer 6

lipid/cab metabolism, protein mod and isolates Ca+ ions away from cytosol

Question 7

difference between er and golgi body?

Answer 7

er=inteconncted tubes branching from nuclear envelope

golgi-ordered series of compartments called cisternae

Question 8

how is the golgi body suported mchanically?

Answer 8

integrity=microtubules and cytoskeleton

organising stacks-golgi matric proteins

Question 9

golgi body function

Answer 9

soting and dispatching oteins
carb synthesis
micotubule nucleation

Question 10

TGN?

Answer 10

trans golgi network- where proteins are packaged

are adjacent to stacks o golgi apparatus but can b separate

Question 11

CGN?

Answer 11

cis golgi network, bud off er

Question 12

what can each cisternal sac have?

Answer 12

a trans ENTRY face or a CIS Exit face

Question 13

golgins

Answer 13

peripheral proteins on the cytoplasmic side
200mm filaments
coiled-coiled proteins that aid communication between sacs
ae diverse binding proteins

Question 14

peroxisomes

Answer 14

Enclosed by a lipid bilayer
More or less spherical organelle
Important for oxidative reactions (energy metabolism)
Breakdown long chain fatty acids

Question 15

lysosomes

Answer 15

Enclosed by a lipid bilayer
More or less spherical organelle, vary in size, identiied as dense 
Low pH (5)
Waste disposal center:
Proteins
Nucleic acids
Carbohydrates
Lipids
debris

Question 16

endosomes

Answer 16

Enclosed by a lipid bilayer
Spherical, vary in size
Three main types:
Early (Sorting) 
Recycling (return to PM)
Late (target for degradation e.g lysozome which is a type of endosome)