CBC 2 and Other Routine Procedures Flashcards
The red iron- bearing protein contained within the
erythrocytes in normal blood.
Hemoglobin (Hb)
Bone Marrow is unable to produce RBC
Aplastic Anemia
The first technique is used for Mass Blood Donation, the Second technique is routinely used.
- Copper Sulfate 2.Colorimetric Methods
If the blood sinks, the level of hemoglobin is?
Acceptable (>12g/dl)
It is the former routine method where blood is lysed with Saponin
Gasometric Method or Oxygen Capacity Method
In the Gasometric Method, what apparatus is used to collect and measure gas?
Van Slyke apparatus
What is the specific gravity of blood?
1.053
What method is used for Mass Blood Donation?
Copper Sulfate or Specific Gravity Method
How much is the iron content of Hgb?
1000mg contains 3.47 iron
What is a direct/visual method that uses a simple chart with varying hues of red color for comparison with a 30-50% error percentage?
Talquist Scale
What is a direct/visual method that uses a glass plate and eyepiece with a 20-30% error percentage?
Dare Hemoglobinometer
What is a direct/visual method that uses an acid solution to lyse RBC and liberate Hgb. After 10 mins, drops of water are placed until it is the same color with a comparative block.
Acid Hematin
What is a direct/visual method that uses an alkaline solution for Hgb determination and uses heat to lyse the RBC?
Alkali Hematin
What is an indirect method that uses photometry?
Oxyhemoglobin
What is an indirect photometric method that is simple and quick but has no possibility of preparing a stable HbO2 standard?
Sodium Carbonate
What is an indirect photometric method that measures pulse oxygen saturation and pulse rate through the finger?
Photoelectric Oxyhemoglobin
What is an indirect photometric method that is done only when carbon monoxide poisoning is suspected?
Carboxyhemoglobin
This technique allows rapid estimations of large number of red cells by imposing two squares (one square is nine times the area of the other square)
Calibrated Miller Disk
How many Red Cells are counted in successive microscopic fields in Calibrated Miller Disk
300 Red Cells
Techniques in Dry Method (Reticulocyte count)
New Methylene Blue, Cook, Mayer, Tureen, Seiverd’s
A microscopic method that uses a film of stain is spread on a glass slide and a drop of blood is added. The preparation is covered with a coverslip.
Reticulocyte Count Wet Method
Reticulocyte Count Wet Method is counted using:
Light Microscope or Calibrated Miller Disk
Optical aid inserted into the eyepiece of the
microscope. This allows for more accurate count.
Miller Disc Method
Area of the secondary square in comparison to the larger square (Miller Disc)
1/9
Automated reticulocyte analyzer which uses
flow cytometry for the determination of percent reticulocyte as well as the absolute reticulocyte count.
Sysmex R-1000
Increase in reticulocytes in blood are caused by:
Anemia, poisoning, infestations, intoxications, leukemia, and Kala-azar
Decrease in reticulocytes are caused by:
Aplastic Anemia, Chronic Infections
Classically employed as an index of the presence of active diseases like tuberculosis, tonsillitis, rheumatic fever, and rheumatic heart disease
Erythrocyte Sedimentation Rate
The test depends on the fact that in the blood to which anticoagulant has been added, the red corpuscles sediment until they form a packed
column in the lower part of the tube or container
Erythrocyte Sedimentation Rate
- It is used as an index of the presence of an active infection.
- It measures the suspension stability of RBCs.
- It indicates abnormal concentration of fibrinogen, globulin, and other
plasma proteins.
Erythrocyte Sedimentation Rate
Most preferred ESR Method and it is the most sensitive for chronic diseases.
Westergren Method
Anticoagulant of Wintrobe-Landsberg and Westergren
Double Oxalate / 3.8% Trisodium Citrate
