CBC Flashcards
Hemogram/CBC components
RBC
Hemoglobin - number
Hematocrit - percentage of blood containing Hb
Indicies (MCV**, MCH, MCHC)
WBC count -differentials
- Neutrophils
- Lymphocytes
- Monocytes
- Eosinophils
- Basophils
Platelets count
anemia: main DX parameter + signs and sx
Main parameter to dx anemia: LOW HEMOGLOBIN **
Signs and symptoms of anemia:
- fatigue
- pale (nails and conjunctiva)
- tachycardia + tachypnea: physical compensation
- hypotension
- chest pain (rarely)
Physical compensation of RBC anemia
Tachycardia + increase RR
… Long Term–> Hypertrophy –> Heart needs more O2–> Body doesnt have–> Heart Failure
Production compensation of RBC anemia
Kidneys and liver increase EPO release which then increases reticulocytes and RDW
RBC definition
-mature RBC in blood
hmg
-iron containing protein found in RBC
-carriers of oxygen and CO2
Hematocrit (Hct) definition and what causes low/high Hct levels
Definition: measures the proportion of RBCs in the blood
- expressed as a percentage or ratio of volume of RBCs relative to total blood volume
- ex: 40% HCT = 40 mL of red blood cells in 100 mL of blood
-Hct is approx 3x hmg
-direct relationship with hmg
Low HCT:
- anemia
High HCT with normal HMG:
- dehydration
High HCT with high HMG:
- high altitude
- pulmonary disease
- polycythemia vera
Effect of IV Fluids on Hematocrit and Hemoglobin
Administering intravenous (IV) fluids can lead to a dilution of blood component
- HCT: DECREASES because of increased plasma volume
- Hemoglobin: unchanged
Mean Corpuscular hemoglobin concentration(MCHC):
- Assesses the CONCENTRATION of hemoglobin + size (? - idk her slides says it does)
- indicates how dense the hemoglobin is within RBCs: COLOR
- Hb/Hct
- Hypochromic: pale color bc not dense
- normochromic
- hyperchromic: very dense
Think using watercolor paint: more concentrated = higher pigment/color
Mean Corpuscular Hemoglobin(MCH):
- WEIGHT of hemoglobin in the RBC
-Hgb/RBC count
Mean Corpuscular Volume (MCV)
aka the QUALITY of RBCs, dense or not dense enough?
- Hct/RBC
- checks the SIZE or VOLUME of RBC
- helps to classify TYPES of anemia
Low MCV: microcytic
Normal MCV: normocytic
High MCV: macrocytic
“the only one that doesnt have Hb in the name -> numerator is HCT”
types of anemia + which parameter
-normocytic
-microcytic
-macrocytic
-defined by MCV
red cell distribution weight (RDW)
Measurement of the RANGE in the volume and size of your RBC
- aka: the “precision” of the RBC
-when all the RBCs are similar -> RDW is normal
-when some RBCs are small or some large -> RDW is elevated
-“the poor mans reticulocyte count”- if reticulocytes are high RDW is high (new cells measure bigger) -> compensating for anemia
-if someone is anemic and RDW is low -> why arnt they compensating?
reticulocyte count + reticulocyte index
reticulocyte count = # of immatures RBC
-normally only 0.5-1.5%
-90-120 days life span of RBC
-high reticulocyte count: indicates effective bone marrow response to anemia
- low reticulocyte count: indicates inadequate production of RBCs
Reticulocyte index: corrected count that takes into account degree of anemia + reticulocyte lifespan
Less than 2%: decreased production problem
Greater than 2%: increased destruction or blood loss
- bleeding
-hemolysis
peripheral blood smear
-number, shape, and appearance
- sickle cell: look at peripheral blood smear
serum iron + components of RBC
-essential component of hemoglobin
-lack of iron -> cant build hemoglobin
components of RBC:
-folic acid
-b12
-iron
shilling test
B12 deficiency testing*
-Radio labeled B12 given orally
-Urine collected 24 hrs, checked for B12 level
-normal: 8-40% should be excreted
-If less with dose of intrinsic factor then indicative of pernicious anemia
-B12 deficiency- macrocytic anemia
coomb’s test
-used to detect ANTIBODIES that act AGAINST the surface of your RBC
-autoimmune destruction of RBCs -> hemolytic anemia
-presence of these ANTIBODIES = immune
hemolytic anemia
immune hemolytic anemia:
- blood does not contain enough RBC because they are destroyed prematurely
- presence of antibodies
-possible jaundice
“Honey COOMBs = yellow = jaundice”
anemia diff dx: Proliferation (Can’t produce!)
1) Anemia of CHRONIC disease: chronic inflammation ds
- Rheumatoid Arthritis
- lupus
- chronic infections
- malignancy
-bone marrow not as active to produce RBC or EPO deficient
2) Renal disease- Deficient EPO
3) Aplastic anemia: insuffient bone marrow
- Fanconi anemia: Inherited aplastic anemia
- Blackfan-Diamond syndrome: Inherited bone marrow failure
- Parvovirus infection causing aplastic anemia (bone marrow can be suppressed) -> temporary
4) Drug or toxins
“PRO-LIFE” - “CLIMB FDR Paranoia”
- Prolife: proliferation
- Chronic diseases
- Lupus/ rheumatoid arthritis (autoimmune)
- Infections (chronic)
- malignancy
- Blackfan-Diamond syndrome
- Fanconi anemia
- Drugs/Toxins
- Renal disease
- Parvovirus infection
anemia diff dx: maturation (trouble assembling)
Vitamin deficiencies:
- Vitamin B12 deficiency: Pernicious anemia
- Folate deficiency- normo or macro
- Iron deficiency- normo or micro
Sideroblastic:
- Iron in the mitochondria has abnormal sequestration
-ringed sideroblasts
Lead poisoning
- Inhibited heme synthesis
- decreased survival of RBC
“if you Mature = LIFe SaVe -> as you mature you get older and your life is save+ have a better diet - vitamins, iron, folate”
-Maturation anemia ddx
-Lead poisoining
-Iron deficiency
-Folate deficiency
-Sideroblastic
-Vitamin B12 deficiency
anemia diff dx: hemolysis (broken down!)
-Hemoglobinopathies- spleen kills RBCs due to poor quality RBCs
-Immune hemolytic anemia- autoimmune
-Infectious causes of hemolysis
-Membrane abnormalities
-Metabolic abnormalities
-Mechanical hemolysis: valve replacements
-Drug or toxins
-Wilson disease
- Copper in RBC, cell damage -> Kayser-Fleischer rings on eyes
“3 Ms 2Is DW H” -> Don’t Worry be Happy -> think dont break down (hemolysis) -> be happy! (reach lol)
- Metabolic abnormalities
- Membrane abnormalities
- Mechanical hemolysis: valves
- Immune hemolytic anemia
- Infectious causes
-Drugs
- Wilson
- Hemoglobinopathies
anemia diff dx: hemorrhage (disappearing act!)
-hypersplenism: overactive spleen -> excess destruction of RBCs
-acute/chronic hemorrhages: bleeding
microcytic dx
Microcytic: MCV < 80 fL
-MC: iron deficiency ***
Normal reticulocyte:
- iron deficiency ***
-Sideroblastic
-Anemia of Chronic Disease
-Thalassemia
High reticulocyte: (body is trying to compensate)
-immune hemolytic
-hereditary spherocytosis- inherited RBC disorder -> loss of biconcave shape, lack central pallor
-membrane or enzyme defects
“In Small Anemias, Cells Tire + High HIME”
- Iron deficiency
- Sideroblastic
- microcystic anemias: normal reticulocytes
- Chronic diseases
- Thalassemia
High HIME: high reticulocyte
- Hereditary spherocytosis
- immune hemolytic
- membrane defect
- enzyme defect
Think of the cells tiring because of their high hime (princess) -> cells at work vibes