Categorised parasites Flashcards

1
Q

Parvovirus

A

Non enveloped

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2
Q

Papillomaviridae

A

Non enveloped

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3
Q

Poxviridae

A

Enveloped

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4
Q

Staphylococcus

A

Gram pos cocci
Clusters
facultative anaerobes

Catalase +
Alpha haemolysis (incomplete)
Beta haemolysis (complete)

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5
Q

Which staph is coagulase positive?

A

S. aureus
S. pseudintremedius
S. hyicus (mostly)

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6
Q

Which staph is coagulase negative?

A

S. epidermidis
S. hyicus (some)

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7
Q

Streptococcus

A

Gram pos cocci
Chains
Facultative anaerobes
Catalase neg
Alpha haemolysis (incomplete)
Beta haemolosis (complete)

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8
Q

Which Strep have long chain morphology (broth)

A

S. agalactiae
S. equi subsp equi

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9
Q

Which Strep have short chain morphology (broth)

A

S. uberis
powdery deposit

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10
Q

Strangles biochemistry

A

lactose (neg)
sorbitol (neg)
Trehalose (neg)

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11
Q

Actinomycetales

A

Gram pos rods
Aerobes, microaerophilic and
anaerobes
Rhodococcus equi (only sig nif one)

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12
Q

Rhodococcus equi (Actinomycetales)

A

coccobacillus
strict aerobe
acid = fast
catalase pos
urease neg
glucose ferm neg
nitrate = nitrite
salmon pink SBA colonies
CAMP +

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13
Q

Corynebacterium

A

Gram pos small rod
coryneform
club shaped
catalase pos
non acid fast

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14
Q

C. pseufotuberculosis (Corynebacterium)

A

facultative anaerobes
CAMP +
sensitive to penicillin

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15
Q

C. renale (Corynebacterium)

A

CAMP +
non haemolytic

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16
Q

Actinomyces bovis

A

Catalase neg
penicillin sensitive

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17
Q

Features of B. anthracis

A

– Gram + rods
– Large blunt ended rods
– Chains of rods
– Endospore forming
– Aerobic condition
– Central endospores
– Capsulated
– Susceptible to penicillin
Capsule and exotoxins

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18
Q

Genus: Clostridium

A

– Large Gram + rods
– Catalase –
– Some are motile
– Flagella
– Endospore forming
– Spores survive adverse environments
– Strict anaerobes
– Microaerophilic
– Enrichment cultures
– Love niches with low oxidation-
reduction (redox) potential
* Canned meat !!!
* Bruised muscle !!

– Gas production
EXOTOXINS
– Saprophytes
– Gut commensals

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19
Q

Pseudomonas and Burkholderia genera

A

– Gram – rods
– Straight or slightly curved
– Medium sized
– Oxidase +
– Grow on MacConkey
– Strict aerobes
– Nitrate utilisation
– Motile
– Polar flagella
– Appearance and odour
– Widely distributed
– Soil and water
– Skin and mucous membranes

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20
Q

Order Chlamydiales

A

Aerosol spread

– Obligate intracellular Gram negative bacterium
– Have no peptidoglycan cell wall
* Trilaminar membrane containing lipopolysacchrides
and proteins
* Enveloped by disulphide cross-linked proteins
– Therefore stain poorly with Gram stain
– Prefer Giemsa (DiffQuik)
– Cannot grow them in the lab except in cell culture or
embryonated eggs
– Cell membrane and envelop proteins confer species and
type-specificity and may act as adhesins

21
Q

Moraxella

A

– Gram –
– Small, short rods
– Pairs
– Obligate aerobe
– Non motile
– Oxidase +
– Fastidious
– OBLIGATE PARASITE
– Mucous membranes
– Host specific

22
Q

Brucella

A

– Gram –
– Coccobacilli / short chains
– Strict aerobes
– up to 21 days to grow
– Non-motile
– Oxidase +
– Acid-fast staining
– Intracellular
– Reticuloendothelial system
– Adenine and guanine monophosphate
* Inhibit phagolysosomal fusion
* Inhibit myeloperoxidase-halide system
– OBLIGATE PARASITE
– Reproductive tract
– Biovars

23
Q

Foot rot

A

Fusobacterium necrophorum
* Gram negative rod
* Filamentous
* Obligate anaerobe
* Commensal of the alimentary tract and urogenital tract
– Associated with ‘lumpy jaw’ in kangaroos
* Found in the soil

Dichelobacter nodosus
* Previously known as Bacteroides nodosus
* Gram negative rod, large pleomorphic with terminal
enlargements (dumbbell shape)
* Obligate anaerobe
* OBLIGATE PARASITE of ruminant hooves
– The two work synergistically to cause foot rot

24
Q

Pasteurellaceae

A

– Gram negative small rods/coccobacilli
– Oxidase positive (mostly)
– Facultative anaerobes
– Non motile

Most commensals

25
Actinobacillus
– Gram – short rods/coccobacilli – Oxidase positive – Facultative anaerobes – Commensals – Oral – Respiratory tract – Reproductive trac *sticky colonies
26
Pasteurella and Mannheimia
– Gram – short rods/coccobacilli – Bipolar staining – Facultative anaerobes – Oxidase + – +/- Growth on MacConkey – Normal flora of the oral and upper respiratory tract
27
Salmonella
– Gram - ve, large rods – Oxidase – ve – Non-lactose fermenter – XLD agar – Most are motile – Worldwide, obligate parasite of the GIT
28
Genus Proteus
– Gram - ve, large rods, Oxidase – ve – Non lactose fermenter – VERY MOTILE !!! – Peritrichous (all over the cell) flagella – ‘Swarming’ colony morphology – Opportunistic pathogen – Urinary tract disease * Cystitis
29
Genus Yersinia
– Gram - ve, large rods, – Oxidase – ve – Bipolar or safety pin ends – Non motile (no H antigen) – Non lactose fermenter – Maintained in carriers
30
Enterobacteriaceae
– Oxidase negative – Large Gram-negative rods – Facultative anaerobes – Saprophytes and obligate parasites – All Grow on MacConkey Aga
31
Enterobacteriaceae lactose fermenters
– Escherichia – Klebsiella – Enterbacter – Citrobacter – Serratia
32
Enterobacteriaceae non lactose fermenters
– Salmonella – Yersinia – Proteus – Shigella
33
Escherichia coli
– Gram negative rod – Oxidase negative – Lactose fermenter – Usually, motile – Peritrichous (all over the cell) flagella – Coliform – Reside in the lower GIT
34
Genus : Listeria
– Gram + rods – Uniform shape – Widely distributed in nature – Commensals – Saprophytes – Wide temperature range
35
L. monocytogenes
– Gram + rods – Short regular – Facultative anaerobes – Motile – Catalase + – Hydrolyse aesculin – CAMP + – Narrow zone of b haemolysis – Psychrotrophic
36
Genus: Erysipelothrix
– Gram + short rods – Some form filaments – Non-motile – E. rhusiopathiae is the most important – Pigs – Septiacemia * Diamond skin disease – Zoonosis
37
Features of E. rhusiopathiae
– Gram + rods – Short regular (smooth) – Filament form (rough) * Bottle brush appearance in nutrient gelatin slabs – Facultative anaerobes – Catalase - – Pin point colonies – a haemolysis
38
Herpesviridae
-Large 200-250 nm -Icosahedral internal symmetry -dsDNA Abortion in horses (EHV1)
39
Family Picornaviridae
– Positive sense single stranded RNA – Go straight into proteins synthesis once in cell – Cycle of replication is FAST !! – Cytoplasm – Prone to frequent mutation (RNA viruses) – NON - ENVELOPED – STABLE IN THE ENVIRONMENT ! * Variation in their pH stability between viruses in the family * Only certain disinfectants are suitable for use against a given virus – Relatively heat stable * Especially if shielded by organic material – Cytolytic – Icosahedral Hand foot and mouth AEV notifiable disease --> PCR and ELIZA
40
Family Caliciviridae
– Small (25-40 mm in diameter) – Positive- sense single stranded RNA virus – +ssRNA virus – NON-ENVELOPED – Icosahedral symmetry – Stable in the environment – RESISTANT – Heat and detergents – Moderately pH stable * Inactivated under acidic conditions (pH<3) – Replicate rapidly in cytoplasm – Cell lysis = CYTOLYTIC RHDV
41
Family Coronaviridae
– Large (120-160 nm diameter) – Pleomorphic – Spherical, disc, kidney, rod-shape – Positive-sense single stranded RNA virus – +ssRNA virus – Genetic recombination between related coronavirus – Genetic diversity ✓ – ENVELOPED – Cytoplasmic replication – Can survive 3-4 weeks in the environment – Sensitive to heat, lipid solvents, detergents etc.
42
Family Flaviviridae
– Positive sense single stranded RNA – Replicate in the Cytoplasm * Released by exocytosis – ENVELOPED * Tightly adherent envelop associated with icosahedral capsids * Unstable (labile) in the environment * Easily inactivated by: – Heat – Common disinfectants JE WNV KUN BVDV
43
Family Togaviridae
Positive sense single stranded RNA – Replicate in the cytoplasm * Budding – ENVELOPED (‘Toga’ or gown) * Glycoprotein envelop associated with icosahedral capsids * Unstable (labile) in the environment * Easily inactivated by: – Heat – Common disinfectants – Lipid solvents EEE WEE VEE RR
44
Family Reoviridae
– Non-enveloped, icosahedral – Moderate resistant to heat, solvents and detergents – Double-strained RNA (dsRNA) – Replicates in the cytoplasm – Virions released by cell lysis – Segmented genome – 10-12 segments – Genetic re-assortment BT (orbivirus) rotavirus
45
Orthomyxoviridae
– Influenza viruses – Negative sense single stranded RNA (-ssRNA) – Replicate in the nucleus * Released from the cells by budding – ENVELOPED – Two major glycoproteins – Haemagglutinin (H or HA) * Allow virus to attach to respiratory epithelium * Bind RBC useful in diagnostic assays * Changes in H gene are very important * Greatest ANTIGENIC CONSEQUENCE – SHIFT (pandemics = worldwide) and DRIFT (epidemics = local populations) * 16 haemagglutinin types – Neuraminidase (N or NA) Very fragile Very prone to mutations INFLUENZA
46
Paramyxoviridae
– Negative sense single stranded RNA – Non segmented – Replicate in the cytoplasm * Released from the cells by budding – ENVELOPED – Two major glycoproteins ‘spikes’ – Attachment proteins * Haemagglutinin-Neuraminidase protein (HN) * Proteins without neuramindase activity (G) * Allow virus to bind to host cell – Fusion proteins (F) * Causes the virus envelop to fuse with the host cell – Pleomorphic shape – Glycoproteins DO not mutate as readily as influenza – Spread by respiratory or ocular discharge – Contagious – Very fragile in environment – Environmentally labile – Cell mediated immunity is important – Some produce cell- associated viraemia HENDRA Nipah
47
Rhabdoviridae
– Negative sense single stranded RNA – Non segmented genome – Replicate in the cytoplasm * Viral factory known as a Negri body – Cytoplasmic inclusion body * Released from the cells by budding – ENVELOPED – Glycoprotein spikes – G proteins * Interacts with host cell receptors to facilitate endocytosis of the virion * Induces virus-neutralising antibody – Cross-reactivity between some strains !! * Induces cell-mediated immunity – Fragile in the environment – Environmentally labile – Sensitive to light and drying – Survives well in dark places especially at low temperature * CAVES !!!!!! – Have a unique bullet shaped appearance RABIES BAT LYSSAVIRUS
48
Family Retroviridae
– Enveloped, +ssRNA – Diploid (two linear strands) – Icosahedral capsid – Budding * Acquires envelop from plasma membrane of host cell – Very labile (fragile) – Inactivated by lipid solvents, detergents, soaps, heating at 56⁰C for 30 minutes and drying. – Mutation and recombination occurs with high frequency. CAN CAUSE CANCER FIV MADCOWDISEASE