Catalase test

Question 1

Catalase is an enzyme used to break down

Answer 1

hydrogen peroxide

Question 2

Catalase test can be done directly on the

Answer 2

microorganism, but we will do it on a slide

Question 3

The catalase test is simple

Answer 3

Treat sample with hydrogen peroxide

If sample produces gas (i.e. it bubbles), it makes catalase

Question 4

The oxidase test determines if a microorganism can

Answer 4

perform aerobic respiration

Question 5

The oxidase test can be

Answer 5

done directly on the microorganism, but we will do it on filter paper

Question 6

The oxidase test is simple:

Answer 6

1. Treat the filter paper with p-aminodimethylaniline oxidase reagent
2. Spread sample on the treated paper
3. If sample turns purple, it produces oxidase