CASE 6 - HIV/HEPATITIS Flashcards
Name the 2 types of HIV and the patients they affect
HIV-1 worldwide pandemic, ~36 million people
HIV-2 West Africa/Europe, 1-2 million people, generally slower progression than HIV-1
Name the family and viral characteristics of HIV
FAMILY: retroviridae
CHARACTERISTICS: ssRNA transcribed to double-stranded DNA by reverse transcriptase
Name the antigen that is essential for HIV cell attachment
gp120
Describe the life cycle of HIV
- HIV gp120 binds to a CD4 receptor on CD4 cell AND a co-receptor
- Fusion and release of genetic material
- HIV ssRNA transcribed to dsDNA by reverse transcriptase enzyme
- Once it becomes dsDNA, it is integrated into the host by integrase
- This T cell/CD4 cell basically just becomes a factory for more and more HIV RNA through normal cellular processes
- HIV virion assembled and released
How is HIV transmitted?
- Mucous membranes/blood/tissue (below the skin) being exposed to INFECTED BODILY FLUIDS (e.g. blood, semen, vaginal fluid, breast milk)
- Mother to child transmission (particularly during delivery)
Sexual transmission most common
It is NOT transmitted through direct contact (e.g. hugging, kissing, sharing cutlery) or droplets or airborne
Name 5 factors that increase risk of HIV transmission
HIGH VIRAL LOAD in the source (most important)
Concurrent STI (e.g. syphilis) - mucous membranes exposed, easier for HIV to gain access
Bleeding or gential/rectal trauma
Drug use
More sexual partners
What type of immunity is most important when responding to HIV?
Cell-mediated
HIV subverts the immune system in the following ways:
- Infects CD4 cells that control normal immune response
- Integrates into host DNA
- High rate of mutation (moving target)
- Hides in ‘immune-privileged’ tissues
- Induces a cytokine environment that the virus uses to its own replication advantage
Explain how each strategy makes it more difficult for the immune system to fight it.
- Infects CD4 cells that control normal immune response: impairs immune function, facilitates opportunistic infections and the development of malignancies.
- Integrates into host DNA: difficult for immune system to recognise it.
- High rate of mutation (moving target): when HIV is recognised and wiped out, the other ones that have mutated and present a different antigen will NOT be targeted.
- Hides in ‘immune-privileged’ tissues: e.g. brain, bone marrow, reproductive organs where immune cells don’t routinely enter.
- Induces a cytokine environment that the virus uses to its own replication advantage: changes the regulation of the immune system by invading CD4 cells, using it to replicate themselves.
The precipitous, massive, and concealed depletion of CD4 cells causes impaired ability to…
(2 things)
- Recognise new infections/antigens
- Maintain immune memory responses (i.e. old infections that the host once developed immunity against will still infect them)
Looking at a graph of the natural history of HIV, explain what is being shown by the two different lines
(google an image)
- Depletion of CD4 cells in the first 4 weeks
- Acute HIV syndrome as large-scale dissemination of the virus occurs
A small recovery of the CD4 count corresponds with a period of clinical latency (lasts anywhere from a few years to decades) as the viral load drops.
CRITICAL POINT REACHED: CD4 count becomes low enough that the person develops infections and malignancies
An estimated __ to __% of people remain asymptomatic / unaware that they have contracted HIV
An estimated 10 to 60% of people remain asymptomatic / unaware that they have contracted HIV
Are there any specific HIV symptoms?
NO
What is HIV seroconversion illness / acute retroviral syndrome and when does it typically manifest?
Seroconversion = body producing antibodies in response to the virus.
SYMPTOMS TYPICALLY OCCUR WITHIN A MONTH OF INFECTION
It is not always accompanied by a flu-like illness, but this is a possibility.
What are the symptoms of acute retroviral syndrome / seroconversion illness?
Fever, lethargy, malaise, fatigue Myalgia, arthralgia Headache Lymphadenopathy Maculopapular rash Pharyngitis Anorexia/nausea/vomiting/weight loss
When primary HIV infection resolves, the body enters a period known as…?
Clinical latency
Outline the diagnostic approach to HIV, including:
- Screening test
- Confirmatory test
- Detection of viral RNA
- Screening test: 4th-generation antibody-antigen test which detects antibodies and HIV antigen (p24 viral core protein) - becomes positive 2-6 weeks after exposure
- Confirmatory test: HIV-1/HIV-2 antibody differentiation immunoassay (first-choice confirmatory test). Can detect both HIV-1 and HIV-2 in ∼20 minutes.
- Detection of viral RNA: detects HIV RNA in serum or plasma and can quantify the number of copies
List 3 advantages of the 4th-generation antibody-antigen test
- High specificity and a sensitivity of almost 100%
- Quicker identification of HIV infection since it looks for the p24 antigen (which appears before antibodies do)
- Can detect HIV as soon as 1 month after infection
Which of the following can be detected the soonest?
- p24 antigen
- RNA
- Antibodies
RNA
Followed by p24 antigen and then antibodies
(look up image of HIV window period)
What is a normal CD4 count?
500-2000
Name 2 opportunistic infections or malignancies that occur at each of the following CD4 counts:
CD4 cell count 200-500
CD4 cell count 50-200
CD4 cell count <50
CD4 cell count 200-500:
Herpes zoster, pneumococcal pneumonia, oral candidiasis, TB
CD4 cell count 50-200:
non-Hodgkin’s lymphoma, kaposi sarcoma (classical tumour associated w/HIV), primary CNS lymphoma, CNS toxoplasmosis, PJP
CD4 cell count <50: disseminated MAC, CMV retinitis, cryptosporidiosis
The WHO classifies individuals with confirmed HIV infection according to clinical features and diagnostic findings. Give an example of a findings that belongs in each of the following categories
- Primary HIV infection
- Clinical stage 1
- Clinical stage 2
- Clinical stage 3
- Clinical stage 4
- Primary HIV infection: acute retroviral syndrome or asymptomatic
- Clinical stage 1: persistent generalised lymphadenopathy (PGL) or asymptomatic
- Clinical stage 2: unexplained moderate weight loss (<10%), recurrent bacterial/viral/fungal infections
- Clinical stage 3: unexplained severe weight loss (>10%), recurrent bacterial/viral/fungal infections, unexplained anaemia, unexplained neutropenia, unexplained persistent fevers, unexplained chronic diarrhoea (>1 month), chronic thrombocytopenia
- Clinical stage 4: AIDS-defining conditions (e.g. kaposi sarcoma, pneumocystitis pneumonia)
A patient presents with chronic thrombocytopenia, unexplained chronic diarrhoea, and unexplained anaemia. 4th-generation antibody-antigen testing reveals that they are HIV-positive.
What clinical stage of HIV infection is this typical of?
Clinical stage 3
A patient presents with kaposi sarcoma. 4th-generation antibody-antigen testing reveals that they are HIV-positive.
What clinical stage of HIV infection is this typical of?
Clinical stage 4
Name 5 risk factors for HIV
MSM (men who have sex with men)
Occupational exposure (e.g. DASA)
IVDU
Coming from a country w/high HIV burden (e.g. south-east asian, africa)
Vertical transmission (mother-to-child)
Why is it not feasible to only test for the p24 antigen?
p24 rises rapidly (~2 weeks in), but also falls as the body mounts an immune response.
This means you have to get tested very early on in the disease, which is difficult because there may be no symptoms
MPPD sexual history osce stuff
.
Is it likely for HIV to be contracted after one sexual encounter?
no
For which patient groups is HIV pre-exposure prophylaxis (PrEP) recommended?
Men who have sex with men
Transgender and gender-diverse people
People who inject drugs
Heterosexual men and women with a HIV-positive partner
(there are guidelines for each group based on high vs. medium risk factors. look @ ETG)
What is involved in PEP?
Take with 72 hours of exposure
28-day course of a 2-drug or 3-drug regimen
Come back for checkups after 1 month, 3 months, and 6 months
What are the indications for HIV post-exposure prophylaxis? (PEP)
3 scenarios
- Injury with HIV-contaminated needles or instruments
- Contamination of open wounds or mucous membranes with HIV-infected fluids
- Unprotected sexual activity with a known or potentially HIV-infected person
When should HIV post-exposure prophylaxis (PEP) be initiated?
How long is it taken for?
As soon as ideally possible (ideally within 2 hours of exposure)
GUIDELINES:
- Within 72 hours of high-risk exposure
- Taken for 28 days
State the route of transmission for Hepatitis A, Hepatitis B, and Hepatitis C
Hepatitis A: Faecal-oral, person-person (e.g. contaminated food or water, household contacts, MSM)
Hepatitis B: Parenteral (e.g. blood, genital secretions), sexual, perinatal
Hepatitis C: Parenteral (intranasal cocaine use is a RF)
State the likelihood of progression to chronic liver disease for Hepatitis A, Hepatitis B, and Hepatitis C
Hepatitis A: Never
Hepatitis B: ~10%
Hepatitis C: ~80%
When it comes to viral hepatitis
When it come to viral hepatitis, vowels (A and E) cause only AcutE hepatitis while Consonants (B, C, and D) may have Chronic sequelae as well.
TRUE OR FALSE? Humans are the only reservoir for Hepatitis A
TRUE
State the following information for HEPATITIS A:
- Incubation period (& average)
- Vaccine available?
- Prevention
- Site of replication
- Symptoms
- Incubation period (& average): 2-6 weeks, with an average of 28 days
- Vaccine available? YES
- Prevention: Vaccine, clean drinking water
- Site of replication: GIT
- Symptoms: fever, jaundice, vomiting, diarrhoea. Usually prodromal symptoms of fever + malaise, THEN jaundice.
Who is more likely to have symptoms of hepatitis A?
Adults
Symptoms are rare in children <5
Can you transmit Hep A infection before you are symptomatic?
What is the infectiousness period?
YES - can be shed in stool 2-3 weeks before symptoms
Infectious 2-3 weeks prior to symptom onset and 1 week after.
TRUE OR FALSE? After contracting Hep A, you develop robust immunity.
TRUE
Which finding on a LFT would indicate Hep A?
Very high ALT (in the thousands)
ALT > AST indicates…
VIRAL HEPATITIS
AST > ALT indicates…
ALCOHOLIC LIVER DISEASE
Which diagnostic tests are used for HAV in the following situations:
- Testing for recent or current infection
- Testing for past infection
- Testing for recent or current infection: IgM antibody
- Testing for past infection/immunity: IgG antibody