Carbon Assimilation Flashcards
Biosynthetic pathway
Sequence of reactions starting with one or more precursor metabolites and leading to synthesis of a building block, cofactor, or secondary metabolite
Carbon assimilation
Converting a carbon source into cell carbon
What are the 7 precursor metabolites of central carbon metabolism?
Glucose 6 phosphate
Ribose 5 phosphate
Phosphoenolpyruvate (PEP)
Pyruvate
Acetyl-CoA
Oxaloacetate
Alpha-ketoglutarate
Precursor metabolites
Intermediates of the central carbon metabolism that are starting points for biosynthetic pathways
Benzoyl-CoA
Key intermediate in anaerobic metabolism
Anaplerotic reactions
“Refill” metabolite pools
How do you go from phosphenolpyruvate directly to oxaloacetate?
Phosphenolpyruvate carboxylase
How do you go from pyruvate directly to malate?
Pyruvate carboxylase
How do you go from malate directly to pyruvate?
Malic enzyme
How do you go directly from phosphenolpyruvate to oxaloacetate?
PEP carboxylase
How do you interconvert between malate and oxaloacetate?
Malate dehydrogenase
How do you convert acetyl-CoA into oxaloacetate? (4 options)
Glyoxylate cycle - 2 acetyl-CoA to oxaloacetate
Ethylmalonyl-CoA pathway - 3 acetyl-CoA + 2 CO2 to 2 oxaloacetate
Methylaspartate cycle - 2 acetyl-CoA to oxaloacetate
Anaerobically
Acetyl-CoA to pyruvate to oxaloacetate
How do you convert glucose-6-phosphate into pyruvate and phosphenolpyruvate?
Phosphofructokinase
Pyruvate kinase gives phosphenolpyruvate
Turn into pyruvate
How do you convert pyruvate or phosphenolpyruvate to glucose-6-phosphate?
Fructose-1,6-bisphosphatase
Then 3 options
Pyruvate and water dikinase = PEP synthase
Pyruvate and phosphate dikinase
Pyruvate carboxylase + PEP carboxykinase
How do you go from acetyl-CoA + oxaloacetate to alpha-ketoglutarate?
Citrate synthase
Aconitase
Isocitrate dehydrogenase
