Carbohydrates + Lipids Flashcards
Chemical formula for alpha glucose
C6H12O6
Maltose structure
Glucose + Glucose
Sucrose structure
Glucose + Fructose
Lactose
Glucose + Galactose
Enzyme suffix
-ase
What 2 subcategories are carbohydrates divided into?
Sugars (mono +di)
Polysaccharides (storage + structural)
Name 2 storage polysaccharides
Starch (plants)
- AmylOse
- Amylopectin
Glycogen (humans)
Name a structural polysaccharide
Cellulose (beta-glucose)
Describe amylOse structure (STARCH)
- Long straight chains of alpha glucose
- COil into a helix (amylOse)
- Compact so good for storage
- Insoluble so doesn’t affect water potential
- Large so cannot diffuse out of cell
Describe amylopectin structure (STARCH)
BRANCHED chain of alpha glucose
- Large surface area for rapid hydrolysis by enzymes (to release glucose for respiration)
- Insoluble so doesn’t affect water potential
- Large so cannot diffuse out of cells
Describe glycogen structure
Similar to starch, however:
- Shorter chains
- More highly branched
- Larger surface area
Explain advantage of glycogen being more highly branched
Glycogen hydrolysed more rapidly into glucose to be used in respiration (as animals require glucose faster than plants can hydrolyse starch)
Describe cellulose structure
- Long straight unbranched chains of beta-glucose
- Every other beta-glucose molecule inverts 180 degrees to form 1-4 glycosidic bond.
- Several chains joined together in layers by MANY WEAK h-bonds to form micro/macrofibrils
What is cellulose found in?
Plant cell wall
Starch use and location
Storage and plant
Glycogen use and location
Storage and animal
Cellulose use and location
Structure and plant
The structure of cellulose is related to its role in plant cell walls. Explain how (3)
- Long straight chains of beta-glucose
- Joined by many weak hydrogen bonds
- To form micro/macrofibrils
- To provide integrity/rigidity
Hydrogen bonds are important in cellulose molecules. Explain why (3)
- Join cellulose chains together to form micro/macrofibrils
- Providing rigidity/integrity to cell wall
- Weak bonds provide strength in larger numbers`
Describe benedicts test for reducing suagr
- Add equal volume of benedicts reagent to solution
- Heat to 95 celcius
- Red precipitate indicates sugar present
Describe how a student would show that a non-reducing sugar was present in a solution (6)
- Complete benedicts test and observe negative result (blue precipitate)
- Add acid (HCL) to solution AND heat to 95 celcius
- Neutralise with alkali
- Add equal volume of benedicts reagent
- Heat again to 95 celcius
- Observe red precipitate formed as reducing sugar is now present
What is an arbitary unit?
A scale of measurement to show the ratio of amount of substance to a predetermined reference measurement
Describe how you would produce a calibration curve for a reducing sugar of unknown concentration and use it to obtain results (5)
- Make up several known concentrations of glucose
- Perform benedicts test on each sample
- Use colorimeter to measure colour intensity of each solution AND plot calibration curve
- Plot known conc. on X-axis and aborbance on Y-axis
- Find concentration of unknown sample using calibration curve
Describe how you would test a food sample for starch
- Add potassium iodide solution to food sample
- Turns purple in presence of starch
Name the 2 types of lipids
- Triglycerides
- Phospholipids
Triglyceride structure
ONE glycerol molecule bonded to 3 fatty acid tails via ester bonds.
Are triglycerides polymers?
No (not made up of repeating units)
Define unsaturated fatty acid
Have double bonds between adjacent carbon atoms within the hydrocarbon chain
Describe the emulsion test for a lipid (liquid)
- Add ethanol
- Mix
- Add water and shake again
- Cloudy white emulsion forms in lipid presence
Describe how you could use the emulsion test to show that a seed contains lipids (3)
- Crush
- Mix with ethanol and shake
- Add water and shake
- Forms cloudy white emulsion in lipid presence
What do phospholipids form in water?
Bilayer
Describe similarities and differences between triglycerides and phospholipids (5)
DIFFERENCE:
- PPL has 2 fatty acid tails, 3rd replaced with hydrophillic phosphate head whereas TRI has 3 fatty acid tails and no phosphate head
- PPL form bilayer in water whereas TRI do not
SIMILARITIES:
- Both contain ester bonds between fatty acid tails and glycerol
- Both contain glycerol and fatty acid tails
- Both fatty acid tails can be saturated or unsaturated