carbohydrates (biological molecules) Flashcards
raffinose is an example of a trisaccharide made up of glucose, fructose, and galactose:
Glucose = C6H12O6
Fructose = C6H12O6
Galactose = C6H12O6
Determine the number of carbon, hydrogen, and oxygen atoms in a single molecule of raffinose (1)
- 18 carbon atoms, 32 hydrogen atoms, 16 oxygen atoms
- two molecules of H2O are lost when forming the glycosidic bonds between the three monosaccharides in a molecule of raffinose
name the type of reaction that would break down raffinose into its monomers
hydrolysis
the monomers of raffinose are known as reducing sugars, describe how you could show that reducing sugars are present in a solution (3)
- add Benedict’s solution
- heat (in a water bath)
- red/orange/yellow/green precipitate will form
give two similarities and two differences between the structure of raffinose and the structure of lactose (4)
- both contain galactose
- both contain glucose
- both contain glycosidic bonds
- raffinose contains fructose, lactose does not
- raffinose is a trisaccharide, whereas lactose is a disaccharide
- raffinose contains 2 glycosidic bonds, whereas lactose contains 1
describe a biochemical test you would carry out to test for the presence of starch in a solution (2)
- add iodine or potassium iodide (to the sample)
- solution turns blue-black (if starch is present)
explain how the structure of starch allows it to function as an energy storage molecule in plants (3)
- insoluble so it doesn’t affect water potential, or does not affect osmosis
- coiled so it is compact
- large molecule so it cannot diffuse out of cells, or cannot cross the cell membrane
- branched so glucose is released quickly, or so it has more ends for fast break down (by enzymes)
describe two similarities between the structure of starch and cellulose (2)
- both are polymers/polysaccharides, or both are made up of monosaccharides
- both contain glucose
- both contain glycosidic bonds, or both contain 1-4 (glycosidic) bonds/links
name the monomer labelled A in the diagram
beta-glucose
name the bond labelled B in the diagram
- glycosidic bond, or 1-4 glycosidic bond
explain how cellulose is adapted for its function in plant cells (3)
- long and straight/unbranched chains
- chains linked together via hydrogen bonds
- form microfibrils
- provide rigidity/strength (to the cell wall)
describe two differences between between the structure of cellulose and starch (2)
differences in overall structure:
- cellulose is straight/unbranched, whereas starch is branched
- cellulose contains microfibrils/macrofibrils, whereas starch does not
differences in structure of monomers:
- cellulose is made up of beta glucose, whereas starch is made up of alpha glucose
- cellulose contains alternating inverted glucose molecules, whereas in starch all glucose molecules are in the same orientation
- cellulose contains only 1-4 glycosidic bonds, whereas starch contains 1-4 and 1-6 glycosidic bonds
name the type of chemical reaction that joins glucose monomers together to form glycogen
condensation
describe how glycogen acts as a source of energy for cells (2)
- hydrolysis releases glucose molecules
- glucose can be used in respiration
describe one structural difference between glycogen and starch (1)
glycogen is more highly branched than starch
explain how glycogens structure enables it to function as an energy storage molecule (4)
- coiled so it is compact
- branched so glucose is released quickly or so more ends for faster hydrolysis
- insoluble so it does not affect water potential, or insoluble, so it is not easily lost from cell
- made up of glucose so glucose can be used in respiration, or made up of glucose, so provides respiratory substrate for energy (release)
name the 2 monomers that make up lactose
- glucose
- galactose
- a student wanted to identify two solutions, A and B, she knew one of the solutions was lactose and the other was galactose, in the first test, she added Benedict’s solution and heated the mixture in a water bath, in the second test, she added the enzyme lactase before adding Benedict’s solution and heating in a water bath, lactase hydrolyses lactose into its monomers
- identify solutions A and B, explain your answer (2)
- A = galactose AND B = lactose
- lactase hydrolysed lactose to give more reducing sugars
describe how the reducing sugars investigation could be changed to give quantitative results, explain why this would improve the experiment (2)
- use a colorimeter
- filter and dry the precipitate, then weigh it
_ - standardises the method
- colour change is subjective
a non-reducing sugar would give a negative result in test 1, describe a third test she could have carried out to identify if a solution is a non-reducing sugar (3)
- heat with acid and then neutralise with an alkali
- or heat with acid and then neutralise with sodium hydrogencarbonate
- heat with Benedict’s solution
- red/orange/green precipitate will form
on the diagram, draw a circle around the chemical groups used to form a glycosidic bond
draw a diagram to show the structure of one sucrose molecule
name the monosaccharides found in a maltose molecule
- glucose
- glucose
a student adds Benedict’s solution to a beaker containing sucrose and heats the mixture in a water bath, no colour change, explain why (2)
- sucrose is a non-reducing sugar
- no acid was used to hydrolyse sucrose into its monomers
what is the chemical formula for glucose
C6H12O6
use the diagram above to draw the structure of an alpha-glucose molecule