Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology VI > Carbohydrates & Amyloid > Flashcards

Carbohydrates & Amyloid Flashcards

1
Q

What control is used for PAS stain?

A

kidney

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the purpose of the PAS reaction?

A

The demonstration of polysaccharides, neutral mucosubstances, and basement membranes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

WhaRedt is another name for the Schiff reagent?

A

leucofuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What reagents on used for the PAS?

A

Periodic acid, HCL, Potassium metabisulfite, Schiff reagent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the oxidizer for the PAS?

A

Periodic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the purpose of the Schiff reagent?

A

Reduction causes the loss of the quinoid structure and masking of the chromphores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What do potassium metabisulfite in the PAS reaction?

A

Remove excess Schiff reagent and prevent false colorization of tissue elements because of oxidation of any adsorbed reagent.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What do you use to check if the Schiff reagent is fresh?

A

Formaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What color will the reaction turn if the Schiff reagent is fresh?

A

reddish purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What color will the reaction turn if the Schiff reagent is bad?

A

deep blue-purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

How must Schiff reagent be stored?

A

In the Fridge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What color is the PAS reaction

A

Bright rose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What control is used for PAS diatase digestion?

A

liver containing glycogen or cervix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the purpose of PAS diastase digestion?

A

Demonstration of glycogen in tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What color will glycogen stain

A

Bright rose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What do the Best carmine stain demonstrate?

A

glycogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Which technique for glycogen is more specific PAS or Best Carmine?

A

PAS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the preferred fixative for PAS?

A

10% NBF or Bouin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Fixative for PAS diatase digestion?

A

10% NBF, formalin alcohol, or absolute alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Fixative for best carmine

A

Absolute alcohol, Carnoy and Bouin can also be used

21
Q

Control for best carmine

A

liver

22
Q

reagents used for best carmine

A

Carmine stock solution, Working carmine solution, differentiating solution

23
Q

What is the counterstain for both PAS reactions?

A

Harris hematoxylin

24
Q

What is Harris hematoxylin used for in the best carmine stain?

A

To stain the nuclei

25
Q

What color is glycogen and Nuclei stained in best carmine?

A

Glycogen-Pink to red

Nuclei- Blue

26
Q

What is the purpose of Mayer mucicarmine?

A

Staining of epithelial mucin in tissue

27
Q

What is the fixative for Mayer mucicarmine?

A

10% NBF

28
Q

Quality control for Mayer mucicarmine

A

unautolyzed colon, small intestine, or appendix

29
Q

Reagents used for Mayer mucicarmine

A

Working mucicarmine solution, Weigert Iron hematoxylin, Mentanil yellow

30
Q

What is the nuclear stain in the Mayer mucicarmine stain?

A

Weigert iron hematoxylin

31
Q

What is the counter stain in the Mayer mucicarmine stain?

A

Mentanil yellow

32
Q

Results of Mayer mucicarmine

A

Mucin-Deep rose to red
Capsule of cryptococcus- Deep rose to red
Nuclei- Black
Other tissue elements- Blue to yellow

33
Q

How can the carminophilic properties be obscured

A

Overstaining with either hematoxylin or metanil yellow

34
Q

What will happen is Gill hematoxylin is used for the nuclear stain of Mayer mucicarmine?

A

The mucin may have a bluish cast

35
Q

Why can you not use autolyzed tissue as a control for Mayer mucicarmine stain?

A

Goblet cells can not be positively identified

36
Q

What is the purpose of Alcian blue, pH 2.5?

A

Demonstration of acid mucopolysaccharides

37
Q

What element do Alcian blue get it color from?

A

copper

38
Q

Fixative for Alcian Blue

A

10% NBF or Bouin

39
Q

Quality control for Alcian blue 2.5 and 1.0

A

unautolyzed small intestine, appendix, or colon

40
Q

Reagent used for Alcian blue 2.5

A

Acetic acid 3%, Alcian blue 1%, Nuclear fast red (kernechtrot)

41
Q

What stain can be used for identification of adenocarcinomas?

A

Mayer mucicarmine

42
Q

What is the counter stain for Alcian blue?

A

Nuclear fast red

43
Q

Results of alcian blue 2.5

A

Weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins- Dark blue
Background-Pink to red
Nuclei- Red

44
Q

Purpose of alcian blue 1.0

A

stain sulfated mucosubstances

45
Q

Reagents of alcian blue 1.0

A

0.1 N HCL, 1% alcian blue, Nuclear fast red

46
Q

Results of alcian blue 1.0

A

sulfated mucosubstances- Pale blue
Background- pink to red
Nuclei- red

47
Q

What is the purpose of Alcian blue-PAS hematoxylin reaction

A

Differentiate between neutral and acidic mucosubstances; used in many laboratories today for the detection of intestinal metaplasia.

48
Q

What is the principle of Alcian blue-PAS-hematoxylin?

A

Acidic mucosubstances are stained with the alcian blue technique and neutral mucosubstances are stained by the PAS reaction

49
Q

Fixative for Alcian blue-PAS-hematoxylin

A

10% NBF or Zenker

Histology VI (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions