Carbohydrates Flashcards
Source of starch
Cassava (Manihot esculenta) / Potato (Solanum tuberosum
Energy storage polysaccharide in plants
Starch
T OR F
Starch is slightly soluble in water
True
Isolation technique of starch
Selective dissolution (addition of H2O)
Visible result in successful isolation of starch
appears as white powder/solid that settled at the bottom of the container.
Polysaccharides can be classified into ____ or ____ depending on the monosaccharide unit that makes up a polysaccharide.
homoglycan or heteroglycan
classification wherein polysaccharide present in the structure is the same all throughout
homoglycan
classification wherein polysaccharides that are made up of two or more saccharide units
heteroglycan
T OR F
Polysaccharide is made up of linear portions all throughout
FALSE
Made up of linear and branched portions
T OR F
Starch is made up of A homoglycan mixture of 3 polysaccharides
FALSE
A homoglycan mixture of 2 polysaccharides:
What are the homoglycan polysaccharides present in the starch
Amylose and Amylopectin
linear homoglycan of glucose connected by α1,4 linkages
Amylose
linkages are flexible allowing the chain to coil (like a telephone cord)
T OR F
I2 will be trapped in the branched structure of amylopectin
FALSE
I2 can be trapped in the coil of amylose to give purple color
general test for the presence of starch
iodine
branched homoglycan w/approx. 1 in 25 glucose linked in both α1-4 & α1-6 fashion
amylopectin
linear part =
branched part =
linear part = α1-4
branched part = α1-6
Glycogen source
Chicken liver (Gallus gallus domesticus)
4 Isolation Technique of glycogen
Homogenization / Lysis
10% TCA (Trichloroacetic acid)
95% EtOH (Ethanol)
NaCl
T OR F
Glycogen is homoglycan
TRUE
Glycogen is made up of
glucose
Glycogen consist of what linkage/s
a 1-4 linkage and a 1-6 linkage
T OR F
Glycogen is branched but it is less branched than the amylopectin of starch
FALSE
Glycogen is more branched than amylopectin of starch
Branch points of
amylopectin of starch:
Glycogen:
amylopectin of starch: every 1 in 25 units
Glycogen: every 1 in 10 units
