Caffiene exctraction

Question 1

Component of tea bag

Answer 1

chlorophyll , caffeine ,cellulose ,tannins

• We won’t find all of them in the tea grain; water soluable will migrate from tea to water ( since they are small molecules
• (Tannin chlorophyll caffeine )<- we drink those
• Cellulose remain in the tea leaves ( since it’s a big molecule it won’t go to the water

Question 2

Purpose of lab

Answer 2

eliminating component to end with caffeine only

To introduce the students into the technique of molecular isolation through extraction
and crystallization.

Question 3

What is the purpose of adding sodium carbonate ( Na2CO3)

Answer 3

sodium carbonate (base) will react with tannins(acid) to neutralize it and the reaction will give us salt of tannins

Question 4

Why shouldnt we have a foam when we shake?

Answer 4

bc when we will add DCM we need 2 layers, if there are foam we I’ll have 3 layers.

(DCM evaporate at warm temp
Liquid liquid extraction: tea+ DCM)

Question 5

What do we have in the dark layer

Answer 5

salt of tannins

Question 6

What do we have in the transparent layer:?

Answer 6

caffeine

Question 7

what will happen to the more soluble in one phase

Answer 7

it will migrate

(Caffein is soluble in water however ,Caffeine is more soluble in DCM which’s why it turned form dark to transparent
/tannins soluble in DCM. but now they are salt which isnt Soluble in DCM)

Question 8

why anhydrous sodium sulfate ?

Answer 8

to remove the dark phase drops ( absorb dark phase)

(Nature of dark phase is aqueous
Sodium sulfate absorb the dark phase since it absorb humidity )

Question 9

what is Anhydrous?

Answer 9

without water

Question 10

Purpose of extracting a component :

Answer 10

• purification
• medicine
• Check for caffeine
• Control check

Question 11

When substance change it should not?

Answer 11

change color

( if color changed that means something else happen. )

Question 12

If you degrade the substance it turn from

Answer 12

brown to black

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