C1- Photosynthesis Flashcards

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1
Q

What are the two stages of photosynthesis?

A

The light dependent stage and light independent stage.

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2
Q

What is the overall equation for photosynthesis?

A

6CO2 + 6H2O → C6H12O6 + 6O2

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3
Q

Define photophosphorylation.

A

An endergonic reaction bonding a phosphate ion to a molecule of ADP using energy from light, making ATP.

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4
Q

Where does the light independent stage occur in a chloroplast?

A

In the stroma- a fluid filled interior bathing the thylakoids and grana.

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5
Q

Where does the light dependent stage occur in a chloroplast?

A

In the grana where the photosynthetic pigments are located.

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6
Q

What are grana?

A
  • Chloroplasts have a double membrane.
  • The inner membranes folds inwards to make thylakoid lamellea.
  • These combine in stacks of up to 100 disc-shaped structures forming grana.
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7
Q

Why will starch grains appear white on electron micrographs?

A

The stain used osmium tetroxide binds to lipids but not carbohydrates.

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8
Q

Where are chloroplasts located in a plant and why?

A

They’re in the stem and leaves- mainly in the palisade mesophyll of the leaves, although they’re also found in the guard cells)

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9
Q

Describe how the structural features of a leaf are significant to photosynthesis.

A

check textbook

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10
Q

Describe how the structural features of a leaf’s cells are significant to photosynthesis.

A

check textbook

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11
Q

Describe how the structural features of chloroplasts are significant to photosynthesis.

A
  • large surface area- maximum light absorption
  • move with palisade cells- move towards the top of the cell on dull days for the maximum absorption of light and on dull days sink to the bottom to protect its pigments from bleaching.
  • rotate with the palisade cells- thylakoids maximise the absorption of light
  • pigments in the thylakoids are in a single layer at the surface of the thylakoid membrane- maximise absorption of light
  • five times as many in the palisade cells as the spongy mesophyll cells- palisade cells are at the top of the leaf so more exposed to light.
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12
Q

Explain what is meant by chloroplasts being transducers.

A

A transducer changes energy from one form into another. Chloroplasts turn energy in photons of light into chemical energy, made available through ATP and incorporated into molecules such as glucose.

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13
Q

What is a pigment?

A

A molecule that absorbs specific wavelengths of light.

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14
Q

Why are there different pigments within chlorophyl?

A

Allows for a large range of wavelengths to be absorbed and is consequently more useful than there was just one pigment present absorbing a small range of wavelengths.

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15
Q

What are the two main classes of pigment in flowering plants?

A
  • chlorophylls

- carotenoids

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16
Q

What are the two pigments in the chlorophylls class and what are their colours?

A
  • chlorophyll a- yellow-green

- chlorophyll b- blue-green

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17
Q

What are the two pigments in the carotenoids class and what are their colours?

A

B-carotene- orange

xanthophyll- yellow

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18
Q

What is an absorption spectrum?

A

A graph showing how much light is absorbed at different wavelengths.

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19
Q

What is an action spectrum?

A

A graph showing the rate of photosynthesis at different wavelengths.

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20
Q

What does a correlation between the action spectrum and absorption spectrum suggest?

A

The pigments are responsible for absorbing the light used in photosynthesis.

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21
Q

Where are photosystems located?

A

In the plane of the thylakoid membrane.

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22
Q

What two things is a photosystem comprised of?

A

An antenna complex and a reaction centre.

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23
Q

What is an antenna complex?

A

An array of protein and pigment molecules in the thylakoid membranes of the grana that transfer energy from light of a range of wavelengths to a chlorophyll a, at the reaction centre.

24
Q

Describe the features of an antenna complex containing photosynthetic pigments in a photosystem.

A
  • Chlorophylls and carotenoids are anchored into the phospholipids of the thylakoid membrane.
  • They’re held together by protein molecules in clusters of up to 400 molecules.
  • Each cluster is called an antenna complex.
  • The combination of pigments allows light at a range of wavelengths to be absorbed.
25
Q

Describe the features of a reaction centre in a photosystem.

A
  • located within an antenna complex
  • contains two molecules of the primary pigment chlorophyll a.
  • when the chlorophyll a molecules absorb light, their excitation allows each one to emit an electron.
  • there are two types of reaction centres- PSI and PSII
26
Q

What are the two types of reaction centres?

A

Photosystem I (PSI):

  • arranged around a chlorophyll a molecule
  • absorption peak of 700nm
  • also called P700

Photosystem II (PSII):

  • discovered after PSI
  • arranged around a chlorophyll a molecule
  • absorption peak of 680nm
  • also called P680
27
Q

How are photons absorbed?

A
  • some are absorbed by chlorophyll a directly, but many are absorbed by chlorophyll b and the carotenoids which, are the accessory (or antenna) pigments.
  • the photons excite the accessory pigments and energy is passed through them to the reaction centre where electrons of chlorophyll a are excited and raised to a higher energy level.
28
Q

Why is chlorophyll a referred to as the primary or core pigment?

A

It is the most significant molecule of the reaction centre because it passes energy to the subsequent reactions of photosynthesis.

29
Q

What reaction uses light as an energy source and water?

A

The light dependent stage reactions

30
Q

What does the light dependent stage produce?

A
  • ATP- which provides chemical energy transducer from light energy
  • reduced NADP- which provides the reducing power to synthesise molecules such as glucose from carbon dioxide
  • oxygen- a by-product derived from water- it diffuses out of the leaf
31
Q

What light independent reactions occur and why are they known as this?

A
  • reactions using ATP and reduced NADP make molecules such as glucose occur in solution in the stroma
  • they can happen in light but do not require it so are described as light independent
  • they include a cycle of reactions called the Calvin cycle
32
Q

What is cyclic photophosphorylation?

A

ATP can be produced by electrons that take a cyclic pathway and are recycled back into the chlorophyll a in PSI.

33
Q

What is non-cyclic photophosphorylation?

A

ATP can be produced by electrons that take a linear pathway from water through PSII and PSI to NADP, which they then reduce.

34
Q

Describe cyclic photophosphorylation in detail.

A
  • PSI absorbs photons that excite electrons in the chlorophyll a molecules in its reaction centre.
  • The electrons are emitted and picked up by an electron acceptor, which passes them down a chain of electron carriers back to the PSI.
  • The energy released as they’re passed down the electron transport chain phosphorylates ADP to ATP.
35
Q

Describe non-cyclic photophosphorylation in detail.

A
  • electrons from PSI are transferred from the electron acceptors to oxidise NADP in the stroma, which protons from the photolysis of water reduce
  • the electrons are not returned to PSI so the chlorophyll is left with a positive charge.
  • the positive charge is neutralised by electrons from PSII which have been excited to a higher energy level by the absorption of light, picked up by an electron acceptor and passed down the electron transport chain to the PSI.
  • the electrons passage down the electron transport chain makes energy available for the phosphorylation of ADP to ATP.
  • the chlorophyll in PSII is left with a positive charge and is neutralised by the electrons released in the photolysis of water.
36
Q

Define photolysis.

A

The splitting of water molecules by light producing hydrogen ions, electrons and oxygen.

37
Q

Where does photolysis occur and why?

A

In the thylakoid spaces water molecules absorb light which indirectly causes them to dissociate into hydrogen, oxygen and electrons.

38
Q

Which stage of photosynthesis does non-cyclic photophosphorylation and cyclic photophosphorylation occur in?

A

the light dependent stage of photosynthesis

39
Q

What is the equation for the photolysis of water?

A

H2O → 2H^+ + 2e^- + 1/2O2

40
Q

What is photolysis enhanced by?

A

A protein complex in PSII, which is the only known enzyme that causes water to be oxidised.

41
Q

In photolysis where do each of the products go?

A
  • The electrons go to PSII to replace those lost to PSI
  • The protons from water and the electrons from PSI reduce NADP
  • Oxygen diffuses out of the chloroplast and cell, out through the stroma as a waste product.
42
Q

How is the oxygen plants released produced?

A

By the photolysis of water.

43
Q

Describe the passage of protons in the light dependent stage of photosynthesis.

A
  • electrons pass through the proton pump in the thylakoid membrane providing energy to pump protons from the stroma into the thylakoid space.
  • the protons join H^+ ions from the photolysis of water accumulating so that an electrochemical gradient is created- a source of potential energy
  • chemiosmosis then occurs- H^+ ions diffuse down their electrochemical gradient, through ATP synthetase in the thylakoid membrane into the stroma
  • this makes the energy from derived from light available as when they pass through ADP is phosphorylated to ATP.
  • Once in the stroma H^+ ions are passed to oxidised NADP reducing it.
  • The removal of H^+ ions in conjugation with the proton pump, contributes to maintaining the proton gradient across the thylakoid membrane.
44
Q

What is the Z-scheme?

A

The pathway taken by electrons in non-cyclic photophosphorylation.

45
Q

What is the equation for the reduction of NADP?

A

NADP + 2H^+ + e^- → reduced NADP

46
Q

What three factors maintain the proton gradient between the thylakoid space and stroma?

A
  • the proton pump associated with the electron transport chain pushing protons into the thylakoid space
  • the photolysis of water in the thylakoid space
  • the removal of protons from the stroma reducing NADP
47
Q

What is the overall equation for the light-dependent stage of photosynthesis?

A

NADP + H2O + 2ADP + 2Pi → reduced NADP + 1/2O2 + 2H2O + 2ATP

the 2H2O and 2ATP come from the condensation of 2ADP + 2Pi

48
Q

What are the similarities between cyclic and non-cyclic photophosphorylation?

A
  • PSI

- ATP production

49
Q

What are the differences between cyclic and non-cyclic photophosphorylation?

A
  • non-cyclic photophosphorylation uses PSII, but cyclic photophosphorylation doesn’t
  • the chlorophyll at the reaction centre in non-cyclic photophosphorylation is P680, but P700 for cyclic photophosphorylation
  • non-cyclic photophosphorylation is linear, but cyclic photophosphorylation is cyclic
  • non-cyclic photophosphorylation produces oxygen
  • non-cyclic photophosphorylation involves the reduction of NADP
  • non-cyclic photophosphorylation involves the photolysis of water
  • cyclic photophosphorylation is in all photosynthetic organisms, non-cyclic photophosphorylation is in plants, algae and cyanobacteria
50
Q

How does the light independent stage of photosynthesis relate to the light dependent stage?

A

It uses the products of the light dependent stage:

  • ATP is an energy source
  • reduced NADP is the source of the reducing power- reducing carbon dioxide
51
Q

Describe the Calvin cycle.

A
  • ribulose bisphosphate (5C) (RuBP) combines with carbon dioxide, catalysed by the enzyme rubisco
  • an unstable six carbon compound is formed
  • this immediately splits into two molecules of glycerate-3-phosphate (3C) (GP)
  • GP is reduced to triode phosphate by reduced NADP- this requires energy which is provided by ATP made in the light dependent stage
  • triose phosphate is the first carbohydrate made in photosynthesis
  • NADP is reformed
  • some triose phosphate is converted to glucose phosphate and then into starch by condensation
  • most triose phosphate goes through a series of reactions that regenerates RUBP so that the cycle can continue- ATP from the light dependent stage provides energy for this to occur.
52
Q

What enzyme catalyses the reaction of RuBP with carbon dioxide?

A

ribulose bisphosphate carboxylase- AKA rubisco

53
Q

Explain how the Calvin cycle was discovered.

A

pg 30 second year textbook

54
Q

How are carbohydrates produced within plants.

A
  • they’re produced from the triose phosphate
  • the first hexose made is fructose phosphate, which can be converted to glucose and combined with glucose to make sucrose for transport around a plant
  • the a-glucose molecules may be converted into starch for storage or B-glucose which is polymerised into cellulose for cell walls.
55
Q

How are fats produced within plants.

A
  • acetyl coenzyme A (AcCoA) can be synthesised from glycerate-3-phosphate in the calvin cycle and converted into fatty acids
  • triose phosphate can be converted directly into glycerol.
  • Fatty acids and glycerol undergo condensation reactions to form triglycerides
56
Q

How are proteins produced within plants.

A
  • glycerate-3-phosphate from the Calvin cycle can be converted into amino acids for protein synthesis
  • the amino acid group is derived from NH4^+ ions made from nitrate ions taken in at the roots and transported throughout the plant