[C] Chromatography Flashcards
What is chromatography used for?
To separate mixtures into their individual components.
True or False: Chromatography can only be used for colored substances.
False
What are the two main phases in chromatography?
The stationary phase and the mobile phase.
Fill in the blank: In paper chromatography, the stationary phase is _____ paper.
filter
What is the purpose of the solvent in chromatography?
To carry the substances through the stationary phase.
Which factor affects the distance a substance travels in chromatography?
The affinity of the substance for the stationary phase versus the mobile phase.
Multiple Choice: What is the stationary phase in thin-layer chromatography?
A solid adsorbent material.
What does Rf value stand for?
Retention factor.
How is Rf value calculated?
Distance traveled by the substance divided by distance traveled by the solvent.
True or False: A higher Rf value indicates a greater affinity for the stationary phase.
False
What is the role of a pencil in paper chromatography?
To mark the baseline without interfering with the separation.
Fill in the blank: The _____ phase is usually a liquid in liquid chromatography.
mobile
What type of substances can be separated using gas chromatography?
Volatile substances.
Multiple Choice: In gas chromatography, what is the mobile phase?
A gas.
What is the significance of the separation in chromatography?
It allows for the identification and analysis of individual components.
True or False: Chromatography can be used for both qualitative and quantitative analysis.
True
What is the purpose of spotting in chromatography?
To apply a small amount of the mixture onto the stationary phase.
Fill in the blank: In chromatography, substances with a _____ affinity for the mobile phase travel further.
higher
What can affect the results of chromatography?
Temperature, solvent choice, and the type of stationary phase.
Multiple Choice: Which of the following is NOT a type of chromatography?
Magnetic chromatography.
What is the first step in a chromatography experiment?
Prepare the stationary phase.
True or False: Chromatography can be performed in a closed system.
True
What should be recorded during a chromatography experiment?
The distance traveled by the solvent and each substance.
Fill in the blank: The _____ of the solvent front should be marked after the experiment.
position
What is one application of chromatography in real life?
Testing for drugs in urine.
Multiple Choice: Which type of chromatography is best for separating complex mixtures?
High-performance liquid chromatography (HPLC).
What does the term ‘baseline’ refer to in chromatography?
The starting line where the mixture is applied.
True or False: The results of chromatography can be visualized using UV light.
True
What is one disadvantage of paper chromatography?
Limited resolution for closely related compounds.
Fill in the blank: Chromatography is often used in _____ analysis.
chemical
What is the main advantage of gas chromatography?
High sensitivity and resolution for volatile compounds.
