C albicans Culture Techniques Flashcards
1
Q
What is the use of a solid medium (agar plates)?
A
Single colony isolation
Colony count
2
Q
What is the use of a liquid medium?
A
Growth assay - incubate flask then obtain aliquots (e.g., for optical density reading)
3
Q
What is the purpose of reconstituting a specific gene back into the strain?
A
To make sure that any loss in function is due to the gene
4
Q
How many mice need to be infected by each strain?
A
At least 5
5
Q
What does the Kaplan-Meier plot show?
A
Percentage survival of mice over time
6
Q
How can ammonia released by C albican strains be quantified?
A
Placed colonies over citric acid trap, ammonia quantified using Nessler’s reagent.
7
Q
What is the use of measuring LDH released from cells?
A
It is a marker of cell lysis
