Buffers

Question 1

MD037

Answer 1

Feed Media. WFI, Soytone, Yeast Extract, Soy 100, Potassium Phosphate Mono/Dibasic, Dextrose, Ammonium chloride. Soy 100 has high amounts of nucleosides, free amino acids, vitamins, and carbohydrates. Dextrose provides more sugars

Question 2

MD038

Answer 2

Growth Media. WFI, Soytone, Yeast Extract, Potassium Phosphate Mono/Dibasic, Glycerin

Question 3

SL060

Answer 3

17% Phosphoric Acid. Fermentation

Question 4

SL001

Answer 4

Lysis Resuspension Buffer. 50 mM Dextrose, 25 mM Tris, 10 mM EDTA. Tris-EDTA solubilizes nucleic acids and helps protect them from enzymatic lysis. EDTA also binds divalent metals and destabilizes the cell membrane. Dextrose maintains cellular osmolarity to prevent them from bursting

Question 5

SL002

Answer 5

0.2N NaOH, 1% SDS. SDS solubilizes the phospholipid and protein components of the cell membrane. NaOH denatures plasmid DNA and proteins

Question 6

SL048

Answer 6

0.4 M NaOH, SL002 component

Question 7

SL047

Answer 7

2% SDS, component of SL002

Question 8

SL003

Answer 8

Alkaline Lysis Neutralization Buffer. 3M potassium acetate, 6.75% acetic acid. Causes KDS (potassium dodecyl sulfate), ssDNA, and cell debris to precipitate while allowing p-DNA to renanneal

Question 9

SL042

Answer 9

Alkaline lysis precipitation buffer. 3.84M CaCl. Helps precipitate RNA

Question 10

SL017

Answer 10

Used during DEAE equilibration, DEAE wash 2, DEAE elution. ~1.12 M NaCl, 50mM Tris, 10mM EDTA, HCl

Question 11

SL018

Answer 11

Used during DEAE/HLD equilibration, DEAE wash 1/2, HLD wash 1/2/elution, HLD Resin Strip

Question 12

SL021

Answer 12

Used for HLD equilibration, loading (diluent), HLD Wash 1/2/elution. 3M ammonium sulfate 50mM Tris, 10mM EDTA

Question 13

SL019/40

Answer 13

DEAE Resin Strip. 1M NaOH, 2M NaCl. SL040 uses liquids, SL019 uses solids

Question 14

SL020

Answer 14

20% EtOH, DEAE monolith storage

Question 15

0.1 M NaOH

Answer 15

HLD monolith storage

Question 16

Lysis Buffers

Answer 16

1. SL001
2. SL002 = SL047 + SL048
3. SL003
4. SL042

Question 17

1 M NaOH

Answer 17

Chromatography Wash buffer

Question 18

SL067

Answer 18

Final Formulation Buffer. 10mM Tris, 1mM EDTA, HCL