BSL SHIT Flashcards
Used to study moderate-risk agents that pose a danger if accidentally inhaled, swallowed, or exposed to the skin. Safety measures include limited access, biohazard warning signs, sharp precautions, class I or II BSCs, the use of PPE such as gloves and eyewear, as well as hand washing sinks and waste decontamination facilities such as an autoclave.
BSL-2
Includes organisms like Escherichia coli O157:H7, Hepatitis A virus, and Neisseria meningitidis, which require containment practices to prevent accidental exposure
BSL-2
Laboratories classified under this biosafety level handle agents with moderate risk and require additional precautions like face shields, autoclaving waste, and limited personnel access.
BSL-2
Agents associated with human disease and pose moderate hazards to personnel and the environment. Examples include Human adenoviruses, human herpes viruses (except herpes B), Staphylococcus aureus, Salmonella typhimurium, and human tissue, blood, and cell lines.
BSL-2
Requires specific safety measures such as restricted laboratory access, biosafety cabinets, PPE usage, and decontamination procedures to handle pathogens with moderate risk of infection.
BSL-2
A workstation designed solely to protect the work provides no protection for the employee or the surrounding room, allowing airflow to move across the work surface and into the employee’s face. It is commonly used for tasks such as tissue culture and pouring gels, with no specific testing requirements. Proper disinfection before and after each use is essential.
LAMINAR FLOW HOOD
Also referred to as clean air devices (CADs)
★ These devices only provide product
protection
★ They can be used for certain clean
activities, such as the dust-free assembly
of sterile equipment or electronic devices
★ Clean benches should never be used
when handling cell culture materials, drug
formulations, potentially infectious
THE HORIZONTAL/VERTICAL LAMINAR
FLOW CLEAN BENCH
(not BSCs)
focuses on protecting personnel, the community, and the environment from the unintentional release of hazardous materials and preventing the theft, loss, and misuse of hazardous biological agents, toxins, equipment, or valuable information for malicious use. A successful containment strategy must incorporate aspects of both biosafety and laboratory security to adequately address the risks present at the facility.
LABORATORY BIOSECURITY
★ Enclosures that capture and contain
biohazardous aerosols within the cabinet
★ Major component include:
○ High Efciency Particulate Air
(HEPA) lter
■ Ultra-Low Particulate Air
(ULPA) lters - for special
applications
○ Motor/Blower
○ Appropriate air intakes, du
BIOLOGICAL SAFETY CABINETS
● Remove most penetrating particle size
(MPPS) of ~0.3 um with 99.99% efciency
● Made of single sheet of borosilicate bers
treated with a wet-strength,
water-repellant binder
● More effective for both larger and smaller
particles (bacterial spores, viruses)
HEPA FILTERS
is designed for cell culture and handling small quantities of toxic volatile chemicals in in vitro biological systems. Like Type A1 and A2 cabinets, it has a split downflowing air stream above the work surface. Air moving toward the rear grille is discharged into the exhaust system, preventing chemical vapor buildup. To ensure safety, activities involving volatile chemicals should be performed near the rear of the cabinet
CLASS 2, TYPE B1 BSC
A total-exhaust biosafety cabinet does not recirculate air, ensuring complete removal of contaminants. Germ-Free invented and patented this design. It offers primary containment for both biological agents and small quantities of chemicals, making it ideal for handling hazardous materials safely.
CLASS 2, TYPE B2 BSC
Examples of microorganisms handled in this biosafety level include Mycobacterium tuberculosis, Venezuelan Equine Encephalitis, and Francisella tularensis.
BSL-3
Requires high-efficiency particulate air (HEPA) filters before air leaves the building, and filters must be changed every 6 months or annuall
BSL-3
Must use Class I or II biosafety cabinets, and safety features include unidirectional airflow, HEPA-filtered air, and controlled access to prevent exposure to airborne pathogens.
BSL-3
Used to study agents that can be transmitted through the air and may cause potentially lethal infections. Safety features include clothing decontamination, sealed windows, double-door access, and specialized ventilation systems
BSL-3
Requires the use of biosafety cabinets for all procedures involving viable agents and the use of solid front gowns, scrubs, and two pairs of gloves.
BSL-3
Used to study agents that pose a high risk of life-threatening disease, aerosol-transmitted lab infections, or related agents whose risk is not known.
BSL-4
Lab personnel working in this biosafety level must shower when exiting the facility to prevent contamination.
BSL-4
This biosafety level incorporates all BSL-3 features but is located in a safe, isolated zone within a larger building or in a separate facility.
BSL-4
Procedures in this biosafety level must be performed in Class III biosafety cabinets or in Class II cabinets while wearing a positive-pressure full-body suit.
BSL-4
Examples of agents handled at this biosafety level include Ebola virus, Marburg virus, Lassa virus, and Herpes B virus.
BSL-4
The laboratory director is primarily responsible for assessing risks and appropriately applying recommended biosafety levels in this high-containment lab.
BSL-4
Special practices in this biosafety level include mandatory clothing changes before entry, daily inspections of containment systems, decontamination of all wastes before removal, and mandatory showering upon exit.
BSL-4
degrades over time losing its
effectiveness as a disinfectant
degrades at a rate of 20%
per year
○ A 1:10 has a shelf
life of 24 hours when kept in an
open container.
bottles should be dated when
received and used within one year
○ Or use at higher concentrations to
account for 20% degradation per
year
○ Be mindful that chlorine is rapidly
lost with exposure to organic
materials
It Rhymes With Bitch
BleacH BAnkai
Pathogenic microorganisms that are
present in human blood or other
potentially infectious materials (OPIM)
and can cause disease
(BBP) include, but
are not limited to:
○ HIV
○ Hepatitis B
○ Hepatitis C
○ Malaria
○ Dengue
BLOODBORNE PATHOGENS
Use of a physical or chemical procedure to
destroy all microbial life, including high
resistant bacterial endospores
STERILIZATION
Kills virtually all non-spore-forming
microorganisms but not necessarily all
microbial forms on inanimate objects
(work surfaces, equipment, etc.)
★ Effectiveness is inuenced by the kinds
and numbers of organisms, the amount of
organic matter, the object to be
disinfected and chemical exposure time,
temperature and concentration
○ Pseudomonas spp. - sensitive to
high-level disinfectants
DISINFECTION
Process or treatment that renders a
laboratory device, instrument, or surface
safe to touch
★ Can range from sterilization to simple
cleaning with soap and water
★ Forms of decontamination:
○ Sterilization
○ Disinfection
○ Antiseps
DECONTAMINATION
Bleach OR autoclave liquid biowaste
before sink disposal.
★ Use 10% bleach, mix well, let sit 20 mins,
dispose
★ Do not autoclave any chemicals including
bleach.
★ Do not mix bleach with other
disinfectants or chemicals, bleach is an
oxidizer and can react violently with other
chemicals
DECONTAMINATION - LIQUID BIOWASTE
DISPOSAL
Designed for work with highly infectious
microbiological agents and the conduct of
hazardous operations
★ Provides maximum protection for the
environment and the worker
★ A gas-tight enclosure with a non- opening
view window.
CLASS 3 BSC
Variable,Sterilization/Variable Intermediate to high-level disinfection
Glutaraldehyde
0.55% - High-level disinfection clue oppa gangnaman style
Ortho-phthalaldehyde (OPA)
6–30% - Sterilization/3–6% - Intermediate to high-level disinfection
Hydrogen peroxide
6–8% - Sterilization/1–8% - Low- to high-level disinfection
Formaldehyde
Variable - Sterilization/Variable - High-level disinfection
Chlorine dioxide
0.08%–0.23% with peroxide concentrations of 1–7.35% - Sterilization/Variable - High-level disinfection
Peracetic Acid
500–6000 mg/L Free available - Intermediate to high-level disinfection
Hypochlorites
70% - Intermediate-level disinfection
Alcohols (ethyl, isopropyl)
0.5–3% - Low- to intermediate-level disinfection
Phenolics
30–50 mg/L Free - Low- to intermediate-level disinfection
Iodophors
Variable - Low-level disinfection
Quaternary Ammonium Compounds
