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Microbiology Finals > Brucella > Flashcards

Brucella Flashcards

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1
Q

Haemophilus influenze
Classification

Based on capsular antigen

A

Serotype

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2
Q

Haemophilus influenze
Classification

Based on chemical properties

A

Biotype

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3
Q

Haemophilus influenze
Classification

Useful for clinical purposes

A

Biogroup

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4
Q

Haemophilus influenze
Culture

Flat grayish brown colonies after 24 hours incubation

A

Chocolate agar

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5
Q

Haemophilus influenze
Culture

Does not grow on

A

Sheep blood agar

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6
Q

Haemophilus influenze

Requires

A 
X factor (Hemin)
V factor (NAD)
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7
Q

Table of characteristics of growth and requirements

A

Memorize

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8
Q

Haemophilus influenze
Virulence factor

Anti phagocytic
Main virulence factor
Type b

A

Capsule

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9
Q

Haemophilus influenze
Virulence factor

Lipooligosaccharide saccharides (endotoxin)

A

Somatic antigen

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10
Q

Haemophilus influenze
Type B

Most common serotype causing systemic disease

A 
Meningitis
Pneumonia and emphysema
Epiglottitis
Cellulitis
Septic arthritis
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11
Q 
Haemophilus influenze
Non typable (non encapsulated)
A 
Sinusitis
Opportunistic
Chronic bronchitis
Conjuctivitis
Otitis media
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12
Q

Haemophilus influenze

Secondary to bacteremic spread from nasopharynx

A

Meningitis

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13
Q

Haemophilus influenze

Reddish blue patches on cheeks or peri orbital areas

A

Cellulitis

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14
Q

Haemophilus influenze

Children

A

Arthritis

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15
Q

Haemophilus influenze

Epidemic or endemic
H. Influenza bio group aegypticus

A

Conjunctivitis

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16
Q

Haemophilus influenze

Prevention

A

Chemoprophylaxis with Rifampicin

Hib conjugate vaccine

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17
Q

Haemophilus aegypticus

A

Knock weeks bacillus

Resembles H. Influenza closely

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18
Q

Haemophilus aegypticus

Diseases

A

Conjuctivitis

Brazilian purpuric fever

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19
Q

Chancroid
Ulcer on genitalia
Painful and enlarged lymph nodes

A

Hemophilus ducreyi

Best on chocolate agar

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20
Q

Bordatella pertusis

A 
Gram negative
Coco bacillary 
Encapsulated
Non motile
Strict aerobes
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21
Q

Bordatella pertusis

With bipolar metachromatic granules

A

Toluidine blue stain

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22
Q

Bordatella pertusis

Form acid from

A

Glucose

Lactose

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23
Q

Bordatella pertusis

Culture

A

Bordet gengou medium
Potato blood glycerol agar
Contain Pen G.

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24
Q

Bordatella pertusis

Virulence genes

A

bvgA and

bvgA

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25
Q

Bordatella pertusis
Virulence factors

Protein on pili

A

Filamentous hemagglutinin

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26
Q

Bordatella pertusis
Virulence factors

Lymphocyte do not enter lymphoid tissue
Sensitization to histamine
Enhanced insulin secretion

A

Pertusis toxin

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27
Q

Bordatella pertusis
Virulence factors

Inhibit phagocytosis, increase cAMP

A

Adenyl cyclase toxin

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28
Q

Bordatella pertusis

Adheres to and multiplies rapidly on epithelial surface of

A

Trachea

Bronchi

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29
Q

Bordatella pertusis

MOT

A

Airborne droplets

30
Q

Bordatella pertusis

Source of infection

A

Catarrhal stage

31
Q

Bordatella pertusis

Disease

A

Pertusis or whooping cough

32
Q

Bordatella pertusis

Incubation pwriod

A

2 weeks

33
Q

Bordatella pertusis

Stages

A

Catarrhal- highly infectious but not very ill
Paroxysmal - hacking coughs, “whoops”
Convalescence- slow

34
Q

Bordatella pertusis
Diagnosis

Specimen

A

Saline nasal wash (preferred)

Nasopharyngeal swab

35
Q

Bordatella pertusis
Diagnosis

50% sensitivity

A

Direct fluorescence antibody test

36
Q

Bordatella pertusis
Diagnosis

Most sensitive

A

PCR

37
Q

Bordatella pertusis
Diagnosis

On 3 rd week of illness

A

Serology

38
Q

Bordatella pertusis

Chemoprophylaxis

A

Erythromycin

39
Q

Bordatella pertusis

Vaccines

A

Acellular vaccine - inactivated pertusis vaccine

DPT x 3 doses

40
Q

Brucella

A 
Zoonotic
Gram negative
Coccobacilliary
Aerobic
Non motile
Non spore forming
41
Q

Brucella

Positive in

A

Catalase
Oxidase
Urease

42
Q

Brucella

Culture

A

Soy agar

Blood culture media

43
Q

Brucella
Virulence factor

Anti phagocytosis

A

Fusion of lysosomal granules

44
Q

Brucella
Virulence factor

Sugar found in animal placenta

A

Tropism for erythritol

45
Q

Brucella

MOT

A

Ingestion
Droplets
Skin contact
Inhalation

46
Q

Brucella

Species of brucellosis

A

Melitensis- goats - acute and sever infection
Suis - swine - suppurative lesions, caseating granuloma
Abortus - cattle - no suppurative lesions, non caseating granuloma
Canis - dogs - mild disease

47
Q

Brucella

Clinical brucellosis ( undulant or Malta fever)

A

Acute- influenza like syndrome

Chronic - non bacteremic, psychoneurotic

48
Q

Brucella
Diagnosis

Serology

A

Increase IgM during 1st week of illness, peak at 3 months

49
Q

Brucella

Treatment

A

Tetracycline+streptomycin

Trimethoprim - sulfamethazole

50
Q

Pasteurella

A

Non motile

Aerobes or facultative aerobes

51
Q

Pasteurella

Postive

A

Oxidase

Catalase

52
Q

Pasteurella

Most common organism in human wounds inflicted by bites from cats and dogs

A

Pasteurella multocida

53
Q

Pasteurella multocida

Treatment

A

Pen G doc

Tetracyclines and Fluoroquinolones alternatives

54
Q

Francisella Tularensis

Culture

A

Small colonies within 1-3 days in
Cysteine blood agar or
Glucose blood agar

55
Q

Francisella Tularensis

Mot

A

Skin abrasion (most common)

Bite of arthropod
Direct contact with infected animal tissue
Inhalation of aerosol
Ingestion of contaminated food or water

56
Q

Francisella Tularensis

Tularemia
Incubation period

A

3-5 days

57
Q

Francisella Tularensis

Tularemia
Sign and symptoms

A

Ulceroglandular- ulcers at site of infection

Oculoglandular- direct eye inoculation

58
Q

Francisella Tularensis

Tularemia

Treatment

A

Live attenuated vaccine

Streptomycin

59
Q

Yersinia

A

Bipolar staining
Gram negative
Microaerophillic or facultative anerobic

60
Q

Yersinia

Positive

A

Positive catalase

Negative oxidase

61
Q

Yersinia
Virulence factor

Anti phagocytic

A

Fraction 1

62
Q

Yersinia
Virulence factor

Essential for virulence

A

72- kb plasmid

63
Q

Yersinia

MOT

A

Bite of rat flea

Inhalation of infective droplets

64
Q

Yersinia pestis

Clinical manifestations

A

Bubonic plague- inflamed enlarged LN

Pneumonic - sepsis, death

65
Q

Yersinia pestis

Diagnosis

A

Smears- giemsa, fluorescent, wayson’s stain
Culture- blood agar, mocConkey’s
Serology

66
Q

Yersinia pestis

Treatment

A

Streptomycin doc

Tetracycline alternative

67
Q

Y. Enterocolitica

A

Contaminated food
Fomites
Abdominal pain, diarrhea
Mimic appendicitis

68
Q

Yersinia pseudo tuberculosis

A

Fecal oral route

Caseous nodules in the payers patches and mesenteric nodules

69
Q

Yersinia enterocolitica and pseudo tuberculosis

Treatment

A

3rd generation

Cephalosporin and aminoglycosides
Fluoroquinolones and another anti microbial

Resistant to ampicillin and 1stgen

70
Q

Heamophillus

A

Grams negative
Facultative anaerobes
Obligate parasites

Microbiology Finals (1 decks)

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