BPE Flashcards

(118 cards)

1
Q

What are the 4 examples of cell host?

A
  1. Bacteria
  2. Yeast
  3. Mammalian cells
  4. Insect
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2
Q

What is biopharmaceutical engineering?

A

The application of engineering principles to enhance the performance and production of biopharmaceutical products.

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3
Q

What is a signal peptide

A

A short peptide(16-30 amino acids long) present at the N-terminus

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4
Q

What is the N-terminus

A

The start of a protein or peptide

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5
Q

What is the first step in the process?

A

Objective

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6
Q

What does HEK cell stand for?

A

Human embryonic kidney cell.

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7
Q

What is the first step of the design process?

A

Objective

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8
Q

What is the second step of the design process?

A

Design Criteria

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9
Q

What is the third step of the design process

A

Measure

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10
Q

What is the fourth step of the design process?

A

Test

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11
Q

What is the fifth step of the design process?

A

Model

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12
Q

What is the sixth step of the design process

A

Design

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13
Q

What is the seventh step in the design process?

A

Validate

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14
Q

What is the eighth step in the design process?

A

Benefits

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15
Q

What are the 3 structures in the central dogma of molecular biology?

A

DNA, RNA, protein

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16
Q

What process converts DNA to RNA?

A

Transcription

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17
Q

What process converts RNA to DNA?

A

Reverse transcription

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18
Q

What process converts RNA to protein?

A

Translation

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19
Q

What are the products when cells are used as factories?

A

RNA, Proteins

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20
Q

What are the products when the cell is used as the product?

A

Cell therapies

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21
Q

What are the 3 parts of DNA?

A

Promoter - Coding sequence Ancillary DNA

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22
Q

What do promoters do?

A

Dictate the kinetics of gene expression

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23
Q

What are motifs?

A

Building blocks of DNA that bind proteins, interact with receptors and form secondary structures

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24
Q

How can DNA be redesigned?

A

Combining motifs in optimal order

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25
What are the objectives for DNA in manufacturing?
Optimising expression levels, minimising silencing and instability, and minimising off target effects.
26
What are the objectives when using DNA as products?
Increase specificity/ reduce off-target, optimise gene expression, Minimise immunogenicity, Max half-life.
27
What is the structure of mRNA?
5'UTR - Coding Sequence - 3'UTR (Poly A Tail)
28
Which parts of the mRNA control the level of expression?
ALL 3!
29
What controls the 'other functionalities in DNA and mRNA?
Generic DNA the coding sequence.
30
How is mRNA redesigned?
Using multi-parametric optimisation, might do 500 base pairs in one go
31
What is a sliding window?
A tool used in mRNA engineering that can help optimise 50 base pairs at a time, as opposed to working with the whole sequence
32
What is mRNA engineering all about?
Avoiding making the mRNA 'bad'
33
What are the goals when using mRNA for manufacturing?
Maximising expression levels and minimising off target effects on the cell factory
34
What are the goals when using mRNA as products?
Maximising gene expression level in target cell, Minimise immunogenicity and maximise half-life.
35
What is the structure of a protein?
Signal Peptide ---> Protein sequence
36
What do signal peptides control?
Protein transport/expression rate
37
What controls the functional effect of a protein?
Protein sequence
38
What is a protein?
A string of amino acids
39
What dictates how a protein works?
The secondary structures produced from amino acid folding.
40
What are the building blocks in protein engineering?
Motifs that interact with proteins, secondary structures that interact with proteins/receptors/DNA/mRNA & perform functions.
41
What part of the protein is used when it is being used for manufacturing?
Signal peptide
42
What are the goals when using proteins for manufacturing?
Optimising expression levels, minimising off target effects.
43
What are the goals when using signal peptides as products?
Maximise gene expression level in cell.
44
What are the goals when using proteins as products?
Maximise activity / effectiveness,, minimise off target effects, encode cell type specificity
45
What are the type of products relevant to this course?
DNA, mRNA, proteins, cells
46
What is the goal in the objective step?
What are you trying to achieve, value statement. All tech should be driven to achieve a measurable target.
47
What is the goal in the design criteria step?
What features will the engineered biological component/technology need to have?
48
What are the 2 types of measuring technique?
Top-down, bottom-up
49
Explain top-down measuring, simply
Want to understand the whole system
50
Explain bottom-up measuring, simply
Want to understand individual building blocks
51
What question are you answering in the measure step?
What do you need to quantify?
52
What are OMICS?
Methods of measurements including genomics, proteomics, metabolomics, metagenomics and transcriptomics.
53
What is transriptomics?
A measurement techniques that analyses which genes are expressed and to what level
54
What is proteomics?
Measurement technique that analyses what proteins are in the cell
55
What questions are you answering in the test phase?
How do you test hypotheses about how the system works?, How to obtain data that expands knowledge of system working
56
What is the aim of the model step?
Building a model that facilitates design of new biological components.
57
What are you answering when modelling
How much data do you need? Can you minimise the number of test components?
58
What is the question in the design step?
How can you convert a model into designed components? How big does the library need to be to provide a high chance of success while maintaining realistic timings and costs?
59
Explain the Validate step
Are you using industry relevant testing? How many components to take forward? How to move up TRL scale
60
explain benefits
How are you going to describe/sell the benefits of your tech
61
What are the main 3 benefits of biopharma engineering?
Increasing speed to market Reduced cost per dose Better patient outcomes
62
How is mRNA product made?
in vitro transcription, using RNase T7 as a catalyst
63
What is the issue with mRNA input components?
Expensive, unreliable supply chains
64
mRNA is a simple system, what is the problem with this?
May not be able to produce complex products at high quality.
65
What are some things to do when designing mRNA production platform?
Improved Quality High Transcription minimise cell specific productivity High cell productivity High cell growth Cheap/reliable input materials.
66
What host cell will be used for mRNA manufactue and why?
Bacteria, because mRNA is easy to make and bacteria is a simple host.
67
What should be a key consideration when designing mRNA
It has a short half life in E. Coli, so key design criteria could be enhancing mRNA stability in E. Coli
68
What can we engineer when a cell is producing mRNA?
How much mRNA the cell makes, how quickly it degrades.
69
What could increase stability in mRNA?
A more complex structure such as a circle,as then the enzyme can't digest/degrade it.
70
What makes a good control system?
- Representative of industry - Not purposefully 'hobbled' - Well understood and recognised
71
What are the 2 types of test?
DoE and OFAT
72
what does DoE stand for?
design of experiments
73
what does OFAT stand for
One factor at a time
74
What does TRL stand for?
Technology Readiness Level
75
What TRL step do most projects fail on?
4/5
76
What is TRL 1
Basic principles observed and reported
77
What is TRL 4
Lab testing of component or process
78
What is TRL 5
Lab testing of integrate system
79
What is TRL 6
Prototype system verified
80
What is TRL 8
System complete and qualified
81
What is TRL 9
System proven in operational environment.
82
What can be done to validate past TRL5
Scale-up, compare to existing biology Optimisation of processing, Quality analytics
83
What are some validation steps?
Manufacture product, Use the product in reactions, Compare to incumbent tech
84
What is a DNA plasmid?
A small circular, double stranded DNA molecule, distinct from the cells chromosomal DNA
85
What are some benefits of using DNA plasmids?
They are cheap and simple to manufacture, compared to AAV) and relatively stable compared to mRNA, there is also no restrictions on cargo size
86
What are the drawbacks of using a DNA plasmid?
It is inherently immunogenic
87
What are dendritic cells
A type of cell found in tissues that boosts immune response by showing antigens on its surface to other cells in the immune system
88
What is the function of TNF alpha?
Ut is responsible for inflammation and a range of signalling events
89
What are some issues in DNA plasmid design?
Immunostimulation causing off target effects Receptors that recognise foreign DNA is poorly understood. DNA plasmids are lost quickly from cells, reducing expression per dose.
90
How can you pick biomarkers?
Use bottom up and top down measurements to study markers and see what elicits a response out of the target cell.
91
What is important to show for a new biotech
Genes identified are biomarkers Tool doesn't produce false negs/positives Tool accurately identifies small differences immunostimulatory behaviour.
92
What could you do to validate a technology?
Get companies to test designs in the new tech and then in patients.
93
What are some design criteria when engineering DNA gene therapies?
Reduce activation of immune response Whilst keeping gene expression and manufacturability the same
94
What is a homotypic library
A data set created by trying the design criteria one at a time
95
What is important about heterotypic libraries?
They need to be systematic to test design hypotheses
96
How big should the library be?
Large enough to decipher required rules but small enough to fit cost & time objectives.
97
What happens if a DNA gene therapy exists in a patient's genomic DNA?
Cancer
98
What are some design criteria for a DNA gene therapy
Boosted early expression/retention Increased plasmid retention rate Boosted Late expression
99
What does recombinant mean?
relating to or denoting an organism, cell, or genetic material formed by recombination
100
What cell is used for most recombinant protein production?
CHO cell factories
101
How are proteins made using CHO cells?
DNA is inserted into the cell genome that encodes for the expression of a new product. Followed by selection of a 'top' clone which is used in large-scale bioreactor to produce kg quantities.
102
What are limitations of CHO cells
Most CHO cell alternatives are good at making 'vanilla' products but struggle with next-gen products.
103
What are the key issues with CHO cell factories?
Low productivity, cell death, poor product quality and aggregation
104
What are the limitations of in vitro protein production
Hard to scale, cant handle next-gen, high costs
105
Benefits of in vitro protein production?
It would be more standardised and predictable. also more engineerable.
106
What is qP
Cell specific recombinant protein production rate
107
Design criteria for CHO cell line
Increase qP, maintain high cell growth rate, Improve product quality. Extras: INcreased average clone perfomance
108
What is measured in CHO cells?
Genes, introducing and deleting
109
What OMICs are done in CHO cell line development
Almost always transcriptomics
110
What is a phenotype
The characteristics that the cell exhibits
111
What is a signal peptide?
A short amino acid sequence that controls the rate of translocation(movement of a protein through a cell)
112
What percentage of protein's have a signal peptide?
about 35% - All vaccines, cell therapies and recombinant proteins
113
What is the DNA expression construct?
Promoter - 5'UTR - signal peptide - coding sequence - 3'UTR
114
What is the mRNA expression construct?
5'UTR - signal peptide - coding sequence - 3' UTR
115
Why are people reluctant to manufacture signal peptides?
As opposed to DNA, by messing up you can change the product you are making.
116
What are the 5 problems with signal peptides?
1. Performance is product specific 2. No-one knows how signal peptides work 3. Signal peptides are hard to test 4. Because they are part of the product there is problems with regulations 5. It is hard to future proof your solutions.
117
What are the historical challenges with signal peptide manufacture?
Highly product-specific Poorly understood High 'failure-rate' Off -target effects
118
How are DNA gene therapies switched on?
By entering the cell.