Bordtella

Question 1

Medium used for bordtella and what is cough plate method?

Answer 1

Bordet-Gengou glycerine potato blood agar
Cough plate method: hold agar plate 15-30cm within pt mouth so when he has a bout of cough his respiratory droplets fall on the medium directly.

Question 2

B. pertussis stages in pathogenicity

Answer 2

After 1-2 weeks incubation Period
3 stages:
① catarrhal stage: low grade fever, dry coughing, max infectivity, clinical diagnosis difficult
② paroxysmal stage: experience paroxysms ( violent spasms of continuous coughing followed by long rush of air into empty lungs, with a whooping sound)
③ convalescence stage: gradual decrease in coughing.

Question 3

Prophylaxis for pertussis

Answer 3

① whole killed vaccine: immunisation with killed B. pertussis
Inactivation should kill but be antigenic. Detoxification with 0.2% merthiolate.
Alum absorbed vaccine more protection. Triple vaccine with diphtheria & tetanus toxoid. B pertussis also an adjuvant for these toxoids.
3 injections before 6 months age at 4-6 weeks intervals.
② acellular vaccine: contains protective components of bacillus(PT etc)