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Question 1

How does detection take place, what dyes

Answer 1

EtbR
SYBR
RedSafe
Prop Iodine

Question 2

What are the six requirements for ideal primer design

Answer 2

• 18-24BP
• 40-60 GC
• ends with 1/2 GC pairs
• melt temp 50-60 with 5 between
  not complementstry

Question 3

When is taq thermostable

Answer 3

70-80 but up to 97
lower error rate than poly 1

Question 4

5 applications of PCR

Answer 4

Gene Mapping
Quantification
Site Directed Mutagenisis
Selective Amplification
DNA sequencing

Question 5

What is the last step of RT PCR

Answer 5

• PCR on sample w/ transgene from specific primer

Question 6

What must U3 promoters have

Answer 6

TATA box
monocot specific promoter
upstream sequencer element

Question 7

Methods to screen tarsngene

Answer 7

T7E1
deep sequencing
sanger sequencing
restriction digestion

Question 8

Transcription is controlled by

Answer 8

enchancer + silencer
TATA box
reg DNA
trascr factors

Question 9

6 methods of gene profiling

Answer 9

microarray
single cell
northern blotting
RT PCR
cDNA
Bulk RNA

Question 10

tools for RNA seq

Answer 10

453
Illumini
third gen SMRT

Question 11

Limitations of RNA seq

Answer 11

ligation bias
high abundance transcr
5% give rise to 75%

Question 12

methods for generating haploid

Answer 12

microspore
anther culture
interspecific crossing
naturally occuring haploid