Body Fluids Final Flashcards
volume of the total ruled area of one side of the hemocytometer
0.9uL
RBC square volume (one side)
0.02uL
Test order for tubes of CSF
Tube 1: Chem and serology
Tube 2: Micro
Tube 3: Hematology
Tube 4: Misc
Which CSF tubes must be centrifuged prior to testing?
Chem and micro
What is the diagnostic descriptor for yellow discoloration of CSF supernatant?
Xanthrochromic
Signs of traumatic tap (2)
Decreasing blood in tubes
Bloody, clotted tube
Signs of CVA in CSF (3)
Xanthrochromia
RBC degradation products
All tubes bloody
adult normal range for CSF chemistry (3)
Protein: 15-45mg/dl
Glucose: 45-80mg/dl OR
60-70% of plasma glucose
adult normal ranges for CSF hematology (2)
WBC: <5 cells/uL
RBC: 0 cells/uL
normal adult diff for CSF? (4)
Lymph: 70%
Mono: 30%
Neutro: 0
May also report as 100% mono
Normal volume of CSF in children
10-60ml
Normal volume of CSF in adults
90-200ml
How are childrens’ normal results for CSF different than adults? (2)
Newborns can have 30 monos
“flipped diff,” more monos than lymphs
Failure to document time a semen is collected primarily affects interpretation of what?
viscosity/liqueficatoin
sperm motility evaluations should be performed within ___ hours
1-2
sperm motility is evaluated on the basis of (2)
speed and direction
The major component of seminal fluid is
fructose
proteolytic enzymes may be added to semen specimens to
decrease the viscosity
The immunobead test for antisperm antibodies determines the presence of ___ (3)
IgG, IgM, and IgA antibodies
Presence of prostatic acid phosphatase and glycoprotein p30 can determine possible ____ (2)
sexual assault
Monosodium urate crystals in synovial fluid are
gout
Calcium pyrophosphate crystals in synovial fluid are
pseudogout
Cholesterol is present in synovial fluid when there is
chronic inflammation/extracellular
Corticosteroid is present in synovial fluid after
steroid injections
Calcium oxalate can be found in synovial fluid during
renal dialysis
Apatite is found in synovial fluid of those with
osteoarthritis
The highest WBC in synovial fluid is expected with (2)
septic arthritis
To disperse cells in synovial fluid for counting/slide prep, ______ can be added to decrease viscosity
hyaluronidase
monosodium urate crystal shape
needles
Calcium pyrophosphate crystal shape
rhombic rods
cholesterol crystal shape
notched rhombic plates
corticosteroid crystal shape
flat, variable shaped plates
calcium oxalate crystal shape
envelopes
apatite crystal shape
small particles
In pleural fluid, a decrease of _________ is indicative of TB
mesothelial cells
which tests are used to differentiate transudate and exudates 100% of the time? (3)
Specific gravity
Lactate dehydrogenase
Total protein
removal of ascetic fluid from the abdominal area
paracentesis
normal peritoneal volume
100ml
normal pericardial volume
50ml
normal pleural volume
30ml
Where could you find chylous or pseudochylous material?
Thoracic cavity
Pericardial cavity
Abdominal cavity
excess fluid as a result of systemic disruption
transudate
excess fluid as a result of localized damage
exudate
pH of 6.0 in pleural fluid usually indicates
esophageal rupture
RBC count of >100,000 in a peritoneal lavage indicates
blunt force trauma to abdominal area
why do most fluids require concentration before differential? (2)
ensure max cells available
decrease time required
what is the main purpose of performing a diff? (2)
identify nucleated cell types
differentiate nucleated cell types
what is the stain of choice for a diff?
Wright’s or Wright-Geimsa
which fluid type is most likely to have tumor cells? lease likely?
most: serous
least: synovial
how are cells categorized on a diff? (3)
Neutrophils
mononuclear
misc/abn cells
what is the easiest way to differentiate amniotic fluid and maternal urine?
creatinine
amniotic fluid is collected in amber containers to prevent destruction of
bilirubin
purpose of Liley graph
monitor severity of HDFN vs. fetal age of survivability
when performing an L/S ratio by TLC, a mature lung will show
lecithin twice as concentrated as sphingomyelin
Tests to determine FLM
Phosphatidyl glycerol
Amniostat
foam stability
lamellar bodies
L/S ratio by TLC
what do you look for in a vaginal wet prep
trichomonas
yeast
clue cells
Clue cell vaginal conditions
pH<4.5
positive amine
positive whiff test