Body Fluids Final Flashcards

1
Q

volume of the total ruled area of one side of the hemocytometer

A

0.9uL

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2
Q

RBC square volume (one side)

A

0.02uL

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3
Q

Test order for tubes of CSF

A

Tube 1: Chem and serology
Tube 2: Micro
Tube 3: Hematology
Tube 4: Misc

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4
Q

Which CSF tubes must be centrifuged prior to testing?

A

Chem and micro

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5
Q

What is the diagnostic descriptor for yellow discoloration of CSF supernatant?

A

Xanthrochromic

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6
Q

Signs of traumatic tap (2)

A

Decreasing blood in tubes
Bloody, clotted tube

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7
Q

Signs of CVA in CSF (3)

A

Xanthrochromia
RBC degradation products
All tubes bloody

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8
Q

adult normal range for CSF chemistry (3)

A

Protein: 15-45mg/dl
Glucose: 45-80mg/dl OR
60-70% of plasma glucose

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9
Q

adult normal ranges for CSF hematology (2)

A

WBC: <5 cells/uL
RBC: 0 cells/uL

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10
Q

normal adult diff for CSF? (4)

A

Lymph: 70%
Mono: 30%
Neutro: 0
May also report as 100% mono

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11
Q

Normal volume of CSF in children

A

10-60ml

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12
Q

Normal volume of CSF in adults

A

90-200ml

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13
Q

How are childrens’ normal results for CSF different than adults? (2)

A

Newborns can have 30 monos
“flipped diff,” more monos than lymphs

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14
Q

Failure to document time a semen is collected primarily affects interpretation of what?

A

viscosity/liqueficatoin

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15
Q

sperm motility evaluations should be performed within ___ hours

A

1-2

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16
Q

sperm motility is evaluated on the basis of (2)

A

speed and direction

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17
Q

The major component of seminal fluid is

A

fructose

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18
Q

proteolytic enzymes may be added to semen specimens to

A

decrease the viscosity

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19
Q

The immunobead test for antisperm antibodies determines the presence of ___ (3)

A

IgG, IgM, and IgA antibodies

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20
Q

Presence of prostatic acid phosphatase and glycoprotein p30 can determine possible ____ (2)

A

sexual assault

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21
Q

Monosodium urate crystals in synovial fluid are

A

gout

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22
Q

Calcium pyrophosphate crystals in synovial fluid are

A

pseudogout

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23
Q

Cholesterol is present in synovial fluid when there is

A

chronic inflammation/extracellular

24
Q

Corticosteroid is present in synovial fluid after

A

steroid injections

25
Q

Calcium oxalate can be found in synovial fluid during

A

renal dialysis

26
Q

Apatite is found in synovial fluid of those with

A

osteoarthritis

27
Q

The highest WBC in synovial fluid is expected with (2)

A

septic arthritis

28
Q

To disperse cells in synovial fluid for counting/slide prep, ______ can be added to decrease viscosity

A

hyaluronidase

29
Q

monosodium urate crystal shape

A

needles

30
Q

Calcium pyrophosphate crystal shape

A

rhombic rods

31
Q

cholesterol crystal shape

A

notched rhombic plates

32
Q

corticosteroid crystal shape

A

flat, variable shaped plates

33
Q

calcium oxalate crystal shape

A

envelopes

34
Q

apatite crystal shape

A

small particles

35
Q

In pleural fluid, a decrease of _________ is indicative of TB

A

mesothelial cells

36
Q

which tests are used to differentiate transudate and exudates 100% of the time? (3)

A

Specific gravity
Lactate dehydrogenase
Total protein

37
Q

removal of ascetic fluid from the abdominal area

A

paracentesis

38
Q

normal peritoneal volume

A

100ml

39
Q

normal pericardial volume

A

50ml

40
Q

normal pleural volume

A

30ml

41
Q

Where could you find chylous or pseudochylous material?

A

Thoracic cavity
Pericardial cavity
Abdominal cavity

42
Q

excess fluid as a result of systemic disruption

A

transudate

43
Q

excess fluid as a result of localized damage

A

exudate

44
Q

pH of 6.0 in pleural fluid usually indicates

A

esophageal rupture

45
Q

RBC count of >100,000 in a peritoneal lavage indicates

A

blunt force trauma to abdominal area

46
Q

why do most fluids require concentration before differential? (2)

A

ensure max cells available
decrease time required

47
Q

what is the main purpose of performing a diff? (2)

A

identify nucleated cell types
differentiate nucleated cell types

48
Q

what is the stain of choice for a diff?

A

Wright’s or Wright-Geimsa

49
Q

which fluid type is most likely to have tumor cells? lease likely?

A

most: serous
least: synovial

50
Q

how are cells categorized on a diff? (3)

A

Neutrophils
mononuclear
misc/abn cells

51
Q

what is the easiest way to differentiate amniotic fluid and maternal urine?

A

creatinine

52
Q

amniotic fluid is collected in amber containers to prevent destruction of

A

bilirubin

53
Q

purpose of Liley graph

A

monitor severity of HDFN vs. fetal age of survivability

54
Q

when performing an L/S ratio by TLC, a mature lung will show

A

lecithin twice as concentrated as sphingomyelin

55
Q

Tests to determine FLM

A

Phosphatidyl glycerol
Amniostat
foam stability
lamellar bodies
L/S ratio by TLC

56
Q

what do you look for in a vaginal wet prep

A

trichomonas
yeast
clue cells

57
Q

Clue cell vaginal conditions

A

pH<4.5
positive amine
positive whiff test