Blotting Flashcards
Who discovered southern blotting?
Edwin M Southern
What does southern blotting detect?
DNA
Probe used in the southern blotting
DNA or RNA
Probe used in northern blotting
DNA or RNA
Probe used in western blotting
Antibody
Probes used in eastern blot
Protein
What is the pH of the buffer in Southern blotting?
pH 7 or 9
What is added in the buffer of southern blot and why?
Urea to prevent the DNA from getting coiled
Who discovered northern blotting?
Alwin and Stark
How to prevent mRNA from getting coiled?
Adding formaldehyde or urea and increasing the pH
Who discovered western blotting and who gave the term western blotting?
Neal Burnette gave the term
Towben, Renart and Reiser discovered it
What is added in the membrane to prevent antibodies getting binded to free space?
Non specific protein
Far western blot used for?
To detect proteins but using non antibody probe
South Western blot used for?
To detect DNA binding protein
Eastern blot
To analyse protein post translational modification. Mostly to detect carbohydrates epitopes
Lectin is used to determine? Annexin V used to determine?
Lectin - carbohydrates
Annexin V - phosphoryl serine
Far eastern used for?
Analysis of lipids separated by high performance tlc
Dot blotting
To detect proteins and nucleic acid
Not in gel but directly on membrane
