Blood Specimen Collection and Handling Flashcards

1
Q

Method for BGA

A

Arterial Puncture

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1
Q

Commonly used method for CBC

A

Venipuncture

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2
Q

Ideal for single withdrawal of blood sample

A

Syringe Method

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3
Q

Ideal for multiple blood sampling

A

Evacuated Tube System Method (ETS)

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4
Q

Site for venipunture

A

Antecubital fossa

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5
Q

Ideal location for venipuncture for M-pattern

A

1st: median vein of the forearm
2nd: median cephalic/median basilic
3rd: cephalic/basilic

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6
Q

Ideal location for venipuncture for H-pattern

A

Median Cubital Vein

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7
Q

What site is not allowed for obese patients?

A

Wrist

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8
Q

Used when there’s no available veins in the arms and forearms of the patient.

A

Veins in the lower extremities

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9
Q

Not allowed for lower extremities venipuncture

A
  • compromised patients
  • patients with sickle cell anemia
  • elderly patients
  • atherosclerosis plaque patients
  • diabetic patients
  • patients with Hemoglobin S.
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10
Q

Also called sickle cell anemia

A

HgSS

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11
Q

Also called sickle cell trait

A

HgAS

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12
Q

Sickle cell diseases

A
  • HgSD
  • HgSG
  • S-Beta-Thalassemia
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13
Q

Trapped in capillaries and blood vessels which will then form clusters resulting in decrease blood flow

A

Sickle-shaped cells

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14
Q

Does not need fasting and diet restriction

A

Hemoglobin Test

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15
Q

Needs fasting 6-8 hours to ensure that there will be no additional turbidity in the plasma

A

Special Hematology Test - Platelet Aggregation Studies

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16
Q

Patients must not take aspirin for at least how many days?

A

7 days

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17
Q

Patients must not take aspirin for at least 7 days or NSAIDs

A

Special Hematology Test - Platelet Function

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18
Q

Have anti-platelet activity

A

NSAIDs

19
Q

Needs fasting for fasting blood sugar

A

Clinical Chemistry

20
Q

CBC Measures _?

A
  • Hemoglobin (Hgb)
  • RBC Count
  • WBC Count
  • Platelet Count
  • Hematocrit (HcT)
  • Differential Count
  • Mean Corpuscular Volume (MCV)
21
Q

Calculated parameters which is affected by the values of hemoglobin

A

Red cell indices (MCH, MCHC, MCV)

22
Q

Colorimetric method for Hgb determination

A

Cyanmethemoglobin

23
Q

Turbid plasma results to what?

A

False increase of Hgb levels

24
Q

Happens when patient’s blood after meal affects the turbidity of the plasma

A

Chyle formation

25
Q

In cyanmethemoglobin, how many hours is needed before blood collection?

A

2 hours

26
Q

Drugs affecting false elevation of WBC count

A
  • Epinephrine
  • Cortisol administration
27
Q

Drugs affecting elevation of neutrophil

A
  • lithium-containing drugs
  • corticosteroids
  • cortisol
  • digitalis
  • phenylhistine drugs
28
Q

Drugs affecting reduction of neutrophil

A
  • amidopyrine drugs
  • aminopyridine drugs
29
Q

7-8% higher concentration and blood count in this position

A

Sitting

30
Q

Diluted blood in this position

A

Lying down

31
Q

Used for hematology section

A

EDTA (anticoagulated tube); CBC

32
Q

Used for PT and PTT

A

Coagulated blood; Sodium Citrate

33
Q

PT meaning

A

Prothrombin Time

34
Q

PTT meaning

A

Partial Thromboplastin Time

35
Q

Used for EOFT Studies

A

Heparinized tube

36
Q

Used for Clinical Chemistry

A

fasting blood sugar; fluoride tube (gray)

enzyme test; red top for serum

37
Q

Angle of insertion of needle

A

15-30 degrees

38
Q

Distance of tourniquet from puncture site

A

3-4 inches (10-15 cm)

39
Q

Done to remove cellular debris

A

Scrubbing

40
Q

In antiseptic solution, how much percent is not used because it evaporates quickly?

A

90-100%

41
Q

Parameters affected by povidone iodine

A
  • Potassium
  • Phosphate
  • Urates
42
Q

Test for alcohol determination

A

Benzalkonium chloride

43
Q

Results to decreased blood count

A

Collapsed Vein

44
Q
A