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Hema Lab > Blood Smear Preparation > Flashcards

Blood Smear Preparation Flashcards

1
Q

POIKILOCYTOSIS:
Thin rim of hbg at the periphery and a large area of central pallor

A

Leptocyte

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2
Q

POIKILOCYTOSIS:
Cigar-shaped RBC

A

Elliptocyte

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3
Q

What happens if smear is examined for more than 5 hours?

A

Result to unacceptable blood artifacts

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4
Q

How many days do RBCs remain in the circulation?

A

120 days

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5
Q

High quality smears can be obtained if they are done ________of drawing blood.

a. 1-2 hours
b. 2-3 hours
c. 3-4 hours

A

b.

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6
Q

Sickle or crecent shaped RBCs

A

Leptocyte

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7
Q

Normal Erythroid Series:
1. No nuclei are present
2. Produced pink and blue color cytoplasm thus resulting in a murky gray blue
3. First stage in which pink color is associated with hgb can be seen
4. Last stage capable of mitosis

A

Polychromatic Normoblast

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8
Q

The polychromatic erythrocytes move out in to the peripheral blood for about how many day/s before reaching maturity?

A

1 day

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9
Q

What results from an angle that is increased?

A

Thick smear

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10
Q

Sickle or crecent shaped RBCs

A

Leptocyte

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11
Q

Anticoagulant of choice for blood smear preparation.

A

EDTA

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12
Q

The wedge smear (manual wedge technique) requires 2 glass slides having how many inches or mm?

A

3 inch by 1 (75mm x 25mm)

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13
Q

Increased number of red cells with variation in shape

A

Poikilocytosis

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14
Q

What results from an angle that is decreased?

A

Thin smear

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15
Q

For a well-made wedge peripheral film, the film should be _____ to _____ the length of the slide.

A

2/3 to 3/4 respectively

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16
Q

The most convenient method and widely used technique for smearing.

A

Wedge smear (manual wedge technique)

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17
Q

POIKILOCYTOSIS:
Fragmented RBCs

A

Schistocyte

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18
Q

High quality smears can be obtained if they are done ________from drawing blood.

a. 1-2 hours
b. 2-3 hours
c. 3-4 hours

A

b.

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19
Q

What results from an increased pressure/speed?

A

Thin smear

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20
Q

POIKILOCYTOSIS:
Oval shaped RBCs

A

Ovalocyte

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21
Q

What results from an increased size of blood?

A

Thick smear

22
Q

Normal Erythroid Series:
1. No more mitosis
2. Nucleus is pyknotic
3. Increase in salmon pink color of cytoplasm
4. Late in stage, nucleus is extruded

A

Orthochromic Normoblast (Metarubricyte/Late Normoblast)

23
Q

Normal Erythroid Series:
1. No more mitosis
2. Nucleus is pyknotic
3. Increase in salmon pink color of cytoplasm
4. Late in stage, nucleus is extruded

A

Orthochromic Normoblast (Metarubricyte/Late Normoblast)

24
Q

POIKILOCYTOSIS:
Elongated RBCs with a slit-like central pallor

A

Stomatocyte

25
Q

This refers to the older technique, is inconvenient, and only advantage is excellent leukocyte distribution.

A

Cover glass smear (coverslip technique)

26
Q

POIKILOCYTOSIS:
RBCs with regularly spiculated surface

A

Echinocytes (Burr Cells)

27
Q

POIKILOCYTOSIS:
Pear-shaped or teardrop shaped RBCs

A

Dacryocyte

28
Q

What results form a decreased pressure/speed?

A

Thick smear

29
Q

How many days do the polychromatic erythrocytes resides in the bone marrow?

A

1 DAY

30
Q

Normal Erythroid Series:
1. Contains one or two nuclei
2. Round or slightly oval
3. Central or slightly eccentric nucleus
4. Cytoplasm is dark blue
5. Gives rise to two daughter pronormoblast
6. Globin production begins

A

Pronormoblast (Rubriblast)

31
Q

POIKILOCYTOSIS:
1. Almost spherical in shape
2. Lacks central pallor

A

Spherocyte

32
Q

Normal Erythroid Series:
1. Chromatin begins to condense
2. Nucleoli present early and disappear later
3. Cytoplasm is deeper rich blue

A

Basophilic Normoblast (Prorubricyte/Early Normoblast)

33
Q

Blowing breath on a slide is not recommended. Why is this so?

A

Moisture in breath can cause RBCs to become echinocytic or develop water/drying artifact.

34
Q

Increased number of red cells with variation in size.

A

Anisocytosis

35
Q

Variation in normal coloration that may also mean the occurrence of hypochromic and normochromic cells in the blood smear.

A

Anisochromia

36
Q

Normal Erythroid Series:
1. No nucleus
2. Last stage of hemoglobin production

A

Polychromatophilic (Reticulocyte)

37
Q

What results from a decreased size of blood?

A

Thin smear

38
Q

Increased number of red cells with variation in size.

A

Anisocytosis

39
Q

Why should slides be dried as quickly as possible?

A

To avoid artifact formation

40
Q

This requires an automated slide making and staining system.

A

spun smear

41
Q

For wedge smear, the capillary tube should be filled about ______- of blood.

A

3/4

42
Q

What are the features of a well-made wedge peripheral film?

A
  1. Film is 2/3 to 3/4 the length of the slide
  2. Film is finger-shaped
  3. Visible lateral edges
  4. Film is smooth without irregularities, holes, and streaks
  5. Feather edge has a rainbow appearance (if held against the light)
  6. The whole drop of blood is picked up and spread.
43
Q

Permits effective assessment of RBC production by the bone marrow.

A

Reticulocyte Count

44
Q

Measure of effective erythropoiesis.

A

Reticulocyte

45
Q

Associated with impaired DNA synthesis

A

Macrocytes

46
Q

Associated with defected hemoglobin formation

A

Microcytosis

47
Q

Four ways to detect RBC anomalies

A
  1. Nucleus of a small lymphocyte
  2. MCV Values
  3. Using RDW
  4. Histogram
48
Q

POIKILOCYTES:
Semi-circular indent on th edge of their membrane

A

Bite cells

49
Q

The largest cell in the bone marrow

A

Mature megakaryocytes

50
Q

Part of a platelet that is granular and located centrally

A

Chromomere

51
Q

Part of a platelet that surrounds the chromomeres, non-granular, and clear to light blue

A

Hyalomere

Hema Lab (1 decks)

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