Blood component & Inventory Flashcards
1
Q
Blood products
A
RBC, PLT, Granulocytes, Plasma (cryoprecipitate, albumin, IVIG), Hematopoietic SC, Coagulant factors
2
Q
Apheresis products
A
- Double unit RBC
- Apheresis PLT: HLA, HPA, Multi-dose
- Jumbo plasma
- Plasma products
- Granulocytes
- Stem cells
3
Q
Whole Blood composition
A
- Vol: 450-500mL
- Content: RBC, Plasma, WBC (10^9) and PLT, anticoagulant added to the bag
- Still used in cases: ABO identical, Autologous, Trauma/massive transfusion, Places w/o processing
4
Q
Packed RBC (PRBC)
A
- WB: soft spin –> separate from PLT rich plasma
- Apheresis: directly
- 1 unit RBC increase Hgb 1g/dL or HCT 3%
5
Q
PRBC properties
A
- V = 300mL
- RBC (200mL) + Plasma, anticoagulant, additive solution, WBC, PLT
6
Q
Anticoagulant & Additives
A
- Anticoagulant: CPD (citrate phosphate dextrose), CPD2 (2x dextrose), CPDA-1 (+adenine) –> 21 days shelf-life
- Additives: AS-1 (Adsol) CPD + mannitol, AS-3 (nutricell), AS-5 (Optisol): CPD & mannitol –> 42 days shelf-life
7
Q
Storage temp for PRBC
A
1-6 C for RBC in storage
1-10 C for RBC in transport
-65C for frozen RBC in storage (deep freeze)
8
Q
PLT component
A
- From WB: random/pooled. Pool 4-6 units to have enough for an adult therapeutic dose. 1 unit = 50mL
- From apheresis PLT: 1 unit is 1 therapeutic dose. Decrease donor exposure and bacterial contamination
9
Q
FFP
A
- V = 250mL
- Cell free component of blood: water, electrolytes, lipids, carbohydrate, proteins
- FFP (FP8), FP24, TP: INR 0.9 - 1.5, reduced factor 5, 8
10
Q
Plasma properties
A
- Dose: therapeutic 10-20 mLs/kg –> 2 units = standard dose (300x2 = 600mL)
- Transient: due to factor 7
- Effect is more at higher INR (high risk of bleeding)
11
Q
FFP preparation
A
- V = 200-250mL
- Content: all coagulant factors, no viable cells, anticoagulant
- Storage: Freeze within 8 hrs or 6 hrs. Store up 1 year at -18C or 7 year at -65C
- Effect: 10-15mL/kg dose raise level 20-30% in 70kg adult. Transient. Plasma Ab must be compatible.
12
Q
FP24 preparation
A
- V = 200-250mL
- Produced when WB can’t be prepared within 8 hours
- Content: F5 & protein S: slightly less. F13: 25% less
- Storage: freeze within 24 hrs. Store up 1 year at -18C. Don’t use to make Cryo-
13
Q
Thawed Plasma
A
- FP24 and FP8 thawed at 37C, store at 1-6C
- Once thawed, retain 24 hrs –> Label, kept for 5 days
- Can use plasma immediately
- Prevent plasma waste
- Decreased factor: 5, 7, 8, 10
14
Q
Cryoprecipitate preparation & properties
A
- Prepared by slowing thawing FFP in cold –> Insoluble precipitate: f8, vWF, 13, fibrinogen
- One pool: 4-10 donor units (15mL each) = 1 therapeutic dose
- Content: Fibrinogen > 150mg, F8, vWF, F13
- Storage: -18 up to 1 year, 22C for 6 hours if thawed in closed system (4 hrs if pooled)
15
Q
Leukocyte Reduced RBC
A
- Filter to reduce: FNHTR, Transmission of CMV, Alloimmunization to HLA
- Still have enough T cells to cause TA-GVHD
16
Q
Irradiation
A
- Prevent GVHD in neonate low-birth-weight, intrauterine transfusion, BM transplant, etc.
- Prevent WBC division
- Decrease RBC to 28 days or less
- Used in RBC, PLT, Gran
- Not used for plasma, cryo (freeze kills WBC)
17
Q
Volume Reduction
A
- Reduce plasma volume in cellular products (PLT, WBC)
- Volume sensitive patient (heart dz, renal failure)
- Surrogate for washing
- 1 to 1.5 hrs
- Reduce shelf-life: 24 hr at 1-6C (RBC) and 4hr at 20-24 C (PLT)
18
Q
Washing
A
- Cycle through volume reduction and adding back saline
- For patient with severe reaction to substances in plasma
- Minor mismatch with PLT due to ABO incompatible
- Reduce K for a pediatric patient
- 2hrs+
- Reduce shelf-life: 24 hr at 1-6C (RBC) and 4hr at 20-24 C (PLT)
19
Q
Frozen, Deglycerolized RBC
A
- Apply for rare, antigen negative RBC units
- Preserve up to 10 years in glycerol
- Thawed and washed unit must be used in 24 hours
- 4x cost and 20% additional loss
