BLOOD COLLECTION FROM DONOR

Question 1

SKIN PREPARATION

Answer 1

ASEPTIC METHOD (Alcohol-iodine-alcohol)

Question 2

Substitute for iodine

Answer 2

CHLORHEXEDINE GLUCONATE

Question 3

VOLUME OF BLOOD ROUTINELY COLLECTED

Answer 3

450 mL or 500 mL +/- 10% depending on the collection bag

Question 4

MAXIMUM VOLUME TO BE COLLECTED

Answer 4

10.5 kg/mL including samples for testing

Question 5

LOW VOLUME COLLECTION

Answer 5

300-404 mL in 450 mL bag

333-449 mL in 500 mL bag

Question 6

Total blood volume (blood bag + sample for testing

Answer 6

525 mL

Question 7

DOS and DON’TS (Low volume collection)

Answer 7

RBCs may be transfused

Other components should not be prepared because of the abnormal AC-to-plasma ratio

Question 8

VOLUME OF ANTICOAGULANT

1. 450 mL collection
2. 500 mL collection

Answer 8

1. 63 mL

2. 70 mL

Question 9

AC TO BLOOD RATIO

Answer 9

1:8

Question 10

TIME OF COLLECTION

Answer 10

10-15 minutes (usu. 15mins)

Question 11

If collection time is greater than 15-20mins, unit may not be suitable for prep of:

Answer 11

PLATELETS
FFP
CRYOPRECIPITATED AHF

Question 12

SAMPLES FOR TESTING

Answer 12

Feom diversion pouch or by 2nd phlebotomy

Question 13

DIVERSION POUCH

Answer 13

Collect skin plug

Sample for serologic testing

Question 14

VENIPUNCTURE

Answer 14

Start @ 30-45’ angle then reduce to 10-20’

Question 15

BP cuff pressure

Answer 15

40-60 mmHg