Blood Collection And HgB & Hct

Question 1

PRBC Volume

Answer 1

250-300mL

Question 2

Skin puncture depth for adults and infants

Answer 2

Adults: 2.0 - 2.5 mm
Infants: below 2.0 mm

Question 3

Temperature requirement for microhematocrit method

Answer 3

20 - 24°C

Question 4

EDTA stands for

Answer 4

Ethylenediaminetetraacetic acid or editic acid

Question 5

Band color for heparinized capillary tube

Answer 5

Red coded (nonheparinized are blue coded)

Question 6

Anticoagulant used for arterial blood gas extraction

Answer 6

Heparin

Question 7

How many times the pink stopper is inverted?

Answer 7

8

Question 8

Hemoglobin abnormality

Answer 8

CO2

Question 9

2nd vein choice for M pattern

Answer 9

Accessory Cephalic Vein

Question 10

Blood collection procedures requires

Answer 10

Allen’s test

Question 11

Most common complication during venipuncture that exhibits small blood keaks

Answer 11

Hemolysis

Question 12

Jaundice may exhibit what type of serum?

Answer 12

Icteric (yellow serum)

Question 13

Reagent for hemoglobin determination

Answer 13

DrabKins reagent

Question 14

Type of anticoagulant in light blue stopper

Answer 14

Sodium Citrate

Question 15

Order of draws

Answer 15

Blood culture tube (yellow top)
Coagulation sodium citrate / Citrated tube (light blue top)
Serum w/ or w/o clot activator or gel separator / Serum tube (red/gold top)
Heparin tubes w/ or w/o gel separator / Heparinized tube (green top)
EDTA tube (lavender/pink top)
Glycolytic inhibitor tube / Sodium fluoride tube (gray top)

Question 16

*Number of inversions for each tube

Answer 16

Blood culture, Heparinized, EDTA, & Sodium Fluoride tubes = 8
Citrated & Serum (plastic, clot activator) tubes = 5
Serum (Glass, Non-Additive) tube = 0

Question 17

*3 types of blood collection

Answer 17

Venipuncture
Capillary puncture
Arterial puncture

Question 18

*Ideal site for venipuncture

Answer 18

ANTECUBITAL FOSSA
- median cubital vein
- cephalic / medial cephalic vein
- basilic vein

Question 19

*2 anatomical vein patterns & its choices

Answer 19

H pattern:
1. Median cubital vein
2. Cephalic vein
3. Basilic vein

M pattern:
1. Median vein
2. Accessory cephalic vein
3. Basilic vein

Question 20

• condition in which venous flow is slowed

Answer 20

“stasis”

Question 21

• Hemoglobin a.k.a.

Answer 21

Respiratory pigment

Question 22

• Hemoglobin variants / abnormal hemoglobin pigmentation / dyshemoglobin

Answer 22

1. Methemoglobin
2. Sulfhemoglobin
   3.carboxyhemoglobin

Question 23

• Is the volume of packed RBC that occupies a given volume of blood

Answer 23

Hematocrit (HCT) or Packed Cell Volume