Blood Clot Retraction

Question 1

What happens to the clot when blood coagulation is complete?

Answer 1

When blood coagulation is complete,
the clot normally undergoes RETRACTION

Question 2

_____ is expressed from the clot and
the clot becomes _____

Answer 2

Serum
Denser

Question 3

normal
clot retraction, requires a normal
number of functioning ____,
____, _____, and a normal ____
and ____.

Answer 3

platelets;
calcium;
ATP;
fibrinogen;
fibrin.

Question 4

An abnormal clot retraction time is
found in _______
and _____

Answer 4

Glanzmann’s thrombasthenia;
thrombocytopenia

Question 5

In _____/_____ the formed clot
will be ____and there will be
____amounts of red blood cells
expressed from the clot. (___)

Answer 5

dysfibrinogenemia/hypofibrinogenemia;
small;
increased;
RBC fall out

Question 6

Clot retraction may also be abnormal
in ____

Answer 6

paraproteinemias

Question 7

protein interferes with fibrin formation

Answer 7

paraproteinemias

Question 8

In ____,
the formed ____ will appear ___ and
____, and increased_____.

Answer 8

DIC;
clot;
small;
ragged;
RBC fall out

Question 9

T/F: In blood containing high red blood
cells count, the degree of retraction is
limited became of the large volume of
RBC within the clot

Answer 9

T

Question 10

In blood containing high red blood
cells count, the degree of _____is
_____ became of the large volume of
___ within the ____.

In ___states,
the ___ occurs and the degree of
clot retraction is ___

Answer 10

retraction;
limited;
RBC;
clot

anemia;
reverse;
increased

Question 11

METHODS OF BLOOD RETRACTION

Answer 11

1. Single Tube Method
2. Hirshboeck Method/Castor Oil Method

Question 12

A. SINGLE TUBE METHOD

Procedure
1. Place ____ ____ in a clean dry
____tube.
2. ___ a coiled wire in the ____ of the
tube (____ thick, with a ___diameter
coil)
3. Incubate at ____ for ____ after
clotting has occurred.
4. Gently lift the ___ and allow the
attached ___ to drain for ____.
5. Read the volume of fluid ___ in
the tube. Expressed this volume as a
____ of the original volume of
the whole blood placed in the tube.

Answer 12

1. 5 ml; venous blood; centrifuge
2. Insert; bottom of the tube (1mm; cm)
3. 37oC; 1 hour
4. wire ; clot ; 2 minutes.
5. remaining; percentage

Question 13

ARRANGE THE FF PROCEDURE OF SINGLE TUBE METHOD IN ORDER:

Read the volume of fluid remaining in
the tube. Expressed this volume as a
percentage of the original volume of
the whole blood placed in the tube.

Insert a coiled wire in the bottom of the
tube (1 mm thick, with a cm diameter
coil)

Place 5 ml venous blood in a clean dry
centrifuge tube.

Gently lift the wire and allow the
attached clot to drain for 2 minutes.

Incubate at 37oc for 1 hour after
clotting has occurred.

Answer 13

1. Place 5 ml venous blood in a clean dry
   centrifuge tube.
2. Insert a coiled wire in the bottom of the
   tube (1 mm thick, with a cm diameter
   coil)
3. Incubate at 37oc for 1 hour after
   clotting has occurred.
4. Gently lift the wire and allow the
   attached clot to drain for 2 minutes.
5. Read the volume of fluid remaining in
   the tube. Expressed this volume as a
   percentage of the original volume of
   the whole blood placed in the tube.

Question 14

Reference Range in SINGLE TUBE METHOD

Answer 14

44-67%

Question 15

Color and Character of Serum
1. Normally, a ____serum
appears within the ____ hour. In ____
hours, the volume varies from ____ to
_____ of the total amount of blood.

Answer 15

clear pale yellow ;
first ;
18- 24 hours;
40%;
57%

Question 16

2. When the clot is ___and
   ___of ____, the volume of serum is less than ____

Answer 16

defective
incapable
proper retraction,
40%.

Question 17

Serum may appear ___ after ___
or in ___and ____.

Answer 17

milky
meals
diabetes
leukemia

Question 18

may be deep yellow in haemolytic
jaundice leukemia, obstructive
jaundice or in carotenemia.

Answer 18

Serum

Question 19

Serum may be ___ in ____, ____
or in _____.

Answer 19

deep yellow;
haemolytic jaundice leukemia;
obstructive jaundice;
carotenemia

Question 20

Materials used in HIRSHBOECK METHOD

Answer 20

● Castor oil
● WBC diluting pipette

Question 21

Procedure (HIRSHBOECK METHOD)
1. Puncture sterilized ___, ____off ___
drop, suck ____ of blood in a ____.

Answer 21

finger;
wipe;
first;
20 ml;
Sahli’s pipette.

Question 22

Procedure (HIRSHBOECK METHOD)

2. Slowly, allow the ____ to flow down
   the pipette until a ____ drop
   accumulates at the ____.

Answer 22

blood;
big;
tip

Question 23

Procedure (HIRSHBOECK METHOD)

3. Slowly ___ the pipette until the drop
   of blood touches the ____, and
   allow the blood to be ___ just
   ___the _____.

Answer 23

lower;
castor oil;
suspended;
below;
meniscus.

Question 24

Procedure (HIRSHBOECK METHOD)

4. Start the ____ and observe for a
   visible ___ or a ____
   protrusion on the surface of the drop of
   blood. Note the ___.

Answer 24

timing;
dimpling;
nipple-like;
time

Question 25

This indicates
the end front of the test

Answer 25

time

Question 26

T/F clot retraction is reported delayed if
dimpling happens after 2 hour.

Answer 26

f; 1 hr

Question 27

CLINICAL SIGNIFICANCEOF HIRSHBOECK METHOD

Answer 27

Platelet disorders and
thrombocytopenia

Question 28

process wherein the serum is expressed from the clot and the clot becomes denser

Answer 28

retraction