Blood Clot Retraction Flashcards
What happens to the clot when blood coagulation is complete?
When blood coagulation is complete,
the clot normally undergoes RETRACTION
_____ is expressed from the clot and
the clot becomes _____
Serum
Denser
normal
clot retraction, requires a normal
number of functioning ____,
____, _____, and a normal ____
and ____.
platelets;
calcium;
ATP;
fibrinogen;
fibrin.
An abnormal clot retraction time is
found in _______
and _____
Glanzmann’s thrombasthenia;
thrombocytopenia
In _____/_____ the formed clot
will be ____and there will be
____amounts of red blood cells
expressed from the clot. (___)
dysfibrinogenemia/hypofibrinogenemia;
small;
increased;
RBC fall out
Clot retraction may also be abnormal
in ____
paraproteinemias
protein interferes with fibrin formation
paraproteinemias
In ____,
the formed ____ will appear ___ and
____, and increased_____.
DIC;
clot;
small;
ragged;
RBC fall out
T/F: In blood containing high red blood
cells count, the degree of retraction is
limited became of the large volume of
RBC within the clot
T
In blood containing high red blood
cells count, the degree of _____is
_____ became of the large volume of
___ within the ____.
In ___states,
the ___ occurs and the degree of
clot retraction is ___
retraction;
limited;
RBC;
clot
anemia;
reverse;
increased
METHODS OF BLOOD RETRACTION
- Single Tube Method
- Hirshboeck Method/Castor Oil Method
A. SINGLE TUBE METHOD
Procedure
1. Place ____ ____ in a clean dry
____tube.
2. ___ a coiled wire in the ____ of the
tube (____ thick, with a ___diameter
coil)
3. Incubate at ____ for ____ after
clotting has occurred.
4. Gently lift the ___ and allow the
attached ___ to drain for ____.
5. Read the volume of fluid ___ in
the tube. Expressed this volume as a
____ of the original volume of
the whole blood placed in the tube.
- 5 ml; venous blood; centrifuge
- Insert; bottom of the tube (1mm; cm)
- 37oC; 1 hour
- wire ; clot ; 2 minutes.
- remaining; percentage
ARRANGE THE FF PROCEDURE OF SINGLE TUBE METHOD IN ORDER:
Read the volume of fluid remaining in
the tube. Expressed this volume as a
percentage of the original volume of
the whole blood placed in the tube.
Insert a coiled wire in the bottom of the
tube (1 mm thick, with a cm diameter
coil)
Place 5 ml venous blood in a clean dry
centrifuge tube.
Gently lift the wire and allow the
attached clot to drain for 2 minutes.
Incubate at 37oc for 1 hour after
clotting has occurred.
- Place 5 ml venous blood in a clean dry
centrifuge tube. - Insert a coiled wire in the bottom of the
tube (1 mm thick, with a cm diameter
coil) - Incubate at 37oc for 1 hour after
clotting has occurred. - Gently lift the wire and allow the
attached clot to drain for 2 minutes. - Read the volume of fluid remaining in
the tube. Expressed this volume as a
percentage of the original volume of
the whole blood placed in the tube.
Reference Range in SINGLE TUBE METHOD
44-67%
Color and Character of Serum
1. Normally, a ____serum
appears within the ____ hour. In ____
hours, the volume varies from ____ to
_____ of the total amount of blood.
clear pale yellow ;
first ;
18- 24 hours;
40%;
57%
- When the clot is ___and
___of ____, the volume of serum is less than ____
defective
incapable
proper retraction,
40%.
Serum may appear ___ after ___
or in ___and ____.
milky
meals
diabetes
leukemia
may be deep yellow in haemolytic
jaundice leukemia, obstructive
jaundice or in carotenemia.
Serum
Serum may be ___ in ____, ____
or in _____.
deep yellow;
haemolytic jaundice leukemia;
obstructive jaundice;
carotenemia
Materials used in HIRSHBOECK METHOD
● Castor oil
● WBC diluting pipette
Procedure (HIRSHBOECK METHOD)
1. Puncture sterilized ___, ____off ___
drop, suck ____ of blood in a ____.
finger;
wipe;
first;
20 ml;
Sahli’s pipette.
Procedure (HIRSHBOECK METHOD)
- Slowly, allow the ____ to flow down
the pipette until a ____ drop
accumulates at the ____.
blood;
big;
tip
Procedure (HIRSHBOECK METHOD)
- Slowly ___ the pipette until the drop
of blood touches the ____, and
allow the blood to be ___ just
___the _____.
lower;
castor oil;
suspended;
below;
meniscus.
Procedure (HIRSHBOECK METHOD)
- Start the ____ and observe for a
visible ___ or a ____
protrusion on the surface of the drop of
blood. Note the ___.
timing;
dimpling;
nipple-like;
time
