Blood Cell Cytochemistry Flashcards
Best AML prognosis
M2: Myeloblasts with Maturation
Sudan Black B is a test for
Lipids
Periodic Acid Schiff is a test for
Glycogen
Specific stain for esterase
Naphthol AS-D Choloroacetate esterase
Non-specific stain for esterase
a-naphthyl acetate esterase
end color of PAS
red color/bright pink
Interpretation of grading 2+
Pale with moderate amount of blue staining
Interpretation of grading 1+
Faint and diffuse staining
Interpretation of grading 4+
Deep blue or brilliant staining with no visible cytoplasm
Interpretation of grading 3+
Strong blue precipitated staining
Diagnostic tool for confirmation of hairy cell leukemia
TRAP
Rarest AML
M7: Megakaryocytic Leukemia
Enzymatic Cytochemical stains
MPO, Esterase, TRAP, LAP
Non enzymatic Cytochemical stains
Sudan Black B, PAS, Toluidine Blue, Prussian Blue, Immunocytochemical
Fixative of LAP
Citrate
Buffer of LAP
Acetone
Positive result of toluidine blue and color
Metachromatic granules - reddish violet
Most common childhood leukemia
L1: small lymphoblast
Substrate of myeloperoxidase stain
H2O2 + 3-Amino-9-Ethyl carbazole
Positive in megakaryoblastic leukemia
Factor VIII antibodies
Differentiates CML from leukemoid reaction
LAP stain
Specific esterase is a test for
Granulocytic precursors
Non-specific esterase is a test for
Monocyte precursors
Substrate of PAS
Dialdehyde
Differentiates basophilic leukemia and mast cell leukemia from other diseases
Toluidine Blue
Component/reaction of Perl’s Stain
Ferricyanide + HC (positive)
