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Blood Cell Cytochemistry > Blood Cell Cytochemistry > Flashcards

Blood Cell Cytochemistry Flashcards

1
Q

Best AML prognosis

A

M2: Myeloblasts with Maturation

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2
Q

Sudan Black B is a test for

A

Lipids

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3
Q

Periodic Acid Schiff is a test for

A

Glycogen

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4
Q

Specific stain for esterase

A

Naphthol AS-D Choloroacetate esterase

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5
Q

Non-specific stain for esterase

A

a-naphthyl acetate esterase

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6
Q

end color of PAS

A

red color/bright pink

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7
Q

Interpretation of grading 2+

A

Pale with moderate amount of blue staining

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8
Q

Interpretation of grading 1+

A

Faint and diffuse staining

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9
Q

Interpretation of grading 4+

A

Deep blue or brilliant staining with no visible cytoplasm

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10
Q

Interpretation of grading 3+

A

Strong blue precipitated staining

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11
Q

Diagnostic tool for confirmation of hairy cell leukemia

A

TRAP

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12
Q

Rarest AML

A

M7: Megakaryocytic Leukemia

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13
Q

Enzymatic Cytochemical stains

A

MPO, Esterase, TRAP, LAP

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14
Q

Non enzymatic Cytochemical stains

A

Sudan Black B, PAS, Toluidine Blue, Prussian Blue, Immunocytochemical

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15
Q

Fixative of LAP

A

Citrate

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16
Q

Buffer of LAP

A

Acetone

17
Q

Positive result of toluidine blue and color

A

Metachromatic granules - reddish violet

18
Q

Most common childhood leukemia

A

L1: small lymphoblast

19
Q

Substrate of myeloperoxidase stain

A

H2O2 + 3-Amino-9-Ethyl carbazole

20
Q

Positive in megakaryoblastic leukemia

A

Factor VIII antibodies

21
Q

Differentiates CML from leukemoid reaction

A

LAP stain

22
Q

Specific esterase is a test for

A

Granulocytic precursors

23
Q

Non-specific esterase is a test for

A

Monocyte precursors

24
Q

Substrate of PAS

A

Dialdehyde

25
Q

Differentiates basophilic leukemia and mast cell leukemia from other diseases

A

Toluidine Blue

26
Q

Component/reaction of Perl’s Stain

A

Ferricyanide + HC (positive)

Blood Cell Cytochemistry (1 decks)

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