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MLS(ASCP) - Blood Bank > Blood Bank > Flashcards

Blood Bank Flashcards

1
Q

At what temperature is PF24 or FFP thawed for transfusion?

A

37oC

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2
Q

What is the expiration date of liquid plasma?

A

5 days after expiration of the whole blood unit from which it was collected

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3
Q

What is the expiration of irradiated pRBCs?

A

28 days or original expiration, whichever occurs first.

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4
Q

What is the expiration of frozen red cells?

A

10 years

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5
Q

At what temperature are granulocytes stored?

A

20 - 24oC

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6
Q

What is the blood donation deferral period for receiving measles, mumps, or yellow fever vaccine?

A

2 weeks

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7
Q

What is the blood donation deferral period for receiving a tattoo?

A

12 months

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8
Q

What is the blood donation deferral period for receiving a stem cell transplant?

A

12 months

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9
Q

What is the minimum hemoglobin/hematocrit required to donate whole blood?

A

Hemoglobin = > 12.5 g/dL

Hematocrit = > 38%

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10
Q

What is the blood donation deferral period for a female who had a sexual encounter with a male who had sexual contact with another male?

A

12 months

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11
Q

What is the blood donation deferral for persons who was/currently diagnosed with leukemia?

A

Indefinite deferral

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12
Q

What is the minimum weight required for whole blood donation?

A

110 lbs

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13
Q

How long must donor records be retained by the collection facility?

A

10 years

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14
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

Autosomal Dominant

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15
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

Autosomal Recessive

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16
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

X-linked Dominant

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17
Q

Refer to the illustration below.

Can you identify the inheritance pattern of this pedigree?

A

X-linked Recessive

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18
Q

What lectin is used to agglutinate A1 or A1B cells?

A

Dolichos biflorus

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19
Q

Which lectin is used to agglutinate Group O red cells?

A

Ulex europaeus

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20
Q

Which lectin is used to agglutinate Group B cells?

A

Bandeiraea simplicifolia

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21
Q

What is the Rh phenotype of an individual who inherits genotype R1r?

A

See below:

22
Q

What is the Rh phenotype of an individual who inherits genotype R2R2?

A

See table below:

23
Q

What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

A

rr

24
Q

What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

A

R1R2

25
Q

What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:

A

Rh: 1, 2, -3, -4, 5

26
Q

What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:

A

Rh: 1, -2, -3, 4, 5

27
Q

What is the most probable genotype using the Fisher-Race nomenclature for the following phenotype: R2r

A

cDE/ce

28
Q

If transfused, which Rh antibody(ies) would you expect a Ror individual to produce?

A
  • Anti-C
  • Anti-E
29
Q

What test is performed to calculate the amount of fetal red cells entering the maternal circulation during a hemorrhage event?

A

Kleihaeur-Betke acid elution test

30
Q

How much whole blood and/or fetal red cells will a standard dose of Rh Immune globulin cover?

A

15 mL of packed cells or 30 mL of whole blood

31
Q

List three ways in which you can inherit the Lu(a-b-) phenotype:

A
  1. Homozygous inheritance of the recessive LuLu gene (Recessive)
  2. Inheritance of an X-linked recessive gene GATA-1 (X-linked recessive)
  3. Inheritance of a mutated, dominant KLFI gene (Dominant inhibitor)
32
Q

List 2 instances when a Dweak test is performed:

A
  1. All donors who initial are immediate spin Rh negative
  2. Rh negative pregnant females
33
Q

What is the ABO Group of antibody screening cells?

A

Group O

34
Q

Using the Weiner nomenclature, what is the Rh genotype of screening cells: SC-I, SC-II, and SC-III?

A
  • SC-I = R1R1
  • SC-II = R2R2
  • SC-III = rr
35
Q

When performing an Indirect Antiglobulin Test (IAT) antibody attachment is observed in which phases?

A

Immediate Spin (optional)

37oC

AHG (antihuman globulin)

36
Q

Refer to the table below:

List 3 actions you could perform to resolve this ABO discrepancy:

A
  1. Check the age of the patient
  2. Incubate reverse group for 30 minutes (do not perform this on a newborn or cord blood sample)
  3. Incubate reverse group at 4oC and incubate for 15 minutes. (Use screening cells as a control)

Patient is probably Group O. The steps above will enhance plasma agglutination.

37
Q

Refer to the table below:

List 3 actions you could do to resolve this ABO discrepancy:

A
  1. Acidify anti-B reagent to pH = 6
  2. Run DAT
  3. Run autocontrol

Patient is most likely Group A. Reaction with anti-B is due to Acquired B-like antigen seen in patients with colorectal cancer or other GI tract conditions.

38
Q

Refer to the table below:

List 3 actions you could perform to resolve this ABO discrepancy:

A
  1. Perform microscopic examination of the reverse group testing
  2. Perform saline replacement technique
  3. Perform IAT
39
Q

Refer to the table below.

How would you interpret this antibody screen?

A

Positive IAT

40
Q

Refer to the table below.

How would you interpret this antibody screen?

A

Invalid IAT

Must repeat test

41
Q

Refer to the table below.

How would you interpret this antibody screen?

A

Positive IAT

42
Q

Refer to the table below.

How would you interpret this antibody screen?

A

Negative IAT

43
Q

Interpret the DAT panel below.

Should you perform an eluate on the sample?

A

DAT = Positive for complement only

No eluate indicated

44
Q

Interpret the DAT panel below.

Should you perform an eluate on the sample?

A

DAT = Positive for IgG and complement

Perform eluate

45
Q

Interpret the DAT panel below.

Should you perform an eluate on the sample?

A

Invalid - DAT panel should be repeated

Do not perform eluate until poly AHG result is confirmed

46
Q

List 3 blood group systems in which antigens are sensitive to papain/ficin treatment?

A
  1. Duffy
  2. MN
  3. Xg
47
Q

List at least 3 blood group systems in which antigens are resistant to papain/ficin treatment?

A
  1. Rh
  2. Kidd
  3. S, s, U
  4. Kell
  5. Lewis
  6. P
  7. Lutheran
48
Q

List 3 solutions used to enhance agglutination in IAT?

A
  1. PEG
  2. LISS
  3. 22% Albumin
49
Q

List 4 methods used to enhance antihuman globulin tests:

A
  1. Increase incubation time
  2. Increase temperature
  3. Washing red cells
  4. Increase serum:cell ratio
50
Q

Donath-Landstiener antibody belongs to which blood group system?

A

P Blood Group

51
Q

List 2 FDA approved methods used to automate blood bank serological testing:

A
  1. Gel technology
  2. Solid phase technology
52
Q

How many vials of RhIg should a Rh(-) pregnant woman receive if the number of fetal cells determined by K-B test = 3.6%

A

7 vials of RhIg

MLS(ASCP) - Blood Bank (1 decks)

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