Blood Bank Flashcards
At what temperature is PF24 or FFP thawed for transfusion?
37oC
What is the expiration date of liquid plasma?
5 days after expiration of the whole blood unit from which it was collected
What is the expiration of irradiated pRBCs?
28 days or original expiration, whichever occurs first.
What is the expiration of frozen red cells?
10 years
At what temperature are granulocytes stored?
20 - 24oC
What is the blood donation deferral period for receiving measles, mumps, or yellow fever vaccine?
2 weeks
What is the blood donation deferral period for receiving a tattoo?
12 months
What is the blood donation deferral period for receiving a stem cell transplant?
12 months
What is the minimum hemoglobin/hematocrit required to donate whole blood?
Hemoglobin = > 12.5 g/dL
Hematocrit = > 38%
What is the blood donation deferral period for a female who had a sexual encounter with a male who had sexual contact with another male?
12 months
What is the blood donation deferral for persons who was/currently diagnosed with leukemia?
Indefinite deferral
What is the minimum weight required for whole blood donation?
110 lbs
How long must donor records be retained by the collection facility?
10 years
Refer to the illustration below.
Can you identify the inheritance pattern of this pedigree?

Autosomal Dominant
Refer to the illustration below.
Can you identify the inheritance pattern of this pedigree?
Autosomal Recessive
Refer to the illustration below.
Can you identify the inheritance pattern of this pedigree?
X-linked Dominant
Refer to the illustration below.
Can you identify the inheritance pattern of this pedigree?
X-linked Recessive
What lectin is used to agglutinate A1 or A1B cells?
Dolichos biflorus
Which lectin is used to agglutinate Group O red cells?
Ulex europaeus
Which lectin is used to agglutinate Group B cells?
Bandeiraea simplicifolia
What is the Rh phenotype of an individual who inherits genotype R1r?
See below:

What is the Rh phenotype of an individual who inherits genotype R2R2?

See table below:
What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

rr
What is the most probable genotype using the Weiner nomenclature for the following phenotype illustrated below:

R1R2
What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:

Rh: 1, 2, -3, -4, 5
What is the most probable genotype using the Rosenfield nomenclature for the following phenotype illustrated below:

Rh: 1, -2, -3, 4, 5
What is the most probable genotype using the Fisher-Race nomenclature for the following phenotype: R2r
cDE/ce
If transfused, which Rh antibody(ies) would you expect a Ror individual to produce?
- Anti-C
- Anti-E
What test is performed to calculate the amount of fetal red cells entering the maternal circulation during a hemorrhage event?
Kleihaeur-Betke acid elution test
How much whole blood and/or fetal red cells will a standard dose of Rh Immune globulin cover?
15 mL of packed cells or 30 mL of whole blood
List three ways in which you can inherit the Lu(a-b-) phenotype:
- Homozygous inheritance of the recessive LuLu gene (Recessive)
- Inheritance of an X-linked recessive gene GATA-1 (X-linked recessive)
- Inheritance of a mutated, dominant KLFI gene (Dominant inhibitor)
List 2 instances when a Dweak test is performed:
- All donors who initial are immediate spin Rh negative
- Rh negative pregnant females
What is the ABO Group of antibody screening cells?
Group O
Using the Weiner nomenclature, what is the Rh genotype of screening cells: SC-I, SC-II, and SC-III?
- SC-I = R1R1
- SC-II = R2R2
- SC-III = rr
When performing an Indirect Antiglobulin Test (IAT) antibody attachment is observed in which phases?
Immediate Spin (optional)
37oC
AHG (antihuman globulin)
Refer to the table below:
List 3 actions you could perform to resolve this ABO discrepancy:

- Check the age of the patient
- Incubate reverse group for 30 minutes (do not perform this on a newborn or cord blood sample)
- Incubate reverse group at 4oC and incubate for 15 minutes. (Use screening cells as a control)
Patient is probably Group O. The steps above will enhance plasma agglutination.
Refer to the table below:
List 3 actions you could do to resolve this ABO discrepancy:

- Acidify anti-B reagent to pH = 6
- Run DAT
- Run autocontrol
Patient is most likely Group A. Reaction with anti-B is due to Acquired B-like antigen seen in patients with colorectal cancer or other GI tract conditions.
Refer to the table below:
List 3 actions you could perform to resolve this ABO discrepancy:

- Perform microscopic examination of the reverse group testing
- Perform saline replacement technique
- Perform IAT
Refer to the table below.
How would you interpret this antibody screen?

Positive IAT
Refer to the table below.
How would you interpret this antibody screen?

Invalid IAT
Must repeat test
Refer to the table below.
How would you interpret this antibody screen?

Positive IAT
Refer to the table below.
How would you interpret this antibody screen?

Negative IAT
Interpret the DAT panel below.
Should you perform an eluate on the sample?

DAT = Positive for complement only
No eluate indicated
Interpret the DAT panel below.
Should you perform an eluate on the sample?

DAT = Positive for IgG and complement
Perform eluate
Interpret the DAT panel below.
Should you perform an eluate on the sample?

Invalid - DAT panel should be repeated
Do not perform eluate until poly AHG result is confirmed
List 3 blood group systems in which antigens are sensitive to papain/ficin treatment?
- Duffy
- MN
- Xg
List at least 3 blood group systems in which antigens are resistant to papain/ficin treatment?
- Rh
- Kidd
- S, s, U
- Kell
- Lewis
- P
- Lutheran
List 3 solutions used to enhance agglutination in IAT?
- PEG
- LISS
- 22% Albumin
List 4 methods used to enhance antihuman globulin tests:
- Increase incubation time
- Increase temperature
- Washing red cells
- Increase serum:cell ratio
Donath-Landstiener antibody belongs to which blood group system?
P Blood Group
List 2 FDA approved methods used to automate blood bank serological testing:
- Gel technology
- Solid phase technology
How many vials of RhIg should a Rh(-) pregnant woman receive if the number of fetal cells determined by K-B test = 3.6%
7 vials of RhIg