Blood Bank 4

Question 1

S and s code for the production of which siaglycoprotein?

Answer 1

Glycophorein B

Question 2

All cells that are positive for either S or s are also positive for what antigen?

Answer 2

U

Question 3

What temp doe anti-M most commonly react at?

Answer 3

Room temp or below

Question 4

Anti-M and anti-N characteristics

Answer 4

Exhibit dosage

Inhibited by enzymes

Question 5

Anti-S and anti-s react at what temp

Answer 5

37C and AHG

Question 6

Does enzyme treatment effect reactivity of anti-S or -s

Answer 6

Variable effect

Question 7

Is P1 or P2 more common

Answer 7

P1

Question 8

What antigens do P1 individuals have

Answer 8

P1
P
Pk

Question 9

What antigens do P2 individuals have

Answer 9

P

Pk

Question 10

Which alloantibody, -P or -P1 is more significant for transfusion purposes

Answer 10

Anti-P

Question 11

What autoantibody is known as Donath-Landsteiner antibody

Answer 11

Autoanti-P

Question 12

How does Donath-Landsteiner Ab cause intravascular hemolysis

Answer 12

-P binds to RBCs at lower temps in peripheral circulation, activates complement which causes intravascular hemolysis when warmed back to 37C in body core

Question 13

What disease is Donath-Landsteiner Ab associated with

Answer 13

Paroxysmal cold hemoglobinuria

Question 14

From what is P1 substance made and what does it do

Answer 14

Pigeon egg whites

Inhibits P1 antibody reactions

Question 15

What what temp does anti-LuA and what type of reactivity is seen

Answer 15

IS/room temp

Mf pattern of small agglutinates in background of free cells

Question 16

At what phase does anti-LuB react

Answer 16

AHG and 37C

Question 17

Is LuA high or low frequency

Answer 17

Low

Question 18

Is LuB antigen high or low frequency

Answer 18

High

Question 19

Most significant high frequency Kell antigen

Answer 19

k
KpB
JsB

Question 20

What are the most significant low frequency Kell antigens

Answer 20

K
KpA
JsA

Question 21

McLeod phenotype characteristics

Answer 21

Absence of normal XK1 gene
Kx Ag not produced
Kell antigens decreased in amount

Question 22

McLeod syndrome sometimes associated with what immunodeficiency

Answer 22

Type 2 chronic granulomatous disease

Question 23

K0 phenotype have what antigens

Answer 23

High level Kx

Lack all other Kell antigens

Question 24

Antigen second to D in immunogenicity

Answer 24

Kell

Question 25

At what phase do K system antigen show up

Answer 25

AHG only

Question 26

What genes influence Lewis phenotype

Answer 26

Le | Se

Question 27

Effect of pregnancy on Lewis antigens

Answer 27

Can cause transient decline

Question 28

Lewis phenotype of leleSese individual

Answer 28

Le(a-b-) | No expression

Question 29

Lewis phenotype of Lelesese individual

Answer 29

Le (a+b+) | LeA only

Question 30

Lewis phenotype of LeleSese individual

Answer 30

Le (a-b+) | LeB only

Question 31

Most common Lewis phenotype

Answer 31

LeB

Question 32

Are Lewis antibodies commonly NRCS

Answer 32

Yes

Question 33

Why do Lewis antibodies not commonly cause HDFN?

Answer 33

Usually IgM Rarely IgG Antigens not well developed at birth

Question 34

What Duffy phenotype are most whites

Answer 34

FyAB (a+b+)

Question 35

What Duffy phenotype are most blacks

Answer 35

Fy (a-b-)

Question 36

Cells with Fy (a-b-) phenotype are resistant to what

Answer 36

Plasmodium vivax infection

Question 37

At what phase do Duffy system antibodies show up

Answer 37

AHG

Question 38

What does enzyme treatment of cells do to reactivity of Fy antibodies

Answer 38

Terminated reactivity

Question 39

At what phase do Kidd antibodies show up

Answer 39

AHG

Question 40

What activity of Kidd allows for detection

Answer 40

Complement binding activity | Causes hemolysis

Question 41

Most common Kidd phenotype in whites

Answer 41

Jk (a+b+)

Question 42

Most common Kidd phenotype in blacks

Answer 42

Jk (a+b-)

Question 43

What trait of Kidd antibodies make them likely to cause HTR

Answer 43

Disappearing act, levels quickly fall to undetectable levels

Question 44

Is I activity stronger in adult or newborn

Answer 44

Adult

Question 45

Is i activity stronger in adult or newborn

Answer 45

Newborn

Question 46

What type of adult has weak expression of I activity and strong expression of i activity and may develop a potent alloanti-I

Answer 46

ii phenotype seen in Japanese individuals

Question 47

Potent autoanti-i capable of causing transient hemolytic anemia is often associated with what disease

Answer 47

Infectious mono

Question 48

What precautions should be taken when transfusing a patient with a potent anti-I or -i

Answer 48

Blood warmer Slower transfusion Keep patient warm

Question 49

What cold reactive alloantibody can group A individuals make which will cause serum to react with all group O cells, but not with group A cells

Answer 49

Anti-IH Must use pre warmed technique Characteristically clumps at IS then goes away

Question 50

What antibodies are inactivated by enzyme treatment

Answer 50

XgA Duffy (Fy) M N

Question 51

Characteristics of anti-XgA

Answer 51

``` Doesn't usually cause HDFN 66% of males 89% of females Usually immune stimulated IgG Detected at AHG Binds complement Doesn't cause in vitro hemolysis ```

Question 52

Type of reactivity seen with anti-SdA

Answer 52

Mixed field pattern of small tightly agglutinated refractile clumps in background of free cells at AHG

Question 53

What can be used to neutralize anti-SdA

Answer 53

SdA substance found in urine

Question 54

Which blood group shows a relationship with the HLA antigens found on young nucleated RBCs

Answer 54

Bg

Question 55

HTLA stands for what

Answer 55

High titer low avidity

Question 56

Chief features of HTLA antibodies

Answer 56

Typically demonstrate weak reactivity that persists even when sera is significantly diluted, Pattern of reactivity at AHG often not reproducible, IgG antibodies may be immune or naturally occurring

Question 57

M and N code for production of which sialoglycoprotein?

Answer 57

Glycophorin A