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BOC study > blood bank > Flashcards

blood bank Flashcards

1
Q

Donor requirements

A

> =16 yo
=8wks single unit whole blood
=16 wks double unit blood
temp: <=99.5
BP: 90-180/50-100 mmHg
pulse: 50-100bpm
110lbs
H/H: >=12.5g/dL/38% W; >=13g/dL/39% M

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2
Q

Autologous donorreqs

A

physicians order
H/H: >=11g/dL/33%
collection >72H before surgery
no risk of bacteremia
Confirm ABO/Rh

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3
Q

Directed donation

A

Good for low incidence antigens

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4
Q

Drug deferals
Tegison

A

Permenant

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5
Q

drug deferals
Erivedge
odomzo
aubagio
arava

A

24 months

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6
Q

Drug deferals
proscar, propecia
accutane
avodart, Jalyn
soriatane
Cellcept

A

1 mo
1mo
6 mo
3 y
6 w

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7
Q

Dru deferal
Aspirin

A

2 days

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8
Q

Drug deferal
plavix

A

14 days

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9
Q

Drug deferal
Warfarin

A

For plasma, 7d

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10
Q

drug deferal
HIV prevention

A

3m

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11
Q

drug deferal
HIV prevention (injectable!)

A

2y

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12
Q

Apheresis Donor Req

A

Plasma:
once every 4 wks OR <= 2/wk at least 2 days apart
>=50kg
Platelets:
2 day donation interval
once a wk for double/tripple donation
<= 24 donation in 12 mo
count >= 150 X10^9

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13
Q

Donation collection

A

Aseptic technique
NO green soap
iodione or iodine and iso alc
10-40 ml diverted to avoid contamination
MAX: 10.5 mL/kg of donor weight

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14
Q

donor adverse reactions

A

most occur at the site
Vasovagal (pass out)
injury from needle insertion
Citrate toxicity, air embolism, hypovolemia
allergic reactions (usually iodine)

Post-donation Fe deficiency

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15
Q

Donor serological testing

A

ABO
Rh
Weak D testing if Rh =

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16
Q

Donor disease testing

A

Every donation screened every time, except Cruzi which is a 1 time
Babesia dependant on location

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17
Q

donor plt testing

A

10-40mL diverted for testing, can contain contaminated skin
Cultures maintained for the shelf life of the unit
issued after the first 12 h of incubation

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18
Q

Blood component manufacture

A

WB to plateletes: cooled to 20-24C or units to 1-10C
cetrifuged for shortest time and lowest speed. reduces dmg

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19
Q

red blood cell products

A

42 day shelf life
2,3 DPG declines rapidly after 1-2wks storage,
transfusion for babies usually less that a week old because of 2,3 DPG

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20
Q

FFP product

A

placed in freeze <=-18C within 8hrs
PF24 is not frozen w/in 8 hrs but before 24 h. 1-6C w/in 8 hrs

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21
Q

Cryoprecipitate product

A

FFP that is thawed @1-6C
white material that is suspended in thawed plasma
resuspended in 10-15 mL plasma and frozen
pooled: 4-10 units into single unit, then frozen

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22
Q

plateletes products

A

low spin to seperate RBC from PRP
hard spin to seperate plateletes from plasma
plasma removed and pellet resuspended
Pooled: 5 day expiration from oldest unit
pooled before transfusion 4h expiration
adult transfusion 4-6 units

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23
Q

expiration of bacterial risk control
Pathogen reduction

A

5 days

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24
Q

Expiration for bacterial control
LVDS 36h

A

5days

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25
Q

Expiration for bacterial control
LVDS 48h

A

7days

26
Q

Expiration for bacterial control
pri culture 24h; 2nd culture 3 days

A

5 days

27
Q

Expiration for bacterial control
pri culture 36h; 2nd culture 4 days

A

7 days

28
Q

Expiration for bacterial control
pri culture 36h; 2nd culture rapid detection

A

7 days

29
Q

Expiration for bacterial control
rapid bacterial testing

A

WBD platelets: 5 days
post storage pools WBDP: 4 h

30
Q

Expiration for bacterial control
Single culture sampling 36h after collection

A

5 days

31
Q

Expiration for bacterial control
single culture sample 24h after collection

A

5 days

32
Q

Pathogen reduction technology

A

Amonosalen+UVA light: prevents nucleic acid replication
platelets that undergo are not required to be cultured
alternative for babesia questioning
inactivate WBCs, removing risk of GVHD and negating irradiation

33
Q

Sterile connection device

A

closed system maintains original expiration date
uses: prestorage pooled platelete/pooled cryo AHF
Splitting units for fetal use, attachment for LR filters, culture sampling

34
Q

storage and transport

A

temp recorded and logged every 4h at least
RBC: 1-6C/transport 1-10C
frozen RBC:<= -65C/transport maintain frozen
platelete: 20-24C w/ agitation/transport 20-24C, <=30h w/o agitation
cold store platelets: 1-6C, optional agitation/transport 1-10C
FFP/Cryo: <=-18C/transport maintain frozen
thawed plasma:1-6C/ transport 1-10C
Thawed cryo: 20-24C/same
Aphoresis gran: 20-24C/same

35
Q

shelf life
LRWB and WB

A

CPD/CP2D: 21d
CPDA-1: 35D
Open system 24h

36
Q

Shelf life
RBC

A

42d

37
Q

Shelf life
LRBC

A

42d

38
Q

Shelf life
Apheresis RBC

A

42d

39
Q

Shelf life
Frozen RBCs

A

10y

40
Q

Shelf life
deglycerolyzed RBCs

A

open system: 24h
Closed system: 14d

41
Q

Shelf life
Plateletes

A

5d

42
Q

shelf life
pooled platelets

A

5d, up to 7d with rapid bacterial testing

43
Q

shelf life
apheresis platelets

A

5d-7d

44
Q

shelf life
apheresis platelets (patho reduced)

A

5d

45
Q

shelf life
aphoresis platelets additive solution

A

5d

46
Q

shelf life
cold store platelets

A

14d at 1-6C

47
Q

shelf life aphoreseis granulocytes

A

24h

48
Q

shelf life FFP

A

<= -18C: 12m
<=-65C: 7y

49
Q

shelf life PF24 plasma

A

<= -18C: 12m

50
Q

shelf life
Thawed plasma

A

4d after initial 24h thaw period

51
Q

shelf life
Cryo

A

<= -18C: 12m
non-pooled: 6h thawed
Pooled: 4h thawed

52
Q

blood product QC
RBC

A

RBC: HCT >= 80%
LRBC: <5x10^6 Leuk
Apheresis RBC: >= 60g Hb
LRBC apheresis: >=51g Hb, <5x10^6 Leuk

53
Q

Blood product QC
Plateletes

A

Plateletes: 5.5x10^10 plts, PH >=6.2
LR plateletes: 5.5x10^10, <8.2x10^5 leuks, PH >=6.2
pooled plts: <5.5x10^5 Leuks, >=6.2 PH
Apheresis plts: >=3x10^11

54
Q

Blood product QC
Apheresis gran

A

> = 1x10^10 granulocytes

55
Q

blood product QC
Cryo

A

> =150 mg Fibrinogen & min of 80 IU of FVIII

56
Q

Blood group systems
carbohydrate antigens

A

ABO, H, LE, I, P1PK, GLOB
Naturally occuring
usually IgM, can be IgG
React best at RT
Usually direct agglutination
Can react with complement

57
Q

Blood group systems
Protein antigens

A

FY, JK, KEL, Rh, MNS
immunogenic
usually IgG
react best at 37C
Attach to respective antigens, failing to demonstrate direct agglutination
AHG reagent used to demonstrate agg. (IAT)

58
Q

Hardy-Weinberg equation and work

A

p^2+2pq+q^2=1
p+q=1

if K+ is in 9% of the population, determine KK, Kk, kk
p^2+2pq+q^2 =1
q^2=1-(p^2+1pq)
(p^2=2pq) - K+ = .09
q^2=.91
q=sqrt .91=.95
p+q=1, p=1-.95
p=.05
Therefore
p^2 = .05^2 = .0025 is the frequency of KK
2pq = 2(.05x.95) = .0950 is the frequency of Kk
q^2 = .95^2 = .9025 is the frequency of kk

59
Q

ABO

A

Most important
non-immunogenic
Ch.9
A/B genes co-dominate, or dominant in the presence of O
A terminal sugar: N-acetylegalactoseamine
B terminalk sugar: Galactose

60
Q

H antigen

A

FUT1 Gene
Ch. 19
H terminal sugar: Fucose
H expression most to least : O>A2>b>A2B>A1>A1B

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