Block D Part 2: Post-Absorptive State Flashcards
What are the only 2 fats that are essential to our diet?
Linoleic acid [C18:2] and linolenic acid [C18:3]
(Lecture 2, Slide 4)
What 4 lipid-soluble vitamins are only present in dietary fat and require fat for absroption?
Vitamins A,D,E and K
(Lecture 2, Slide 4)
What does fat do to food in the mouth?
It lubricates it and contains most of the favour
(Lecture 2, Slide 4)
What are the 3 types of fat in our diet and body?
Triacylglycerols
Phospholipids
Cholesterol
(Lecture 2, Slide 5)
What are phospholipids made of?
2 fatty acids and a head group attached to glycerol
(Lecture 2, Slide 5)
What are 4 common head groups of phospholipids?
Phosphocholine
Phosphoethanolamine
Phosphoserine
Phosphoinositol
(Lecture 2, Slide 8)
What carries out digestion of fat?
Lipases
(Lecture 2, Slide 9)
What are 4 types of lipase?
Lingual lipase
Gastric lipase
Pancreatic lipase
Phospholipase
(Lecture 2, Slide 9)
Are lipids hydrophilic or hydrophobic?
Hydrophobic
(Lecture 2, Slide 10)
Are lipids insoluble or soluble in aqueous environments?
Insoluble
(Lecture 2, Slide 10)
What are needed to transport lipids around the body?
Lipoproteins
(Lecture 2, Slide 10)
What is the process of fatty acid oxidation called?
ß-oxidation
(Lecture 2, Slide 11)
What are the 5 major steps in the oxidation of fatty acids in the mitochondria?
Activation
Oxidation
Hydration
Oxidation
Thiolysis
(Lecture 2, Slide 11)
What occurs in the activation stage of oxidation of fatty acids?
The fatty acid forms a thioester bond with CoA and then gets transported into the mitochondria
(Lecture 2, Slide 11)
What occurs in the first oxidation stage of oxidation of fatty acids?
2H removed forming an additional C=C double bond
(Lecture 2, Slide 11)
What occurs in the hydration stage of oxidation of fatty acids?
H2O is added across the double bond
(Lecture 2, Slide 11)
What occurs in the 2nd oxidation stage of oxidation of fatty acids?
C-OH bond is converted into C=O
(Lecture 2, Slide 11)
What occurs in the thiolysis stage of oxidation of fatty acids?
Acetyl-CoA cleaved from activated end
(Lecture 2, Slide 11)
What carrier does the transport of fatty acids into the mitochondria use?
Carnitine
(Lecture 2, Slide 13)
What is carnitine?
An acyl-carrier that transports fatty acid chains
(Lecture 2, Slide 13)
How are fatty acyls (fatty acids) converted to acetyl-CoAs?
Steps 2-5 of the oxidation process is repeated until the entire chain is converted into acetyl-CoAs
(Lecture 2, Slide 15)
How much ATP does each ß-oxidation cycle produce?
14
(Lecture 2, Slide 15)
How does each ß-oxidation cycle produce 14 ATP?
NADH - 2.5ATP
FADH2 - 1.5ATP
Acetyl CoA - 10ATP (via TCA cycle generating NADH, FADH2, GTP)
(Lecture 2, Slide 15)
How many cycles of oxidation does palmitate (C16:0) need to undergo to be completely converted to Acetyl CoA?
7
(Lecture 2, Slide 15)
What is the total yield of ATP from palmitate (C16:0)?
106
(Lecture 2, Slide 15)
How is 106 ATP produced from palmitate (C16:0)?
14 ATP from each ß-oxidation cycle x 7 cycles needed to fully convert palmitate to acetyl CoAs = 108 ATP - 2 ATPs needed to activate the fatty acid = 106 ATP
(Lecture 2, Slide 15)
What 2 additional enzymes are required in the degradation of unsaturated fatty acids?
cis-D 3-Enoyl CoA isomerase and 2,4-Dienoyl CoA reductase
(Lecture 2, Slide 16)
Why are cis-D 3-Enoyl isomerase and 2,4-Dienyol CoA reductase needed to degrade unsaturated amino acids?
cis-D 3-Enoyl CoA isomerase is needed for one C=C double bond and both cis-D 3-Enoyl CoA and 2,4-Dienoyl CoA reductase are needed for 2 C=C double bonds
(Lecture 2, Slide 16)
What is an odd chain fatty acid?
A fatty acid containing an odd number of carbons in its hydrocarbon chain
(Lecture 2, Slide 17)
What do odd chain fatty acids yield at the end of degradation?
Propionyl-CoA (3C)
(Lecture 2, Slide 17)
How is propionyl-CoA generated at the end of odd chain fatty acid degradation converted to succinyl CoA?
By the addition of bicarbonate
(Lecture 2, Slide 17)
What happens to succinyl-CoA after it has been converted from propionyl-CoA?
It enters the TCA cycle
(Lecture 2, Slide 17)
What is gluconeogenesis?
The conversion of pyruvate into glucose
(Lecture 2, Slide 20)
Where does pyruvate mainly occur?
In the liver
(Lecture 2, Slide 20)
What precursors can enter the gluconeogenesis pathway at later intermediates?
Non-carbohydrate precursors
(Lecture 2, Slide 20)
What are 4 major non-carbohydrate precursors?
Lactate
Propionate
Amino acids
Glycerol
(Lecture 2, Slide 20)
How are propionate and glycerol generated?
From hydrolysis of triacylglycerols
(Lecture 2, Slide 20)