Block 1 RUSVM Flashcards
Carbohydrates consist of what molecules?
Carbon, hydrogen and oxygen
3 examples of monosaccharides
Glucose, fructose, galactose
What % of dry matter are carbohydrates in plant based foods
75%
Carbohydrates are the 2nd most available energy source. T or F
F. They are the most available
Proteins are bio/macromolecules consisting of what kind of AA
1 or more long chain AA
AA are needed in the diet to produce what?
Proteins
Aa to glucose is referred to as what?
Gluconeogenesis
What are some (6) functional diversities of protein?
A. Cell membrane structure and transport
B. Enzymes
C. Hormones
D. Catalyzing metabolic reactions
E. DNA replication
F. Structure to cells, organs
Tissue proteins need continuous replacement due to what?
Metabolic turnover
What is the biological value of dietary protein? How is it measured?
The ability of a specific dietary protein to supply AA in the relative amount required for protein synthesis.
Measured by Nitrogen balance
Influenced by essential AA content of the protein in question
Crude protein is an estimate of ____ that is based on an average _____ content of protein.
Protein, nitrogen 16%
Crude protein is not accurate because it can have non-protein nitrogen feedstuff like:
Ammonia, urea, nitrate
Melamine is a molecule with 66% nitrogen. Melanin can be added to feedstuff to increase crude protein analysis making food more expensive but it is what to animals?
Toxic!
There are 20 protgenic Amino Acids and 10 essential AA. What are the 10 essential AA?
PVT TIM HALL
phenylalanine
Valine
Threonine
Tryptophan
Isoleucine
Methionine
Histidine
Arginine
Leucine
Lysine
Taurine is a non-proteinogenic AA that is only present in animals as a free AA. What organs is it important for?
Brain, heart, GI
Organic vitamin compounds are required in small amounts for normal metabolism. Many are destroyed by what? (4)
Oxidation, heat, light, or certain meds
Why is it essential to obtain vitamins from the diet?
Most vitamins cannot be synthesized by animal cells.
Exceptions are niacin and vit D
Vitamin A comes from what?
Food from Animals. Plants do not have vit A
Vitamin A
Function:
Deficiency:
Excess:
Function: vision, maintenance of mucosal surfaces
Deficiency: poor growth, inc risk of disease, infertility, right blindness
Excess: liver fibrosis, bone and eye abnormalities, hair loss, neurological signs
Vitamin D
Is limited in feedstuff, but abundant in fish. Most animals can synthesize vit D3 with exposure to what?
Sunlight!
Vitamin D
Function:
Deficiency:
Excess:
Function: absorption and metabolism of Ca and K
Deficiency: rickets in young or osteomalacia in adults
Excess: kidney damage, hypercalcemia, hypermineralization, diarrhea, polyuria, hypertension, vomiting
Vitamin E (tocopherol)
Function
Deficiency
Excess
- Widely distributed in feedstuffs (especially vegetable oils)
- Functions:
a. Antioxidant (against oxidation or polyunsaturated fatty acids in cell membranes)
i. Oxidation → wear and tear on body (aging) - Deficiency: Anemia, impaired reproduction, hepatic damage,
muscular degeneration (white muscle disease) - Excess: Vit E becomes a free radical species and is toxic
a. Excess oxidation → hepatic damage
Vitamin K
Function
Deficiency
Excess
- Mammals can’t synthesize (plants, algae, bacteria can) a. Alfalfa is a good source
- Function: Coenzyme of procoagulation factors (II, VII, IX, X)
- Deficiency: hemorrhagic disease
a. Impaired fat absorption
b. Low reserves in neonates
c. Spoiled clover→ coumarin→ converted to dicumerol→
inhibits recycling of vit K→ hemorrhage - Excess: erythrocyte instability, hemolysis, potentially fatal anemia
(Vit K is an oxidant)
Vitamin B1 (thiamine)
Function
Deficiency
- Abundant in legumes, nuts whole grains, yeast and animal products
- Functions: coenzyme for reactions (transfer of aldehyde unit)
- Deficiency: Neurologic problems, beriberi
a. Cats: raw seafood contains thiaminase
b. Young ruminants: cerebrocortical necrosis (star-gazing)
i. Change in pH causes rumen production insufficiency (tx w/ thiamine inj)
c. Horses: Bracken fern contains thiaminase
Vitamin B12 (cobalamin)
Is found where?
Function
Deficiency
- Synthesized excessively by microorganisms
a. Vitamin not required by ruminants if Cobalt is sufficient
b. Absent in plants (vegetarians require supplements)
c. Present in animal tissues
2. Absorption requires intrinsic factors
3. Functions:
a. Detoxify homocysteine
b. Maintain intracellular stores of methionine
c. Regenerate folate
d. Synthesis of purine, pyrimidine and nucleic acid
e. Synthesis of succinyl-CoA (important for inclusion into
krebs cycle and glucose production in ruminants)
4. Deficiency:
a. Non-ruminants: fed plant source diet with inadequate cobalt intake → impairs methionine synthesis → impaired DNA synthesis → impaires formation of erythrocytes
i. May see neurologic signs
Vitamin B9 (folate)
Found
Function
Deficiency
- Synthesized by plants and microbes
- Distributed in plants and animal products
- Function: contains one-carbon units → contributes to purine rings
→ essential for DNA synthesis - Deficiency:
a. Seen in pregnant and lactating animals
b. Seen in B12 deficient animals
i. B12 converts B9 to its active form
c. Megaloblastic anemia, neural tube defects, poor growth,
reduced egg hatchability
What is a lipid?
i. Hydrocarbon compounds soluble in organic solvents (generally not water soluble)
ii. Highly reduced (high proportion of hydrogen atoms)
iii. Highly palatable
What are some functions of lipids? (5)
- Energy storage
a. Insulation and thermoregulation - Structural components of cell membranes
- Signaling molecules
a. Inflammatory cascade
b. Insulin resistance - Act as carrier of nutrients
- A source of essential fatty acids
Fats contain how much more energy of carbohydrates per gram?
Contain 2.25x the energy of carbohydrates per gram
1. 1gcarbs=4kcal
2. 1gfat=9kcal
Fats are what?
esters of fatty acids and glycerol
What is a fatty acid?
carboxylic acids with long, unbranched aliphatic tails
What is a saturated FA?
Saturated (single bonds with H+)
i. Short-chain fatty acids
ii. Medium-chain fatty acids
iii. Long-chain fatty acids
What is an unsaturated FA?
ans?
Cis?
Conjugated?
Monounsaturated?
Polyunsaturated?
Unsaturated (double bods)
Trans: sequential H are facing opposite sides of the
bond
Molecule is a straight line → compact fats
(firmer)
Cis: adjacent H to the bond face the same way
Causes bending of the molecule → occupy more space → less dense (oily/soft)
Conjugated: 2 double bonds with one Carbon between them
Monounsaturated: single double bond
Polyunsaturated: multiple double bonds
Structure of FA is?
a. Carbons are counted from the carboxylic acid on the end
(delta)
i. Side that’s attached to glycerol backbone (where
biochemical reactions happen)
ii. Omega carbon is opposite of the carboxylic acid
How are triglycerides formed?
- Formed by combining glycerol with 3 fatty acid molecules
- Mostly 16-20 carbon atoms
Short chain fatty acids are made up on how many carbons? Are they water soluble? Where are they found?
- 2-5 carbon atoms
- water soluble
- Found in rumen & blood of ruminants
- Crucial for ruminant nutrition
- Butyrate = important for colonic health
Medium chain fatty acids are made up on how many carbons? Are they water soluble? Where are they found?
- 6-12 carbon atoms
- Water solubility varies
- Solubility → absorbed from SI into portal vein
- Common in plants
- Make up about 10-20% of fatty acid in milk of domestic animals
Long chain fatty acids are made up on how many carbons? Are they water soluble? Where are they found?
- > 12 carbon atoms
- Insoluble in water
- Palmitic acid is the most common LCFA in plants and animals
a. Most common monounsaturated is oleic
b. Most common polyunsaturated
c. Activates JNK
d. Insulin resistance (inhibits insulin gene transcription)
e. Mediates obesity-induced inflammation - PUFAs prone to oxidation during storage and processing
What are essential fatty acids?
Not synthesized by mammals, but required for metabolism (mammals lack
desaturase enzyme effect beyond C9)
What are important % of water loss? (3).
Loss of water:
i. 5% → loss of appetite, restlessness
ii. 7% → metabolic disorders
iii. 15-20% → death
What are functions of water loss?
Functions:
i. Body temperature regulation
ii. Solvent (for nutrients and metabolites)
iii. Ionizing power (biochemical reactions)
iv. Transport of nutrients and waste products
v. Lubricant
vi. Cushioning of organs, fetuses, etc
What is gross energy? (GE)
Total energy of given mass of feed determined as its heat of combustion
(bomb calorimetry)
What is Digestible Energy (DE)?
Digestible Energy (DE) → for digestion
i. GE - fecal energy
What is Metabolizable Energy (ME)?
Metabolizable Energy (ME) → for metabolic processes
i. DE - urinary energy - methane energy
1. Roughly— ruminants ME=0.82DE, nonruminants ME=0.95DE
What is Net Energy (NE)?
Net Energy (NE) → available for maintenance in the body i. ME - heat increment
ii. Way less than the gross energy
iii. Net energy is used first to meet the animal’s maintenance energy
requirement
What is Retained Energy (RE)?
Retained Energy (RE)
i. Any surplus deposited in body tissues (growth) or products (milk, eggs, etc.)
What are the 4 types of Dry matter and that do they consist of?
Dry matter i. Ash
1. Minerals ii. Ether extract
1. Dietary Fats
2. Fat soluble vitamins
iii. Crude Protein
1. Analysis of Nitrogen by Kjeldahl N analysis
2. 16% Nitrogen measured allows for educated guess of protein
amount (crude)
3. Proteins and AA
iv. Nitrogen Free Extract (NFE)
1. Difference that is left that isn’t Nitrogen
2. Sugars, starch, glycogens
3. Determines crude fibers
What are 3 neutral detergent factors and how are they extracted?
Extraction with boiling neutral detergent
1. Cell content
2. Cell wall
a. Neutral detergent factor (NDF)
i. Hemicellulose (most digestible)
ii. Cellulose (intermediate digestibility)
iii. Lignin (least digestible)
What does it mean to extract with acid detergent and what are 2 factors?
Extraction with acid detergent (separates out the hemicellulose) 1. Hemicellulose
2. Acid detergent Factor (ADF) a. Cellulose
b. Lignin
In proximate (Weende) analysis, dietary fats will be found in which component?
Ether extract
What component of food is in the ash?
Minerals
What component of food is in the ether extract?
Dietary fats
Why do we call the Proximate Analysis of protein “crude protein”?
It is an estimate
Differentiate the different types of Nutrients:
Vitamins C which is also called ?
Vitamin C (Ascorbic acid)
Can all animals synthesize Ascorbic Acid? who cant? what result?
Guinea pigs cannot synthesize ascorbic acid (dependent on Vit C
in diet)
what does a vitamin c deficiency lead to?
Vit C deficiency → scurvy (SQ hemorrhage)
what process is vitamin c important to?
Important in Collagen synthesis
Differentiate the different types of Nutrients: What is vitamin B2 also called?
Vitamin B2 (Riboflavin)
Vitamin B2 (Riboflavin)
Cofactors in….
Cofactors in oxidoreductase enzymes
Vitamin B2 (Riboflavin) Important in synthesis of….
Important in synthesis of cholesterol, steroids, Vit D
Vitamin B2 (Riboflavin)
Deficiency →
Deficiency → inappetance, lesions of mouth, dermatitis,
photophobia, curled toe paralysis in chicks
Vitamin B2 (Riboflavin)
can this be quickly depleted? why?
Takes a long time to deplete this vitamin ( tightly bound to
proteins, turnover rate is low)
Differentiate the different types of Nutrients:Vitamin B3 also called…
Vitamin B3 (Niacin)
Vitamin B3 (Niacin)
Tryptophan can be converted to…
what result?
Tryptophan can be converted to nicotinate but efficiency is low
Vitamin B3 (Niacin)
component of what and what?
Component of NAD and NADP
Vitamin B3 (Niacin)
Deficiency →
what result in pigs?
Deficiency → “pellagra” : dermatitis, diarrhea, dementia, death (4
D’s)
Pigs: dermatitis, enteritis (vomiting and diarrhea), anorexia
Differentiate the different types of Nutrients:Vitamin B5 also called?
Vitamin B5 (Pantothenic acid)
Vitamin B5 (Pantothenic acid)
Component of ?
Component of Coenzyme A
Differentiate the different types of Nutrients: Vitamin B5 (Pantothenic acid) Acylcarrier protein in….
Acylcarrier protein in fatty acid synthesis
Vitamin B5 (Pantothenic acid)
Metabolism of…
Metabolism of nucleic acids and amino acids
Vitamin B5 (Pantothenic acid)
Deficiency in pigs and poultry →
Deficiency in pigs and poultry → poor growth, dermatitis and
altered gait (goose stepping incoordination)
Differentiate the different types of Nutrients:Vitamin B6 also called…
Vitamin B6 (Pyridoxine)
Vitamin B6 (Pyridoxine)
Coenzyme in…
Coenzyme in amino acid metabolism
Vitamin B6 (Pyridoxine)
Deficiency →
Deficiency → hyperammonemia → neurologic signs
Differentiate the different types of Nutrients: vitamin Biotin
what body part is it found?
Feet, claws, hooves
Biotin
Coenzyme of
Coenzyme of 4 ATP-dependent carboxylases
Biotin
is deficientcy common? what result?
Deficiency is rare but → hoof lesions, dry scaly skin, imparied
growth
Differentiate the different types of Nutrients:
Vitamins
Quasi-vitamins
what are hey required for?
are they considered vitamins?
Required for nutrition but not regarded as vitamins
Quasi-vitamins
what are some examples?
who are they supplimented for?
Ex) choline, carnitine, myo-inositol
a. Supplement in dairy animals
Differentiate the different types of Nutrients:
a. Vitamins
Name 7
i. Vitamin C (Ascorbic acid)
ii. Vitamin B2 (Riboflavin)
iii. Vitamin B3 (Niacin)
iv. Vitamin B5 (Pantothenic acid)
v. Vitamin B6 (Pyridoxine)
vi. Biotin
vii. Quasi-vitamins
Differentiate the different types of Nutrients:
Minerals (Ash)
define:
Minerals: inorganic elements present in food
Differentiate the different types of Nutrients:
Minerals (Ash)
do they synthesize or degrade?
Neither synthesized or degrades by animals or microbes
Differentiate the different types of Nutrients:
Minerals (Ash)
what do most minerals form?
Most minerals form complexes with proteins to exert their effects
Differentiate the different types of Nutrients:
Minerals (Ash)
can Minerals ever affect anything? if so what? what result?
Some minerals affect absorption and function of other minerals
a. Some can regulate gene expression
Differentiate the different types of Nutrients:
Minerals (Ash)
what are the 2 general functions, examples?
General functions of minerals
a. Major structural components: teeth, bones
b. Electrolytes essential for electric charges (muscle function)
Differentiate the different types of Nutrients:
Minerals (Ash)
Major minerals are also called ?
why?
examples?
Major minerals (macrominerals): required in large amounts
a. Calcium, phosphorus, sodium, chlorine, potassium, sulfur,
magnesium
Differentiate the different types of Nutrients:
Minerals (Ash)
Minor/trace minerals are called what?
why?
examples?
Minor/trace minerals (microminerals): required in trace amounts
a. Iron, copper, cobalt, manganese, zinc, iodine, selenium,
molybdenum, chromium, fluorine, others
Differentiate the different types of Nutrients:
Minerals (Ash)
what are some essential but potentially toxic minerals? (4)
Essential but potentially toxic minerals: Fluorine, selenium
molybdenum, copper
Differentiate the different types of Nutrients:
Minerals (Ash)
what are some always toxic minerals? (3)
Always toxic minerals: lead, mercury, cadmium
Differentiate the different types of Nutrients:
ii. Macrominerals
name one:
Macrominerals
1. Calcium (Ca)
Differentiate the different types of Nutrients:
ii. Macrominerals
1. Calcium (Ca)
a. Functions: Name 6
i. Skeletal tissues
ii. Conductive of nerve impulses
iii. Skeletal and cardiac muscle
iv. Immune cells (WBC) require Ca+
v. Blood clotting
vi. Component of milk:
Differentiate the different types of Nutrients:
ii. Macrominerals
1. Calcium (Ca)
a. Functions:
i. Skeletal tissues
how much? what percent?
tissues (99% of Ca+ in bones)